Gene description for PARK7
Gene name parkinson protein 7
Gene symbol PARK7
Other names/aliases DJ-1
DJ1
HEL-S-67p
Species Homo sapiens
 Database cross references - PARK7
ExoCarta ExoCarta_11315
Vesiclepedia VP_11315
Entrez Gene 11315
HGNC 16369
MIM 602533
UniProt Q99497  
 PARK7 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 35918900    
Breast cancer cells 35918900    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
Saliva 19199708    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
Thymus 23844026    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for PARK7
Molecular Function
    core promoter sequence-specific DNA binding GO:0001046 IC
    double-stranded DNA binding GO:0003690 IDA
    single-stranded DNA binding GO:0003697 IDA
    transcription coactivator activity GO:0003713 IGI
    transcription coactivator activity GO:0003713 TAS
    mRNA binding GO:0003729 IDA
    signaling receptor binding GO:0005102 IPI
    copper ion binding GO:0005507 IDA
    protein binding GO:0005515 IPI
    enzyme activator activity GO:0008047 IDA
    peptidase activity GO:0008233 IDA
    superoxide dismutase copper chaperone activity GO:0016532 IDA
    oxidoreductase activity, acting on peroxide as acceptor GO:0016684 IBA
    oxidoreductase activity, acting on peroxide as acceptor GO:0016684 IDA
    oxygen sensor activity GO:0019826 IEA
    enzyme binding GO:0019899 IPI
    kinase binding GO:0019900 IPI
    cytokine binding GO:0019955 IPI
    peptidase inhibitor activity GO:0030414 IDA
    signaling receptor activator activity GO:0030546 IDA
    tyrosine 3-monooxygenase activator activity GO:0036470 IDA
    L-dopa decarboxylase activator activity GO:0036478 IDA
    protein deglycase activity GO:0036524 IDA
    protein deglycase activity GO:0036524 IDA
    protein deglycase activity GO:0036524 IMP
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    protein homodimerization activity GO:0042803 IPI
    small protein activating enzyme binding GO:0044388 IPI
    ubiquitin-like protein conjugating enzyme binding GO:0044390 IPI
    cadherin binding GO:0045296 HDA
    mercury ion binding GO:0045340 IDA
    nuclear androgen receptor binding GO:0050681 IPI
    peroxiredoxin activity GO:0051920 IEA
    ubiquitin-protein transferase inhibitor activity GO:0055105 IDA
    scaffold protein binding GO:0097110 IPI
    DNA-binding transcription factor binding GO:0140297 IPI
    cupric ion binding GO:1903135 IDA
    cuprous ion binding GO:1903136 IDA
    ubiquitin-specific protease binding GO:1990381 IPI
    glyoxalase (glycolic acid-forming) activity GO:1990422 IDA
Biological Process
    negative regulation of protein phosphorylation GO:0001933 IGI
    positive regulation of acute inflammatory response to antigenic stimulus GO:0002866 ISS
    DNA repair GO:0006281 IDA
    proteolysis GO:0006508 IEA
    autophagy GO:0006914 IEA
    inflammatory response GO:0006954 IEA
    response to oxidative stress GO:0006979 IBA
    mitochondrion organization GO:0007005 ISS
    Ras protein signal transduction GO:0007265 TAS
    single fertilization GO:0007338 IEA
    adult locomotory behavior GO:0008344 IEA
    methylglyoxal metabolic process GO:0009438 IDA
    methylglyoxal metabolic process GO:0009438 IDA
    detoxification of copper ion GO:0010273 IMP
    positive regulation of gene expression GO:0010628 TAS
    negative regulation of gene expression GO:0010629 IDA
    lactate biosynthetic process GO:0019249 IDA
    removal of superoxide radicals GO:0019430 IDA
    insulin secretion GO:0030073 ISS
    protein repair GO:0030091 IDA
    androgen receptor signaling pathway GO:0030521 IMP
    positive regulation of protein-containing complex assembly GO:0031334 IDA
    negative regulation of protein ubiquitination GO:0031397 IDA
    negative regulation of protein ubiquitination GO:0031397 IDA
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IDA
    regulation of TRAIL production GO:0032679 IMP
    positive regulation of interleukin-8 production GO:0032757 IDA
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IMP
    negative regulation of protein sumoylation GO:0033234 IDA
    cellular response to oxidative stress GO:0034599 IDA
    cellular response to oxidative stress GO:0034599 IMP
    cellular response to glyoxal GO:0036471 IDA
    glucose homeostasis GO:0042593 ISS
    hydrogen peroxide metabolic process GO:0042743 IDA
    regulation of neuron apoptotic process GO:0043523 IDA
    negative regulation of neuron apoptotic process GO:0043524 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    glycolate biosynthetic process GO:0046295 IBA
    glycolate biosynthetic process GO:0046295 IDA
    negative regulation of protein export from nucleus GO:0046826 IGI
    regulation of inflammatory response GO:0050727 ISS
    detoxification of mercury ion GO:0050787 IEA
    protein stabilization GO:0050821 IDA
    protein stabilization GO:0050821 IMP
    protein stabilization GO:0050821 IMP
    dopamine uptake involved in synaptic transmission GO:0051583 IEA
    regulation of mitochondrial membrane potential GO:0051881 IMP
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IC
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IMP
    membrane depolarization GO:0051899 IEA
    membrane hyperpolarization GO:0060081 IEA
    regulation of androgen receptor signaling pathway GO:0060765 IDA
    detoxification of hydrogen peroxide GO:0061691 IDA
    methylglyoxal catabolic process to lactate GO:0061727 IDA
    cellular response to hydrogen peroxide GO:0070301 IMP
    detection of oxidative stress GO:0070994 IEA
    guanine deglycation GO:0106044 IDA
    guanine deglycation, methylglyoxal removal GO:0106045 IDA
    guanine deglycation, glyoxal removal GO:0106046 IDA
    cellular detoxification of aldehyde GO:0110095 IDA
    cellular detoxification of methylglyoxal GO:0140041 IDA
    positive regulation of protein localization to nucleus GO:1900182 IDA
    positive regulation of protein localization to nucleus GO:1900182 IMP
    regulation of synaptic vesicle endocytosis GO:1900242 IEA
    negative regulation of protein acetylation GO:1901984 IDA
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 IDA
    positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902177 IEA
    negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902236 IGI
    regulation of supramolecular fiber organization GO:1902903 TAS
    positive regulation of mitochondrial electron transport, NADH to ubiquinone GO:1902958 IMP
    negative regulation of death-inducing signaling complex assembly GO:1903073 IMP
    negative regulation of protein K48-linked deubiquitination GO:1903094 IDA
    negative regulation of TRAIL-activated apoptotic signaling pathway GO:1903122 IMP
    positive regulation of dopamine biosynthetic process GO:1903181 IC
    positive regulation of dopamine biosynthetic process GO:1903181 IDA
    glyoxal metabolic process GO:1903189 IBA
    glyoxal metabolic process GO:1903189 IDA
    glyoxal catabolic process GO:1903190 IEA
    positive regulation of L-dopa biosynthetic process GO:1903197 IMP
    regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway GO:1903376 IDA
    negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway GO:1903377 IDA
    negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway GO:1903377 IMP
    negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway GO:1903384 IGI
    negative regulation of reactive oxygen species biosynthetic process GO:1903427 ISS
    positive regulation of reactive oxygen species biosynthetic process GO:1903428 IEA
    positive regulation of autophagy of mitochondrion GO:1903599 NAS
    negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide GO:1903751 IDA
    negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway GO:1905259 IDA
    positive regulation of reactive oxygen species metabolic process GO:2000379 IDA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IMP
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IMP
    mitochondrial intermembrane space GO:0005758 IEA
    mitochondrial matrix GO:0005759 IEA
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IMP
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    adherens junction GO:0005912 HDA
    synaptic vesicle GO:0008021 IEA
    PML body GO:0016605 IDA
    axon GO:0030424 ISS
    cell body GO:0044297 IEA
    membrane raft GO:0045121 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PARK7 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
9
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 1334
MISEV standards
Biophysical techniques
✔
CD9
Enriched markers
✔
ACTB
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name LM2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
13
Experiment ID 1336
MISEV standards
Biophysical techniques
✔
CD9
Enriched markers
✔
ACTB
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name LM2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
14
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
15
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
16
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
17
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
18
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
29
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
30
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
31
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
34
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
35
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
36
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 219
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name C666-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
38
Experiment ID 220
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP69
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
39
Experiment ID 221
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP460
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
40
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
41
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
42
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
43
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
44
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
45
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
46
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
47
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
48
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
49
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
50
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
51
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
52
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
53
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
54
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
55
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
56
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
57
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
58
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
59
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PARK7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MPST 4357
Co-fractionation Homo sapiens
2 GSTP1 2950
Co-fractionation Homo sapiens
3 GLRX5 51218
Co-fractionation Homo sapiens
4 LDHA 3939
Co-fractionation Homo sapiens
5 UBE2H 7328
Affinity Capture-MS Homo sapiens
6 C1orf198 84886
Co-fractionation Homo sapiens
7 ISG15 9636
Co-fractionation Homo sapiens
8 GATAD2B 57459
Affinity Capture-MS Homo sapiens
9 ATPIF1 93974
Affinity Capture-MS Homo sapiens
10 HDAC2 3066
Affinity Capture-MS Homo sapiens
11 PTMS 5763
Affinity Capture-MS Homo sapiens
12 RAB1A 5861
Affinity Capture-MS Homo sapiens
13 ANP32A 8125
Affinity Capture-MS Homo sapiens
14 DDAH1 23576
Co-fractionation Homo sapiens
15 LDHB 3945
Co-fractionation Homo sapiens
16 PKM 5315
Co-fractionation Homo sapiens
17 DNM1L 10059
Affinity Capture-MS Homo sapiens
18 RPA2 6118
Proximity Label-MS Homo sapiens
19 NACA 4666
Affinity Capture-MS Homo sapiens
20 THRAP3 9967
Affinity Capture-MS Homo sapiens
21 ACTC1 70
Affinity Capture-MS Homo sapiens
22 UCHL1 7345
Co-fractionation Homo sapiens
23 CMPK1 51727
Co-fractionation Homo sapiens
24 LIMA1 51474
Affinity Capture-MS Homo sapiens
25 PHOSPHO1  
Affinity Capture-MS Homo sapiens
26 TAB1 10454
Affinity Capture-MS Homo sapiens
27 ARCN1 372
Co-fractionation Homo sapiens
28 PPIA 5478
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
29 VHL  
Affinity Capture-Western Homo sapiens
30 NDUFA4 4697
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
31 PIR 8544
Co-fractionation Homo sapiens
32 CFL1 1072
Co-fractionation Homo sapiens
33 PARK7 11315
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
FRET Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
FRET Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
34 SOX2  
Affinity Capture-MS Homo sapiens
35 DUSP28  
Affinity Capture-MS Homo sapiens
36 SFPQ 6421
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
37 ILF2 3608
Affinity Capture-MS Homo sapiens
38 APEX1 328
Affinity Capture-RNA Homo sapiens
39 PPTC7 160760
Affinity Capture-MS Homo sapiens
40 DUSP12 11266
Affinity Capture-MS Homo sapiens
41 PARK2  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
42 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
43 PGK1 5230
Affinity Capture-MS Homo sapiens
44 NDUFS3 4722
Affinity Capture-Western Homo sapiens
45 CRYZ 1429
Co-fractionation Homo sapiens
46 DSTN 11034
Co-fractionation Homo sapiens
47 TPM3 7170
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 MCM2 4171
Affinity Capture-MS Homo sapiens
49 HINT2 84681
Co-fractionation Homo sapiens
50 PDHB 5162
Affinity Capture-MS Homo sapiens
51 STMN1 3925
Affinity Capture-MS Homo sapiens
52 ANP32B 10541
Affinity Capture-MS Homo sapiens
53 IMPDH2 3615
Affinity Capture-MS Homo sapiens
54 AK3 50808
Co-fractionation Homo sapiens
55 PGM1 5236
Co-fractionation Homo sapiens
56 TDP2 51567
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
57 DUSP2  
Affinity Capture-MS Homo sapiens
58 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
59 BASP1 10409
Affinity Capture-MS Homo sapiens
60 DDX58 23586
Affinity Capture-RNA Homo sapiens
61 HMGA1 3159
Affinity Capture-MS Homo sapiens
62 HADHA 3030
Affinity Capture-MS Homo sapiens
63 OTUD7B 56957
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
64 GOPC 57120
Two-hybrid Homo sapiens
65 Ubr5  
Affinity Capture-MS Mus musculus
66 EEF1G 1937
Affinity Capture-MS Homo sapiens
67 TMOD3 29766
Affinity Capture-MS Homo sapiens
68 NDUFAB1 4706
Co-fractionation Homo sapiens
69 CHTOP  
Affinity Capture-MS Homo sapiens
70 BOLA2B 654483
Affinity Capture-MS Homo sapiens
71 CALR 811
Affinity Capture-MS Homo sapiens
72 ARIH2 10425
Affinity Capture-MS Homo sapiens
73 NEDD4 4734
Affinity Capture-MS Homo sapiens
74 SOD1 6647
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
PCA Homo sapiens
75 MTA2 9219
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
76 MSRB2  
Co-fractionation Homo sapiens
77 YWHAB 7529
Affinity Capture-MS Homo sapiens
78 MDH2 4191
Affinity Capture-MS Homo sapiens
79 UNK  
Affinity Capture-RNA Homo sapiens
80 PFN1 5216
Affinity Capture-MS Homo sapiens
81 PPM1D  
Affinity Capture-MS Homo sapiens
82 MARCKS 4082
Affinity Capture-MS Homo sapiens
83 PRDX2 7001
Co-fractionation Homo sapiens
84 DLD 1738
Affinity Capture-MS Homo sapiens
85 PPIB 5479
Affinity Capture-MS Homo sapiens
86 PRDX5 25824
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
87 THEM4  
Co-fractionation Homo sapiens
88 RIN3  
Affinity Capture-MS Homo sapiens
89 PITX3  
Co-localization Homo sapiens
90 IMPA2 3613
Co-fractionation Homo sapiens
91 MAP1B 4131
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
92 COPE 11316
Affinity Capture-MS Homo sapiens
93 ADA 100
Co-fractionation Homo sapiens
94 NONO 4841
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
95 TKT 7086
Affinity Capture-MS Homo sapiens
96 TRIM24  
Affinity Capture-MS Homo sapiens
97 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
98 UBE2I 7329
Two-hybrid Homo sapiens
99 PABPC1 26986
Affinity Capture-MS Homo sapiens
100 CDH1 999
Proximity Label-MS Homo sapiens
101 CHMP4B 128866
Affinity Capture-MS Homo sapiens
102 SREBF2 6721
Affinity Capture-Western Homo sapiens
103 BBS1 582
Affinity Capture-MS Homo sapiens
104 VCP 7415
Affinity Capture-MS Homo sapiens
105 HMGN1  
Affinity Capture-MS Homo sapiens
106 SLX4  
Affinity Capture-MS Homo sapiens
107 SPR 6697
Co-fractionation Homo sapiens
108 TPI1 7167
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
109 U2AF2 11338
Affinity Capture-MS Homo sapiens
110 PDIA6 10130
Affinity Capture-MS Homo sapiens
111 SUMO2 6613
Affinity Capture-MS Homo sapiens
112 POLDIP2 26073
Co-fractionation Homo sapiens
113 NOS2  
Co-fractionation Homo sapiens
114 YWHAQ 10971
Affinity Capture-MS Homo sapiens
115 ENO1 2023
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
116 ALYREF 10189
Affinity Capture-MS Homo sapiens
117 MYH11 4629
Affinity Capture-MS Homo sapiens
118 HRNR 388697
Affinity Capture-MS Homo sapiens
119 RAB11FIP5 26056
Co-fractionation Homo sapiens
120 MBD3 53615
Affinity Capture-MS Homo sapiens
121 LYPLA1 10434
Co-fractionation Homo sapiens
122 EPB41L5 57669
Affinity Capture-MS Homo sapiens
123 DDX39B 7919
Affinity Capture-MS Homo sapiens
124 CLIC4 25932
Co-fractionation Homo sapiens
125 MYL12A 10627
Affinity Capture-MS Homo sapiens
126 FAHD2A 51011
Co-fractionation Homo sapiens
127 DNAJB11 51726
Proximity Label-MS Homo sapiens
128 DDAH2 23564
Co-fractionation Homo sapiens
129 BCLAF1 9774
Affinity Capture-MS Homo sapiens
130 ND1  
Affinity Capture-Western Homo sapiens
131 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
132 PPM1B 5495
Affinity Capture-MS Homo sapiens
133 ACTN1 87
Affinity Capture-MS Homo sapiens
134 PRDX6 9588
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
135 DUSP1  
Affinity Capture-MS Homo sapiens
136 PGLS 25796
Co-fractionation Homo sapiens
137 BAX 581
Reconstituted Complex Homo sapiens
138 ASS1 445
Co-fractionation Homo sapiens
139 YWHAZ 7534
Affinity Capture-MS Homo sapiens
140 EIF6 3692
Affinity Capture-MS Homo sapiens
141 SAR1B 51128
Affinity Capture-MS Homo sapiens
142 ECT2 1894
Affinity Capture-MS Homo sapiens
143 PNN 5411
Affinity Capture-MS Homo sapiens
144 PIAS2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
145 CTNNB1 1499
Affinity Capture-MS Homo sapiens
146 GCSH 2653
Co-fractionation Homo sapiens
147 ANXA5 308
Affinity Capture-MS Homo sapiens
148 CD274 29126
Affinity Capture-MS Homo sapiens
149 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
150 CUL3 8452
Affinity Capture-MS Homo sapiens
151 PTEN 5728
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
152 HSD17B10 3028
Affinity Capture-MS Homo sapiens
153 NENF 29937
Co-fractionation Homo sapiens
154 HDHD3 81932
Co-fractionation Homo sapiens
155 CALM1 801
Affinity Capture-MS Homo sapiens
156 DUSP6 1848
Affinity Capture-MS Homo sapiens
157 NXF1 10482
Affinity Capture-RNA Homo sapiens
158 HIST1H2AB 8335
Affinity Capture-MS Homo sapiens
159 PRKACA 5566
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
160 TPM1 7168
Affinity Capture-MS Homo sapiens
161 UBA2 10054
Two-hybrid Homo sapiens
162 DKC1 1736
Affinity Capture-MS Homo sapiens
163 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
164 BAG1 573
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
165 SLC18A2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
166 ACO2 50
Co-fractionation Homo sapiens
167 TMOD2 29767
Affinity Capture-MS Homo sapiens
168 LACTB2 51110
Co-fractionation Homo sapiens
169 MAPKAPK5 8550
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
170 PEBP1 5037
Affinity Capture-MS Homo sapiens
171 USP19 10869
Affinity Capture-MS Homo sapiens
172 CFL2 1073
Co-fractionation Homo sapiens
173 HSPA4 3308
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
174 DUSP9  
Affinity Capture-MS Homo sapiens
175 DNAJC3 5611
Proximity Label-MS Homo sapiens
176 CLCF1  
Affinity Capture-MS Homo sapiens
177 FKBP3 2287
Affinity Capture-MS Homo sapiens
178 DDRGK1 65992
Affinity Capture-MS Homo sapiens
179 HIPK1 204851
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
180 TRIM28 10155
Affinity Capture-MS Homo sapiens
181 PGAM1 5223
Affinity Capture-MS Homo sapiens
182 C11orf84  
Affinity Capture-MS Homo sapiens
183 EIF3B 8662
Affinity Capture-MS Homo sapiens
184 PDP1 54704
Co-fractionation Homo sapiens
185 HINT1 3094
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
186 HNRNPA1L2 144983
Affinity Capture-MS Homo sapiens
187 HMGB1P1 10357
Affinity Capture-MS Homo sapiens
188 RBBP4 5928
Affinity Capture-MS Homo sapiens
189 GEMIN4 50628
Affinity Capture-MS Homo sapiens
190 SUMO1 7341
Two-hybrid Homo sapiens
191 Bax 12028
Affinity Capture-Western Mus musculus
192 STUB1 10273
Affinity Capture-Western Homo sapiens
193 NIT2 56954
Co-fractionation Homo sapiens
194 DUSP19  
Affinity Capture-MS Homo sapiens
195 SNCA 6622
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
196 MYCBP 26292
Affinity Capture-MS Homo sapiens
197 FADD 8772
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
198 PRKCSH 5589
Affinity Capture-MS Homo sapiens
199 CLIC1 1192
Co-fractionation Homo sapiens
200 CCT8 10694
Affinity Capture-MS Homo sapiens
201 SOD2 6648
Co-fractionation Homo sapiens
202 ATP5J 522
Co-fractionation Homo sapiens
203 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
204 MTRF1  
Affinity Capture-MS Homo sapiens
205 GPI 2821
Co-fractionation Homo sapiens
206 MTA1 9112
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
207 PDIA4 9601
Affinity Capture-MS Homo sapiens
208 WBP11  
Affinity Capture-MS Homo sapiens
209 TALDO1 6888
Co-fractionation Homo sapiens
210 ISOC2  
Co-fractionation Homo sapiens
211 DBN1 1627
Affinity Capture-MS Homo sapiens
212 LGALS3 3958
Co-fractionation Homo sapiens
213 SAP18 10284
Affinity Capture-MS Homo sapiens
214 GRHPR 9380
Co-fractionation Homo sapiens
215 ACAA2 10449
Co-fractionation Homo sapiens
216 PDHA1 5160
Affinity Capture-MS Homo sapiens
217 CASP8 841
Reconstituted Complex Homo sapiens
218 NHLRC2 374354
Affinity Capture-MS Homo sapiens
219 ZBTB2 57621
Affinity Capture-MS Homo sapiens
220 HDAC1 3065
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
221 MAP3K5 4217
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
222 MTHFD1 4522
Affinity Capture-MS Homo sapiens
223 C21orf33  
Co-fractionation Homo sapiens
224 BOLA2 552900
Affinity Capture-MS Homo sapiens
225 SSBP1 6742
Affinity Capture-MS Homo sapiens
226 OAT 4942
Affinity Capture-MS Homo sapiens
227 TRAP1 10131
Affinity Capture-MS Homo sapiens
228 EEF2 1938
Affinity Capture-MS Homo sapiens
229 BABAM1 29086
Affinity Capture-MS Homo sapiens
230 MIF 4282
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
231 TXN 7295
Affinity Capture-MS Homo sapiens
232 IDH2 3418
Co-fractionation Homo sapiens
233 SEC14L2  
Co-fractionation Homo sapiens
234 NQO1 1728
Co-fractionation Homo sapiens
235 MRPS18B 28973
Affinity Capture-MS Homo sapiens
236 BCL2L1 598
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
237 CSN1S1 1446
Biochemical Activity Homo sapiens
238 EFCAB6  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
239 ACOT1 641371
Co-fractionation Homo sapiens
240 ITPA 3704
Co-fractionation Homo sapiens
241 PINK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
242 RNPS1 10921
Affinity Capture-MS Homo sapiens
243 RBM8A 9939
Affinity Capture-MS Homo sapiens
244 DAXX  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
245 ARF1 375
Affinity Capture-MS Homo sapiens
246 DUSP4 1846
Affinity Capture-MS Homo sapiens
247 SERBP1 26135
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
248 PRPF4 9128
Affinity Capture-MS Homo sapiens
249 C9orf72  
Affinity Capture-MS Homo sapiens
250 AR 367
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
251 ANXA7 310
Co-fractionation Homo sapiens
252 PPARGC1A  
Phenotypic Enhancement Homo sapiens
253 PRDX3 10935
Affinity Capture-MS Homo sapiens
254 CPSF6 11052
Affinity Capture-MS Homo sapiens
255 CHD4 1108
Affinity Capture-MS Homo sapiens
256 HIST1H2AE 3012
Affinity Capture-MS Homo sapiens
257 MRPS9 64965
Affinity Capture-MS Homo sapiens
258 RBBP7 5931
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here