Gene description for MAP1S
Gene name microtubule-associated protein 1S
Gene symbol MAP1S
Other names/aliases BPY2IP1
C19orf5
MAP8
VCY2IP-1
VCY2IP1
Species Homo sapiens
 Database cross references - MAP1S
ExoCarta ExoCarta_55201
Vesiclepedia VP_55201
Entrez Gene 55201
HGNC 15715
MIM 607573
UniProt Q66K74  
 MAP1S identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for MAP1S
Molecular Function
    DNA binding GO:0003677 IDA
    actin binding GO:0003779 IBA
    DNA nuclease activity GO:0004536 IDA
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IDA
    microtubule binding GO:0008017 TAS
    tubulin binding GO:0015631 IDA
    identical protein binding GO:0042802 IEA
    beta-tubulin binding GO:0048487 IDA
    actin filament binding GO:0051015 IDA
    actin filament binding GO:0051015 IDA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    microtubule bundle formation GO:0001578 IMP
    autophagy GO:0006914 TAS
    apoptotic process GO:0006915 IEA
    mitotic spindle organization GO:0007052 IMP
    nervous system development GO:0007399 ISS
    axonogenesis GO:0007409 IBA
    brain development GO:0007420 ISS
    dendrite development GO:0016358 IBA
    regulation of microtubule depolymerization GO:0031114 IBA
    microtubule anchoring at centrosome GO:0034454 IMP
    mitochondrion transport along microtubule GO:0047497 TAS
    neuron projection morphogenesis GO:0048812 IEP
    metaphase chromosome alignment GO:0051310 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    centrosome GO:0005813 IDA
    microtubule organizing center GO:0005815 IDA
    spindle GO:0005819 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IDA
    microtubule associated complex GO:0005875 IBA
    cell junction GO:0030054 IDA
    dendrite GO:0030425 IBA
    dendrite GO:0030425 ISS
    cell projection GO:0042995 IDA
    neuronal cell body GO:0043025 IBA
    neuronal cell body GO:0043025 ISS
    synapse GO:0045202 IBA
    synapse GO:0045202 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    mitotic spindle microtubule GO:1990498 IDA
 Experiment description of studies that identified MAP1S in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAP1S
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KIF2A 3796
Proximity Label-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 EDEM1  
Affinity Capture-MS Homo sapiens
5 DBF4B 80174
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GSN 2934
Affinity Capture-MS Homo sapiens
7 RASSF3 283349
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HDAC4  
Two-hybrid Homo sapiens
9 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ANXA2 302
Affinity Capture-MS Homo sapiens
12 H2BFWT  
Affinity Capture-MS Homo sapiens
13 GPR45  
Affinity Capture-MS Homo sapiens
14 KIF3A 11127
Affinity Capture-MS Homo sapiens
15 VAPB 9217
Affinity Capture-MS Homo sapiens
16 DHDH  
Affinity Capture-MS Homo sapiens
17 SPHK1 8877
Affinity Capture-MS Homo sapiens
18 LIPH 200879
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MAP1B 4131
Affinity Capture-MS Homo sapiens
20 FAS 355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RABEP2 79874
Affinity Capture-MS Homo sapiens
22 PIN1 5300
Reconstituted Complex Homo sapiens
23 CTAG1B  
Affinity Capture-MS Homo sapiens
24 AURKA 6790
Affinity Capture-MS Homo sapiens
25 NLGN3  
Two-hybrid Homo sapiens
26 ANXA1 301
Affinity Capture-MS Homo sapiens
27 C2orf27B  
Affinity Capture-MS Homo sapiens
28 SULT1C4  
Affinity Capture-MS Homo sapiens
29 CNPY2 10330
Co-fractionation Homo sapiens
30 STIP1 10963
Affinity Capture-MS Homo sapiens
31 C9orf78 51759
Affinity Capture-MS Homo sapiens
32 RASSF1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
33 CPNE9  
Affinity Capture-MS Homo sapiens
34 SOCS3 9021
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
35 VHL  
Two-hybrid Homo sapiens
36 LOC100132735  
Protein-RNA Homo sapiens
37 CAMSAP2  
Proximity Label-MS Homo sapiens
38 ELMOD3  
Affinity Capture-MS Homo sapiens
39 KLHL34  
Affinity Capture-MS Homo sapiens
40 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
41 STK3 6788
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 WIF1 11197
Affinity Capture-MS Homo sapiens
43 MAPRE1 22919
Proximity Label-MS Homo sapiens
44 TRAPPC1 58485
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 Rassf1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
46 C6orf136  
Affinity Capture-MS Homo sapiens
47 LRPPRC 10128
Two-hybrid Homo sapiens
48 LRRC27  
Affinity Capture-MS Homo sapiens
49 BPY2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
50 MAPRE3  
Proximity Label-MS Homo sapiens
51 MAP1LC3A 84557
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
52 NOXO1  
Affinity Capture-MS Homo sapiens
53 PADI4  
Affinity Capture-MS Homo sapiens
54 HDHD1  
Affinity Capture-MS Homo sapiens
55 STRN 6801
Affinity Capture-MS Homo sapiens
56 HECW2  
Affinity Capture-MS Homo sapiens
57 NTRK1 4914
Affinity Capture-MS Homo sapiens
58 MND1  
Affinity Capture-MS Homo sapiens
59 B2M 567
Affinity Capture-MS Homo sapiens
60 SYNCRIP 10492
Co-fractionation Homo sapiens
61 FAM174A 345757
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 DCTN1 1639
Proximity Label-MS Homo sapiens
63 PTGES3 10728
Affinity Capture-MS Homo sapiens
64 FKBP3 2287
Co-fractionation Homo sapiens
65 PML 5371
Affinity Capture-MS Homo sapiens
66 SF3B3 23450
Affinity Capture-MS Homo sapiens
67 STK4 6789
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 CTNNA3  
Affinity Capture-MS Homo sapiens
69 CAPN10  
Affinity Capture-MS Homo sapiens
70 RBBP5 5929
Affinity Capture-MS Homo sapiens
71 RPA3 6119
Proximity Label-MS Homo sapiens
72 GGA1 26088
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 PPM1G 5496
Affinity Capture-MS Homo sapiens
74 C9orf72  
Affinity Capture-MS Homo sapiens
75 RASSF5 83593
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
76 ATG3 64422
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 UBXN6 80700
Affinity Capture-MS Homo sapiens
78 HNRNPA2B1 3181
Co-fractionation Homo sapiens
79 ATG7 10533
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MAP1S is involved
No pathways found





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