Gene description for CDK8
Gene name cyclin-dependent kinase 8
Gene symbol CDK8
Other names/aliases K35
Species Homo sapiens
 Database cross references - CDK8
ExoCarta ExoCarta_1024
Vesiclepedia VP_1024
Entrez Gene 1024
HGNC 1779
MIM 603184
UniProt P49336  
 CDK8 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CDK8
Molecular Function
    RNA polymerase II carboxy-terminal domain kinase activity GO:0008353 IEA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    protein kinase activity GO:0004672 TAS
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IEA
Biological Process
    Notch signaling pathway GO:0007219 TAS
    gene expression GO:0010467 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 TAS
    regulation of cell cycle GO:0051726 IEA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    transcription, DNA-templated GO:0006351 TAS
    protein phosphorylation GO:0006468 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    mediator complex GO:0016592 IDA
 Experiment description of studies that identified CDK8 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CDK8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MED19  
Affinity Capture-MS Homo sapiens
2 SMARCB1 6598
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
3 SMARCA2 6595
Co-fractionation Homo sapiens
4 IXL  
Affinity Capture-MS Homo sapiens
5 CRSP6  
Affinity Capture-MS Homo sapiens
6 CCNC  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
7 MED1 5469
Affinity Capture-MS Homo sapiens
8 SMARCC1 6599
Co-fractionation Homo sapiens
9 CRSP2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
10 BRCA1 672
Co-purification Homo sapiens
11 TRG20  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
12 MED6  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
13 THRAP3 9967
Affinity Capture-MS Homo sapiens
14 PCAF  
Affinity Capture-Western Homo sapiens
15 CREBBP  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
16 MED24 9862
Affinity Capture-MS Homo sapiens
17 POLR2A 5430
Invivo Homo sapiens
Invitro Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
18 THRA  
Affinity Capture-Western Homo sapiens
19 MED16 10025
Affinity Capture-MS Homo sapiens
20 MED13 9969
Affinity Capture-MS Homo sapiens
21 MED12  
Affinity Capture-MS Homo sapiens
22 CDKN2B 1030
Invitro Homo sapiens
23 MED23 9439
Affinity Capture-Western Homo sapiens
24 CRSP7  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
25 CEBPB  
Affinity Capture-Western Homo sapiens
26 MED25  
Affinity Capture-MS Homo sapiens
27 MED31  
Affinity Capture-MS Homo sapiens
28 SURB7  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
29 MED9  
Affinity Capture-MS Homo sapiens
30 MED28  
Affinity Capture-MS Homo sapiens
31 SMARCC2 6601
Co-fractionation Homo sapiens
View the network image/svg+xml



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