Experiment description of studies that identified RASSF5 in exosomes

1 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

Protein-protein interactions for RASSF5

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RAP2B 5912 Two-hybrid Homo sapiens 2 MOB1A 55233 Proximity Label-MS Homo sapiens 3 STK3 6788 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 BTRC 8945 Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 5 MYLIP   Two-hybrid Homo sapiens 6 TRAF2 7186 Two-hybrid Homo sapiens 7 MOB1B 92597 Proximity Label-MS Homo sapiens 8 VPS28 51160 Two-hybrid Homo sapiens 9 MAP1S 55201 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 RASSF2 9770 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 11 PFN1 5216 Proximity Label-MS Homo sapiens 12 AMOTL2 51421 Two-hybrid Homo sapiens 13 REL 5966 Two-hybrid Homo sapiens 14 KCTD12 115207 Affinity Capture-MS Homo sapiens 15 TNFRSF1A 7132 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 16 HRAS 3265 Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens 17 RASSF1   Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 18 SMAD4   Two-hybrid Homo sapiens 19 MAP1B 4131 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 HIPK1 204851 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 21 AGL 178 Affinity Capture-MS Homo sapiens 22 MRAS 22808 Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 23 KRTAP4-2   Two-hybrid Homo sapiens 24 ERP44 23071 Affinity Capture-MS Homo sapiens 25 APPBP2   Two-hybrid Homo sapiens 26 MAP1LC3B 81631 Affinity Capture-MS Homo sapiens 27 LSM7   Two-hybrid Homo sapiens 28 MAP1LC3A 84557 Affinity Capture-MS Homo sapiens 29 RRAS 6237 Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 30 RAP1B 5908 Two-hybrid Homo sapiens 31 SAV1   Affinity Capture-MS Homo sapiens 32 UBQLN2 29978 Two-hybrid Homo sapiens 33 FLNC 2318 Affinity Capture-MS Homo sapiens 34 KRT40   Two-hybrid Homo sapiens 35 KRAS 3845 Reconstituted Complex Homo sapiens 36 STK4 6789 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 37 RABIF   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 38 UBQLN1 29979 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 39 ITCH 83737 Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 40 MDM2   Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 41 RASSF5 83593 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 42 ADH6 130 Two-hybrid Homo sapiens 43 TRAF1 7185 Two-hybrid Homo sapiens 44 RAP2A 5911 Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens View the network

Pathways in which RASSF5 is involved

No pathways found