Gene description for PAIP1
Gene name poly(A) binding protein interacting protein 1
Gene symbol PAIP1
Other names/aliases -
Species Homo sapiens
 Database cross references - PAIP1
ExoCarta ExoCarta_10605
Vesiclepedia VP_10605
Entrez Gene 10605
HGNC 16945
MIM 605184
UniProt Q9H074  
 PAIP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for PAIP1
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    translation activator activity GO:0008494 IBA
    translation activator activity GO:0008494 IDA
Biological Process
    translational initiation GO:0006413 TAS
    regulation of translational initiation GO:0006446 IBA
    viral translation GO:0019081 IDA
    positive regulation by host of viral process GO:0044794 IDA
    mRNA stabilization GO:0048255 TAS
    CRD-mediated mRNA stabilization GO:0070934 IDA
    negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900152 IDA
    positive regulation of cytoplasmic translation GO:2000767 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    mCRD-mediated mRNA stability complex GO:0106002 IPI
 Experiment description of studies that identified PAIP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PAIP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Reconstituted Complex Homo sapiens
2 HNRNPC 3183
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NCBP1 4686
Affinity Capture-MS Homo sapiens
4 PER1  
Affinity Capture-MS Homo sapiens
5 DAZL  
Affinity Capture-MS Homo sapiens
6 TRUB2  
Affinity Capture-MS Homo sapiens
7 LARP1B 55132
Affinity Capture-MS Homo sapiens
8 ZFR 51663
Affinity Capture-MS Homo sapiens
9 PUM2 23369
Affinity Capture-MS Homo sapiens
10 EIF4A1 1973
Affinity Capture-Western Homo sapiens
11 HNRNPCL1 343069
Affinity Capture-MS Homo sapiens
12 CAND1 55832
Affinity Capture-MS Homo sapiens
13 CCDC9  
Affinity Capture-MS Homo sapiens
14 ILF2 3608
Affinity Capture-MS Homo sapiens
15 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
16 GSPT2 23708
Affinity Capture-MS Homo sapiens
17 RNPS1 10921
Affinity Capture-MS Homo sapiens
18 VARS 7407
Co-fractionation Homo sapiens
19 PABPC4L 132430
Affinity Capture-MS Homo sapiens
20 EIF3B 8662
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 SUPV3L1 6832
Affinity Capture-MS Homo sapiens
22 NCOA5  
Affinity Capture-MS Homo sapiens
23 SRRM2 23524
Affinity Capture-MS Homo sapiens
24 GSPT1 2935
Affinity Capture-MS Homo sapiens
25 C17orf85  
Affinity Capture-MS Homo sapiens
26 ZCCHC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 STAU2 27067
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MYB  
Two-hybrid Homo sapiens
29 MYCN  
Affinity Capture-MS Homo sapiens
30 TMEM132D  
Two-hybrid Homo sapiens
31 SREK1 140890
Affinity Capture-MS Homo sapiens
32 PABPC4 8761
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 FAM120A 23196
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MTERF3  
Affinity Capture-MS Homo sapiens
35 DHX36 170506
Affinity Capture-MS Homo sapiens
36 LARP4B 23185
Affinity Capture-MS Homo sapiens
37 PRNP 5621
Affinity Capture-MS Homo sapiens
38 ADSL 158
Co-fractionation Homo sapiens
39 RBMX 27316
Affinity Capture-MS Homo sapiens
40 CDKN2AIP  
Affinity Capture-MS Homo sapiens
41 Ubr5  
Co-crystal Structure Rattus norvegicus
42 SRRT 51593
Affinity Capture-MS Homo sapiens
43 USP12 219333
Affinity Capture-MS Homo sapiens
44 PARK2  
Affinity Capture-MS Homo sapiens
45 TRA2A 29896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PTCD1 26024
Affinity Capture-MS Homo sapiens
47 TPD52 7163
Co-fractionation Homo sapiens
48 Pabpc1 18458
Affinity Capture-MS Mus musculus
49 UBR5 51366
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 PABPC1 26986
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MKRN2 23609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 OGT 8473
Reconstituted Complex Homo sapiens
53 SYNCRIP 10492
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
54 LUC7L2 51631
Affinity Capture-MS Homo sapiens
55 BICD2 23299
Proximity Label-MS Homo sapiens
56 PPM1G 5496
Co-fractionation Homo sapiens
57 LARP1 23367
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 ZNF326 284695
Affinity Capture-MS Homo sapiens
59 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 PAIP2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 HNRNPD 3184
Affinity Capture-Western Homo sapiens
63 EIF3G 8666
Affinity Capture-Western Homo sapiens
64 WBSCR16  
Affinity Capture-MS Homo sapiens
65 CLK3  
Affinity Capture-MS Homo sapiens
66 NUBP2 10101
Co-fractionation Homo sapiens
67 PNN 5411
Affinity Capture-MS Homo sapiens
68 CUL3 8452
Affinity Capture-MS Homo sapiens
69 FTH1 2495
Co-fractionation Homo sapiens
70 CELF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 PDCD4 27250
Co-fractionation Homo sapiens
72 NSRP1  
Affinity Capture-MS Homo sapiens
73 DND1  
Affinity Capture-MS Homo sapiens
74 APOBEC3F 200316
Affinity Capture-MS Homo sapiens
75 MYC  
Affinity Capture-MS Homo sapiens
76 RPA3 6119
Proximity Label-MS Homo sapiens
77 ZC3H18  
Affinity Capture-MS Homo sapiens
78 PABPC5 140886
Affinity Capture-MS Homo sapiens
79 SRSF10 10772
Affinity Capture-MS Homo sapiens
80 DDRGK1 65992
Affinity Capture-MS Homo sapiens
81 BMI1  
Affinity Capture-MS Homo sapiens
82 CSDE1 7812
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
83 LMNA 4000
Proximity Label-MS Homo sapiens
84 UPF2 26019
Co-fractionation Homo sapiens
85 FTSJ3 117246
Affinity Capture-MS Homo sapiens
86 LARP4 113251
Affinity Capture-MS Homo sapiens
87 RBMS2 5939
Affinity Capture-MS Homo sapiens
88 PPME1 51400
Affinity Capture-MS Homo sapiens
89 H2AFX 3014
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 YBX1 4904
Affinity Capture-MS Homo sapiens
91 MKRN1 23608
Affinity Capture-MS Homo sapiens
92 RBM45  
Affinity Capture-MS Homo sapiens
93 WDR77 79084
Affinity Capture-MS Homo sapiens
94 SRRM1 10250
Affinity Capture-MS Homo sapiens
95 IREB2 3658
Affinity Capture-MS Homo sapiens
96 SF3B4 10262
Affinity Capture-MS Homo sapiens
97 RPS15 6209
Affinity Capture-MS Homo sapiens
98 NUP35 129401
Proximity Label-MS Homo sapiens
99 SUGP2  
Affinity Capture-MS Homo sapiens
100 MIF 4282
Affinity Capture-MS Homo sapiens
101 WWP2 11060
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
102 NUP50 10762
Co-fractionation Homo sapiens
103 LSM7  
Affinity Capture-MS Homo sapiens
104 SNIP1  
Affinity Capture-MS Homo sapiens
105 MOV10 4343
Affinity Capture-MS Homo sapiens
106 HNRNPL 3191
Affinity Capture-MS Homo sapiens
107 IGF2BP2 10644
Affinity Capture-MS Homo sapiens
108 UPF1 5976
Affinity Capture-MS Homo sapiens
109 PABPC1L 80336
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 HSPA4L 22824
Co-fractionation Homo sapiens
111 PAIP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 KIF1C 10749
Affinity Capture-MS Homo sapiens
113 LUC7L3 51747
Co-fractionation Homo sapiens
114 CASC3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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