Gene description for SPHK1
Gene name sphingosine kinase 1
Gene symbol SPHK1
Other names/aliases SPHK
Species Homo sapiens
 Database cross references - SPHK1
ExoCarta ExoCarta_8877
Vesiclepedia VP_8877
Entrez Gene 8877
HGNC 11240
MIM 603730
UniProt Q9NYA1  
 SPHK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SPHK1
Molecular Function
    DNA binding GO:0003677 IDA
    D-erythro-sphingosine kinase activity GO:0017050 IDA
    sphingosine-1-phosphate receptor activity GO:0038036 IMP
    protein phosphatase 2A binding GO:0051721 IPI
    protein binding GO:0005515 IPI
    magnesium ion binding GO:0000287 ISS
    ATP binding GO:0005524 IDA
    calmodulin binding GO:0005516 IDA
    sphinganine kinase activity GO:0008481 IDA
Biological Process
    sphingosine metabolic process GO:0006670 IDA
    cellular response to hydrogen peroxide GO:0070301 IEA
    positive regulation of neurotransmitter secretion GO:0001956 IEA
    positive regulation of protein ubiquitination GO:0031398 IDA
    sphingolipid metabolic process GO:0006665 TAS
    small molecule metabolic process GO:0044281 TAS
    negative regulation of apoptotic process GO:0043066 TAS
    brain development GO:0007420 IEA
    signal transduction GO:0007165 TAS
    response to amine GO:0014075 IEA
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    protein folding GO:0006457 TAS
    response to interleukin-1 GO:0070555 IEA
    calcium-mediated signaling GO:0019722 IDA
    positive regulation of neuron projection development GO:0010976 IEA
    positive regulation of mitotic cell cycle GO:0045931 IDA
    response to magnesium ion GO:0032026 IEA
    inflammatory response GO:0006954 IEA
    positive regulation of angiogenesis GO:0045766 IDA
    sphingosine biosynthetic process GO:0046512 IMP
    positive regulation of smooth muscle contraction GO:0045987 IDA
    cellular protein metabolic process GO:0044267 TAS
    blood vessel development GO:0001568 IEA
    positive regulation of cell migration GO:0030335 IDA
    cyclooxygenase pathway GO:0019371 IEA
    response to progesterone GO:0032570 IEA
    intracellular signal transduction GO:0035556 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    regulation of interleukin-1 beta production GO:0032651 IEA
    'de novo' posttranslational protein folding GO:0051084 TAS
    lipid phosphorylation GO:0046834 IEA
    female pregnancy GO:0007565 IEA
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 IDA
    cellular response to starvation GO:0009267 IEA
    positive regulation of cell growth GO:0030307 IDA
    sphingolipid biosynthetic process GO:0030148 TAS
    positive regulation of fibroblast proliferation GO:0048146 IDA
    sphingoid catabolic process GO:0046521 NAS
    sphingosine-1-phosphate signaling pathway GO:0003376 IMP
    positive regulation of NF-kappaB import into nucleus GO:0042346 IMP
    cellular response to growth factor stimulus GO:0071363 IEA
    response to ATP GO:0033198 IEA
Subcellular Localization
    axon GO:0030424 IEA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IEA
    plasma membrane GO:0005886 IDA
    synaptic vesicle GO:0008021 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SPHK1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SPHK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAF2 7186
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 RPS6KC1  
Affinity Capture-Western Homo sapiens
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