Gene description for HIPK1
Gene name homeodomain interacting protein kinase 1
Gene symbol HIPK1
Other names/aliases Myak
Nbak2
Species Homo sapiens
 Database cross references - HIPK1
ExoCarta ExoCarta_204851
Vesiclepedia VP_204851
Entrez Gene 204851
HGNC 19006
MIM 608003
UniProt Q86Z02  
 HIPK1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for HIPK1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein tyrosine kinase activity GO:0004713 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    eye development GO:0001654 ISS
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    smoothened signaling pathway GO:0007224 IBA
    cell population proliferation GO:0008283 IEA
    positive regulation of cell population proliferation GO:0008284 IEA
    anterior/posterior pattern specification GO:0009952 IEA
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 IDA
    retina layer formation GO:0010842 IEA
    neuron differentiation GO:0030182 IEA
    adherens junction assembly GO:0034333 IEA
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IBA
    positive regulation of angiogenesis GO:0045766 ISS
    embryonic camera-type eye morphogenesis GO:0048596 IEA
    embryonic retina morphogenesis in camera-type eye GO:0060059 IEA
    definitive hemopoiesis GO:0060216 ISS
    lens induction in camera-type eye GO:0060235 IEA
    iris morphogenesis GO:0061072 IEA
    endothelial cell apoptotic process GO:0072577 IDA
    extrinsic apoptotic signaling pathway GO:0097191 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    PML body GO:0016605 IBA
    nuclear speck GO:0016607 IEA
 Experiment description of studies that identified HIPK1 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for HIPK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Affinity Capture-Western Homo sapiens
2 A2M 2
Affinity Capture-MS Homo sapiens
3 ATP13A2  
Two-hybrid Homo sapiens
4 DDX54 79039
Affinity Capture-MS Homo sapiens
5 PML 5371
Affinity Capture-MS Homo sapiens
6 SENP1  
Biochemical Activity Homo sapiens
7 UBE2I 7329
Two-hybrid Homo sapiens
8 HAX1  
Affinity Capture-MS Homo sapiens
9 SHARPIN  
Affinity Capture-MS Homo sapiens
10 NEB 4703
Affinity Capture-MS Homo sapiens
11 FHL3 2275
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
12 GLUL 2752
Two-hybrid Homo sapiens
13 SKIL  
Two-hybrid Homo sapiens
14 FOXP3  
Affinity Capture-MS Homo sapiens
15 FHL2 2274
Affinity Capture-MS Homo sapiens
16 TP53 7157
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
17 RASSF5 83593
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 DAXX  
Affinity Capture-Western Homo sapiens
19 PRPF40A 55660
Two-hybrid Homo sapiens
20 PARK7 11315
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 CIT 11113
Affinity Capture-MS Homo sapiens
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