Gene description for UBA2
Gene name ubiquitin-like modifier activating enzyme 2
Gene symbol UBA2
Other names/aliases ARX
HRIHFB2115
SAE2
Species Homo sapiens
 Database cross references - UBA2
ExoCarta ExoCarta_10054
Entrez Gene 10054
HGNC 30661
MIM 613295
UniProt Q9UBT2  
 UBA2 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for UBA2
Molecular Function
    transcription factor binding GO:0008134 IEA
    ATP binding GO:0005524 IEA
    enzyme activator activity GO:0008047 TAS
    protein heterodimerization activity GO:0046982 IPI
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    SUMO activating enzyme activity GO:0019948 TAS
Biological Process
    positive regulation of catalytic activity GO:0043085 TAS
    protein sumoylation GO:0016925 TAS
    post-translational protein modification GO:0043687 TAS
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IBA
    SUMO activating enzyme complex GO:0031510 IDA
 Experiment description of studies that identified UBA2 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UBA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRIX1 55299
Two-hybrid Homo sapiens
2 SUMO3 6612
Affinity Capture-MS Homo sapiens
3 UBE2I 7329
Affinity Capture-MS Homo sapiens
4 SAE1 10055
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 NEDD8 4738
Invitro Homo sapiens
6 SUMO1 7341
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which UBA2 is involved
PathwayEvidenceSource
SUMO is conjugated to E1 (UBA2:SAE1) TAS Reactome
SUMO is transferred from E1 to E2 (UBE2I, UBC9) TAS Reactome





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