Gene description for DHPS
Gene name deoxyhypusine synthase
Gene symbol DHPS
Other names/aliases DHS
DS
MIG13
Species Homo sapiens
 Database cross references - DHPS
ExoCarta ExoCarta_1725
Vesiclepedia VP_1725
Entrez Gene 1725
HGNC 2869
MIM 600944
UniProt P49366  
 DHPS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DHPS
Molecular Function
    protein binding GO:0005515 IPI
    deoxyhypusine synthase activity GO:0034038 IBA
    deoxyhypusine synthase activity GO:0034038 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    translation GO:0006412 TAS
    spermidine metabolic process GO:0008216 IBA
    spermidine metabolic process GO:0008216 IDA
    positive regulation of cell population proliferation GO:0008284 TAS
    peptidyl-lysine modification to peptidyl-hypusine GO:0008612 IDA
    positive regulation of T cell proliferation GO:0042102 IEA
    glucose homeostasis GO:0042593 IEA
    spermidine catabolic process GO:0046203 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DHPS in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DHPS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 EXOSC7 23016
Affinity Capture-MS Homo sapiens
3 CTPS2 56474
Co-fractionation Homo sapiens
4 ACTR2 10097
Co-fractionation Homo sapiens
5 REL 5966
Two-hybrid Homo sapiens
6 BRCA1 672
Two-hybrid Homo sapiens
7 BYSL 705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SRSF2 6427
Affinity Capture-MS Homo sapiens
9 ZNF414  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 MYEOV2 150678
Affinity Capture-MS Homo sapiens
11 SURF2  
Co-fractionation Homo sapiens
12 ANKMY2 57037
Co-fractionation Homo sapiens
13 EXOSC2 23404
Affinity Capture-MS Homo sapiens
14 TGFB1 7040
Affinity Capture-MS Homo sapiens
15 PPP2R5D 5528
Co-fractionation Homo sapiens
16 ACTR3 10096
Proximity Label-MS Homo sapiens
17 ANP32B 10541
Co-fractionation Homo sapiens
18 SPANXN4  
Affinity Capture-MS Homo sapiens
19 SRRM2 23524
Affinity Capture-MS Homo sapiens
20 NUDT18  
Two-hybrid Homo sapiens
21 PTPRR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 EXOSC4 54512
Affinity Capture-MS Homo sapiens
23 TULP3 7289
Affinity Capture-MS Homo sapiens
24 VASH1  
Two-hybrid Homo sapiens
25 PRTN3 5657
Affinity Capture-MS Homo sapiens
26 NIF3L1 60491
Two-hybrid Homo sapiens
27 MAPK1 5594
Affinity Capture-MS Homo sapiens
28 PLA2G4A 5321
Co-fractionation Homo sapiens
29 SRP9 6726
Co-fractionation Homo sapiens
30 DPP8 54878
Co-fractionation Homo sapiens
31 MAPK3 5595
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SPATA24  
Affinity Capture-MS Homo sapiens
33 RPL9 6133
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 SAE1 10055
Co-fractionation Homo sapiens
35 LGR5 8549
Affinity Capture-MS Homo sapiens
36 FAM19A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 SP110  
Affinity Capture-MS Homo sapiens
38 SAFB2 9667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 WDYHV1  
Two-hybrid Homo sapiens
40 CFAP46  
Affinity Capture-MS Homo sapiens
41 C9orf170  
Affinity Capture-MS Homo sapiens
42 FAM133A 286499
Affinity Capture-MS Homo sapiens
43 ZNF138  
Two-hybrid Homo sapiens
44 TIRAP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 CDK17 5128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SFPQ 6421
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SPHK1 8877
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ARPC1B 10095
Co-fractionation Homo sapiens
49 PTPRH 5794
Two-hybrid Homo sapiens
50 CUL3 8452
Affinity Capture-MS Homo sapiens
51 ARF6 382
Affinity Capture-MS Homo sapiens
52 PTPN7 5778
Affinity Capture-MS Homo sapiens
53 ARHGAP15 55843
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 EPS8L1 54869
Affinity Capture-MS Homo sapiens
55 RPA3 6119
Co-fractionation Homo sapiens
56 DHPS 1725
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
57 PTPDC1  
Affinity Capture-MS Homo sapiens
58 PLIN3 10226
Co-fractionation Homo sapiens
59 KCNN4 3783
Affinity Capture-MS Homo sapiens
60 PTPN23 25930
Proximity Label-MS Homo sapiens
61 KIAA1522 57648
Affinity Capture-MS Homo sapiens
62 DRG2 1819
Proximity Label-MS Homo sapiens
63 HUWE1 10075
Affinity Capture-MS Homo sapiens
64 ZRANB1 54764
Affinity Capture-MS Homo sapiens
65 DUSP19  
Affinity Capture-MS Homo sapiens
66 PADI6  
Affinity Capture-MS Homo sapiens
67 DUSP28  
Affinity Capture-MS Homo sapiens
68 LRRC6  
Affinity Capture-MS Homo sapiens
69 RAB27B 5874
Two-hybrid Homo sapiens
70 DLX1  
Affinity Capture-MS Homo sapiens
71 CCHCR1  
Affinity Capture-MS Homo sapiens
72 PLCD3 113026
Affinity Capture-MS Homo sapiens
73 ZNF276 92822
Affinity Capture-MS Homo sapiens
74 EIF5A2 56648
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
75 ANAPC2 29882
Proximity Label-MS Homo sapiens
76 SUSD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 C16orf72 29035
Affinity Capture-MS Homo sapiens
78 HSF1  
Co-fractionation Homo sapiens
79 HNRNPF 3185
Co-fractionation Homo sapiens
80 EIF5A 1984
Two-hybrid Homo sapiens
81 KRAS 3845
Negative Genetic Homo sapiens
82 AAMP 14
Co-fractionation Homo sapiens
83 TRAV20  
Affinity Capture-MS Homo sapiens
84 KCNAB3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 ARIH1 25820
Co-fractionation Homo sapiens
86 PROSER2 254427
Affinity Capture-MS Homo sapiens
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