Gene description for IREB2
Gene name iron-responsive element binding protein 2
Gene symbol IREB2
Other names/aliases ACO3
IRP2
IRP2AD
Species Homo sapiens
 Database cross references - IREB2
ExoCarta ExoCarta_3658
Vesiclepedia VP_3658
Entrez Gene 3658
HGNC 6115
MIM 147582
UniProt P48200  
 IREB2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for IREB2
Molecular Function
    mRNA regulatory element binding translation repressor activity GO:0000900 EXP
    RNA binding GO:0003723 IDA
    RNA binding GO:0003723 TAS
    aconitate hydratase activity GO:0003994 IBA
    protein binding GO:0005515 IPI
    iron-responsive element binding GO:0030350 IBA
    iron-responsive element binding GO:0030350 IMP
    iron-responsive element binding GO:0030350 TAS
    metal ion binding GO:0046872 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 IBA
Biological Process
    tricarboxylic acid cycle GO:0006099 IBA
    citrate metabolic process GO:0006101 IBA
    protoporphyrinogen IX biosynthetic process GO:0006782 IEA
    intracellular iron ion homeostasis GO:0006879 IMP
    intracellular iron ion homeostasis GO:0006879 TAS
    post-embryonic development GO:0009791 IEA
    negative regulation of translation GO:0017148 IEA
    osteoclast differentiation GO:0030316 IEA
    erythrocyte homeostasis GO:0034101 IEA
    mRNA stabilization GO:0048255 TAS
    intestinal absorption GO:0050892 IEA
    multicellular organismal-level iron ion homeostasis GO:0060586 IEA
Subcellular Localization
    cytoplasm GO:0005737 NAS
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified IREB2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IREB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Co-fractionation Homo sapiens
2 TERT  
Two-hybrid Homo sapiens
3 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 YBX1 4904
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 NCOA6  
Co-fractionation Homo sapiens
6 MAP2K2 5605
Co-fractionation Homo sapiens
7 CAMKMT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 GAGE1  
Affinity Capture-MS Homo sapiens
9 PSMA3 5684
Affinity Capture-MS Homo sapiens
10 MEF2BNB  
Affinity Capture-MS Homo sapiens
11 OTUD3 23252
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 TFRC 7037
Affinity Capture-MS Homo sapiens
Affinity Capture-RNA Homo sapiens
13 CIAO1 9391
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 LIN28A  
Affinity Capture-MS Homo sapiens
15 FBXL5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 KCTD10 83892
Affinity Capture-MS Homo sapiens
17 APOBEC3D  
Affinity Capture-MS Homo sapiens
18 ARL15 54622
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 FAM96A  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ENSA 2029
Affinity Capture-MS Homo sapiens
21 SF1 7536
Co-fractionation Homo sapiens
22 NDUFS7 374291
Affinity Capture-MS Homo sapiens
23 VHL  
Affinity Capture-Western Homo sapiens
24 OTUB1 55611
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
25 SHMT1 6470
Co-fractionation Homo sapiens
26 Hdac6  
Affinity Capture-MS Mus musculus
27 APP 351
Reconstituted Complex Homo sapiens
28 FAM96B 51647
Affinity Capture-Western Homo sapiens
29 Cdc20  
Affinity Capture-MS Mus musculus
30 ZNF174  
Affinity Capture-MS Homo sapiens
31 CCNL2 81669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 UBA2 10054
Co-fractionation Homo sapiens
33 ALYREF 10189
Affinity Capture-MS Homo sapiens
34 GSPT2 23708
Affinity Capture-MS Homo sapiens
35 INSL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SRSF7 6432
Affinity Capture-MS Homo sapiens
37 PABPC5 140886
Affinity Capture-MS Homo sapiens
38 SLC9A3R1 9368
Co-fractionation Homo sapiens
39 RGS20 8601
Affinity Capture-MS Homo sapiens
40 ALDH3B1 221
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 HS1BP3 64342
Affinity Capture-MS Homo sapiens
42 LEMD3  
Co-fractionation Homo sapiens
43 USP53 54532
Two-hybrid Homo sapiens
44 RBCK1  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
45 CAMKK2 10645
Affinity Capture-MS Homo sapiens
46 MDM2  
Affinity Capture-RNA Homo sapiens
47 TNPO1 3842
Co-fractionation Homo sapiens
48 PAIP1 10605
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which IREB2 is involved
PathwayEvidenceSource
Iron uptake and transport TAS Reactome
Transport of small molecules TAS Reactome





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