Gene description for CTPS2
Gene name CTP synthase 2
Gene symbol CTPS2
Other names/aliases -
Species Homo sapiens
 Database cross references - CTPS2
ExoCarta ExoCarta_56474
Vesiclepedia VP_56474
Entrez Gene 56474
HGNC 2520
MIM 300380
UniProt Q9NRF8  
 CTPS2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for CTPS2
Molecular Function
    CTP synthase activity GO:0003883 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    pyrimidine nucleotide metabolic process GO:0006220 TAS
    CTP biosynthetic process GO:0006241 IBA
    pyrimidine nucleobase biosynthetic process GO:0019856 IBA
    'de novo' CTP biosynthetic process GO:0044210 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HTP
    cytosol GO:0005829 TAS
    cytoophidium GO:0097268 IBA
 Experiment description of studies that identified CTPS2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CTPS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SGCA  
Affinity Capture-MS Homo sapiens
3 MCM3 4172
Co-fractionation Homo sapiens
4 OTUD5 55593
Affinity Capture-MS Homo sapiens
5 SNX3 8724
Affinity Capture-MS Homo sapiens
6 SPRTN  
Affinity Capture-MS Homo sapiens
7 CTPS2 56474
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 CTBP2 1488
Two-hybrid Homo sapiens
9 RPA2 6118
Proximity Label-MS Homo sapiens
10 RPL37A 6168
Two-hybrid Homo sapiens
11 SLC2A1 6513
Affinity Capture-MS Homo sapiens
12 TYROBP  
Affinity Capture-MS Homo sapiens
13 TMEM9B 56674
Affinity Capture-MS Homo sapiens
14 IL22RA2  
Affinity Capture-MS Homo sapiens
15 TNFAIP6 7130
Affinity Capture-MS Homo sapiens
16 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
17 PRKDC 5591
Co-fractionation Homo sapiens
18 CHST10  
Affinity Capture-MS Homo sapiens
19 KIF23 9493
Affinity Capture-MS Homo sapiens
20 FAM174A 345757
Affinity Capture-MS Homo sapiens
21 MRPS23 51649
Affinity Capture-MS Homo sapiens
22 MRPS24 64951
Affinity Capture-MS Homo sapiens
23 SUV39H1  
Affinity Capture-MS Homo sapiens
24 MTMR2 8898
Co-fractionation Homo sapiens
25 C1orf111  
Affinity Capture-MS Homo sapiens
26 CRCP  
Affinity Capture-MS Homo sapiens
27 LRSAM1 90678
Co-fractionation Homo sapiens
28 SFN 2810
Co-fractionation Homo sapiens
29 COL9A1  
Affinity Capture-MS Homo sapiens
30 FGFR1 2260
Two-hybrid Homo sapiens
31 EHD1 10938
Co-fractionation Homo sapiens
32 METTL10  
Affinity Capture-MS Homo sapiens
33 RHOH  
Affinity Capture-MS Homo sapiens
34 YWHAB 7529
Co-fractionation Homo sapiens
35 GTF2I 2969
Co-fractionation Homo sapiens
36 ENPP6 133121
Affinity Capture-MS Homo sapiens
37 SPG21 51324
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
38 TSGA10IP  
Affinity Capture-MS Homo sapiens
39 SHISA3  
Affinity Capture-MS Homo sapiens
40 ROBO3  
Affinity Capture-MS Homo sapiens
41 PSMD14 10213
Co-fractionation Homo sapiens
42 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
43 VSIG4  
Affinity Capture-MS Homo sapiens
44 RAMP3  
Affinity Capture-MS Homo sapiens
45 DDX5 1655
Co-fractionation Homo sapiens
46 NTRK1 4914
Affinity Capture-MS Homo sapiens
47 GPR182  
Affinity Capture-MS Homo sapiens
48 AQP12B  
Affinity Capture-MS Homo sapiens
49 CACNG4  
Affinity Capture-MS Homo sapiens
50 UPP1 7378
Affinity Capture-MS Homo sapiens
51 ACTN1 87
Co-fractionation Homo sapiens
52 BACE2 25825
Affinity Capture-MS Homo sapiens
53 APLNR  
Affinity Capture-MS Homo sapiens
54 SUGP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 NASP 4678
Affinity Capture-MS Homo sapiens
56 ICAM2 3384
Affinity Capture-MS Homo sapiens
57 XPO1 7514
Affinity Capture-MS Homo sapiens
58 NPRL3  
Affinity Capture-MS Homo sapiens
59 ASAH1 427
Affinity Capture-MS Homo sapiens
60 TBX20 57057
Affinity Capture-MS Homo sapiens
61 CUL3 8452
Affinity Capture-MS Homo sapiens
62 NPPA  
Affinity Capture-MS Homo sapiens
63 MTNR1A  
Two-hybrid Homo sapiens
64 CTPS1 1503
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 NUCB1 4924
Co-fractionation Homo sapiens
67 GPR45  
Affinity Capture-MS Homo sapiens
68 DLST 1743
Co-fractionation Homo sapiens
69 DHPS 1725
Co-fractionation Homo sapiens
70 PLIN3 10226
Co-fractionation Homo sapiens
71 CD244 51744
Affinity Capture-MS Homo sapiens
72 CDK20  
Affinity Capture-MS Homo sapiens
73 RNF2  
Affinity Capture-MS Homo sapiens
74 KTN1 3895
Co-fractionation Homo sapiens
75 RAB28 9364
Affinity Capture-MS Homo sapiens
76 PRSS22 64063
Affinity Capture-MS Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 NAAA  
Affinity Capture-MS Homo sapiens
79 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
80 EHD4 30844
Co-fractionation Homo sapiens
81 MCM5 4174
Co-fractionation Homo sapiens
82 RNASEH2C 84153
Affinity Capture-MS Homo sapiens
83 SLAMF1 6504
Affinity Capture-MS Homo sapiens
84 TIGD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 HSF1  
Co-fractionation Homo sapiens
86 METTL1 4234
Co-fractionation Homo sapiens
87 EPHA2 1969
Proximity Label-MS Homo sapiens
88 SERBP1 26135
Affinity Capture-MS Homo sapiens
89 RPL23A 6147
Two-hybrid Homo sapiens
90 VIPR1  
Affinity Capture-MS Homo sapiens
91 C9orf72  
Affinity Capture-MS Homo sapiens
92 ATOH1  
Affinity Capture-MS Homo sapiens
93 WARS 7453
Co-fractionation Homo sapiens
94 CLEC4E  
Affinity Capture-MS Homo sapiens
95 DKKL1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CTPS2 is involved
PathwayEvidenceSource
Interconversion of nucleotide di- and triphosphates TAS Reactome
Metabolism TAS Reactome
Metabolism of nucleotides TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here