Gene description for SPHK1
Gene name sphingosine kinase 1
Gene symbol SPHK1
Other names/aliases SPHK
Species Homo sapiens
 Database cross references - SPHK1
ExoCarta ExoCarta_8877
Vesiclepedia VP_8877
Entrez Gene 8877
HGNC 11240
MIM 603730
UniProt Q9NYA1  
 SPHK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SPHK1
Molecular Function
    magnesium ion binding GO:0000287 IDA
    lipid kinase activity GO:0001727 TAS
    DNA binding GO:0003677 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IDA
    ATP binding GO:0005524 IDA
    lipid binding GO:0008289 IDA
    sphingosine kinase activity GO:0008481 IDA
    acetyltransferase activity GO:0016407 ISS
    D-erythro-sphingosine kinase activity GO:0017050 IBA
    D-erythro-sphingosine kinase activity GO:0017050 IDA
    D-erythro-sphingosine kinase activity GO:0017050 TAS
    sphingosine-1-phosphate receptor activity GO:0038036 IMP
    protein phosphatase 2A binding GO:0051721 IPI
Biological Process
    blood vessel development GO:0001568 IEA
    sphingosine-1-phosphate receptor signaling pathway GO:0003376 IEA
    protein acetylation GO:0006473 ISS
    sphingosine metabolic process GO:0006670 IDA
    inflammatory response GO:0006954 IEA
    brain development GO:0007420 IEA
    cell population proliferation GO:0008283 IEA
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 IMP
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 IDA
    calcium-mediated signaling GO:0019722 IDA
    regulation of endocytosis GO:0030100 IDA
    sphingolipid biosynthetic process GO:0030148 TAS
    positive regulation of cell growth GO:0030307 IDA
    positive regulation of cell migration GO:0030335 IDA
    positive regulation of protein ubiquitination GO:0031398 IDA
    regulation of interleukin-1 beta production GO:0032651 IEA
    positive regulation of interleukin-17 production GO:0032740 ISS
    response to tumor necrosis factor GO:0034612 IDA
    intracellular signal transduction GO:0035556 TAS
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IDA
    negative regulation of apoptotic process GO:0043066 IBA
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of apoptotic process GO:0043066 TAS
    positive regulation of angiogenesis GO:0045766 IDA
    positive regulation of mitotic nuclear division GO:0045840 IEA
    positive regulation of mitotic cell cycle GO:0045931 IDA
    positive regulation of smooth muscle contraction GO:0045987 IDA
    sphingosine biosynthetic process GO:0046512 IBA
    sphingosine biosynthetic process GO:0046512 IGI
    sphingosine biosynthetic process GO:0046512 IMP
    sphingoid catabolic process GO:0046521 NAS
    positive regulation of fibroblast proliferation GO:0048146 IDA
    regulation of phagocytosis GO:0050764 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISS
    cellular response to hydrogen peroxide GO:0070301 IEA
    cellular response to growth factor stimulus GO:0071363 IBA
    DNA biosynthetic process GO:0071897 IEA
    regulation of neuroinflammatory response GO:0150077 ISS
    negative regulation of ceramide biosynthetic process GO:1900060 IDA
    positive regulation of p38MAPK cascade GO:1900745 IDA
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IMP
    regulation of microglial cell activation GO:1903978 ISS
    regulation of endosomal vesicle fusion GO:1905364 ISS
Subcellular Localization
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    clathrin-coated pit GO:0005905 IDA
    membrane GO:0016020 IBA
    endocytic vesicle GO:0030139 ISS
    early endosome membrane GO:0031901 IDA
    intracellular membrane-bounded organelle GO:0043231 IBA
    presynapse GO:0098793 IDA
    presynapse GO:0098793 IMP
    presynapse GO:0098793 ISS
 Experiment description of studies that identified SPHK1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SPHK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MBTPS1 8720
Biochemical Activity Homo sapiens
2 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
3 DCXR 51181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 WRAP53  
Affinity Capture-MS Homo sapiens
5 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 CTU1 90353
Affinity Capture-MS Homo sapiens
7 PPIP5K1 9677
Affinity Capture-MS Homo sapiens
8 MAP1S 55201
Affinity Capture-MS Homo sapiens
9 SMARCD1 6602
Affinity Capture-MS Homo sapiens
10 KLHL8  
Affinity Capture-MS Homo sapiens
11 BAG5 9529
Affinity Capture-MS Homo sapiens
12 TUBB8 347688
Affinity Capture-MS Homo sapiens
13 TRAF6 7189
Affinity Capture-Western Homo sapiens
14 ANKRD40 91369
Affinity Capture-MS Homo sapiens
15 SMG8 55181
Affinity Capture-MS Homo sapiens
16 CTU2 348180
Affinity Capture-MS Homo sapiens
17 DDX58 23586
Affinity Capture-RNA Homo sapiens
18 ALDH3B1 221
Affinity Capture-MS Homo sapiens
19 TOMM22 56993
Affinity Capture-MS Homo sapiens
20 IL12RB1  
Positive Genetic Homo sapiens
21 IL10 3586
Positive Genetic Homo sapiens
22 DHPS 1725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 RDH11 51109
Affinity Capture-MS Homo sapiens
24 TTC1 7265
Affinity Capture-MS Homo sapiens
25 RPS6KC1  
Affinity Capture-Western Homo sapiens
26 CCZ1 51622
Affinity Capture-MS Homo sapiens
27 LEMD3  
Affinity Capture-MS Homo sapiens
28 PPM1G 5496
Affinity Capture-MS Homo sapiens
29 CYLD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 RARG  
Two-hybrid Homo sapiens
31 EP300 2033
Biochemical Activity Homo sapiens
32 QTRTD1  
Affinity Capture-MS Homo sapiens
33 BIRC3 330
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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