Gene description for WDR77
Gene name WD repeat domain 77
Gene symbol WDR77
Other names/aliases HKMT1069
MEP-50
MEP50
Nbla10071
p44
p44/Mep50
Species Homo sapiens
 Database cross references - WDR77
ExoCarta ExoCarta_79084
Entrez Gene 79084
HGNC 29652
MIM 611734
UniProt Q9BQA1  
 WDR77 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for WDR77
Molecular Function
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 IGI
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of cell proliferation GO:0008284 IEA
    negative regulation of epithelial cell proliferation involved in prostate gland development GO:0060770 IEA
    secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development GO:0060528 IEA
    ncRNA metabolic process GO:0034660 TAS
    gene expression GO:0010467 TAS
    chromatin organization GO:0006325 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 IEA
    spliceosomal snRNP assembly GO:0000387 TAS
Subcellular Localization
    methylosome GO:0034709 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IGI
    Golgi apparatus GO:0005794 IDA
    nucleus GO:0005634 IGI
 Experiment description of studies that identified WDR77 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WDR77
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRPB 6628
Reconstituted Complex Homo sapiens
2 PRMT5 10419
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
3 SNRPD1 6632
Reconstituted Complex Homo sapiens
4 SNRPE 6635
Reconstituted Complex Homo sapiens
5 CTDP1  
Invivo Homo sapiens
6 SNRPD3 6634
Reconstituted Complex Homo sapiens
7 CLNS1A 1207
Affinity Capture-MS Homo sapiens
8 SNRPD2 6633
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which WDR77 is involved
PathwayEvidenceSource
RMTs methylate histone arginines TAS Reactome
snRNP Assembly TAS Reactome





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