Gene description for CDK8
Gene name cyclin-dependent kinase 8
Gene symbol CDK8
Other names/aliases K35
Species Homo sapiens
 Database cross references - CDK8
ExoCarta ExoCarta_1024
Vesiclepedia VP_1024
Entrez Gene 1024
HGNC 1779
MIM 603184
UniProt P49336  
 CDK8 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CDK8
Molecular Function
    protein kinase activity GO:0004672 IMP
    protein serine/threonine kinase activity GO:0004674 IBA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein serine kinase activity GO:0106310 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 kinase activity GO:0140833 IEA
    RNA polymerase II CTD heptapeptide repeat S2 kinase activity GO:0140834 IEA
    RNA polymerase II CTD heptapeptide repeat T4 kinase activity GO:0140835 IEA
    RNA polymerase II CTD heptapeptide repeat S5 kinase activity GO:0140836 IEA
    RNA polymerase II CTD heptapeptide repeat S7 kinase activity GO:0140837 IEA
Biological Process
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    regulation of cell cycle GO:0051726 IEA
Subcellular Localization
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    mediator complex GO:0016592 IDA
    mediator complex GO:0016592 IMP
    protein-containing complex GO:0032991 IDA
    CKM complex GO:1990508 IPI
 Experiment description of studies that identified CDK8 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CDK8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GCFC2  
Affinity Capture-MS Homo sapiens
2 SRGAP1 57522
Affinity Capture-MS Homo sapiens
3 CELF1 10658
Affinity Capture-MS Homo sapiens
4 GTF2F1 2962
Affinity Capture-Western Homo sapiens
5 SMC3 9126
Co-purification Homo sapiens
6 UBL4A 8266
Affinity Capture-MS Homo sapiens
7 MED17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
8 MED20 9477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PCF11 51585
Affinity Capture-MS Homo sapiens
10 TRRAP 8295
Co-purification Homo sapiens
11 MED29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 FBXL19  
Affinity Capture-MS Homo sapiens
13 BRCA1 672
Co-purification Homo sapiens
14 MED13L 23389
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 BUD13  
Affinity Capture-MS Homo sapiens
16 MED24 9862
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TAF1  
Affinity Capture-MS Homo sapiens
18 ZNF350  
Affinity Capture-MS Homo sapiens
19 TCEA1 6917
Reconstituted Complex Homo sapiens
20 Med22  
Affinity Capture-MS Mus musculus
21 MED12L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MED13 9969
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 POLR2I 5438
Affinity Capture-MS Homo sapiens
25 FBXW7  
Affinity Capture-MS Homo sapiens
26 BRPF3  
Affinity Capture-MS Homo sapiens
27 MED27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MED22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 POLR2C 5432
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SMAD5 4090
Affinity Capture-Western Homo sapiens
31 USP7 7874
Affinity Capture-Western Homo sapiens
32 SMARCB1 6598
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
33 POLR2H 5437
Affinity Capture-MS Homo sapiens
34 MED4 29079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 WWP1 11059
Affinity Capture-MS Homo sapiens
36 MED1 5469
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 GEMIN5 25929
Affinity Capture-MS Homo sapiens
38 CDK16 5127
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 MED7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
40 NRIP1 8204
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
41 CTDP1 9150
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
42 PARK2  
Affinity Capture-MS Homo sapiens
43 SUPT7L  
Affinity Capture-MS Homo sapiens
44 RBM4 5936
Affinity Capture-MS Homo sapiens
45 BAG6 7917
Affinity Capture-MS Homo sapiens
46 SMARCA4 6597
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
47 PBXIP1 57326
Affinity Capture-MS Homo sapiens
48 SMAD3 4088
Biochemical Activity Homo sapiens
49 MED26  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 HERC2 8924
Affinity Capture-MS Homo sapiens
51 ZNF131  
Affinity Capture-MS Homo sapiens
52 SMAD2 4087
Biochemical Activity Homo sapiens
53 BCL6  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
54 MED25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 MED31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 ACAD11 84129
Affinity Capture-MS Homo sapiens
57 DNAJC12  
Affinity Capture-MS Homo sapiens
58 GET4 51608
Affinity Capture-MS Homo sapiens
59 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
60 H3F3C 440093
Affinity Capture-MS Homo sapiens
61 MED9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 MED15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 SMARCC2 6601
Co-fractionation Homo sapiens
64 POLR2B 5431
Affinity Capture-MS Homo sapiens
65 HNRNPD 3184
Affinity Capture-MS Homo sapiens
66 CCNC  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 HIST2H3C 126961
Biochemical Activity Homo sapiens
68 POLR2E 5434
Affinity Capture-MS Homo sapiens
69 MAML1  
Reconstituted Complex Homo sapiens
70 SREBF1 6720
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
71 CDK19 23097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 POLR2A 5430
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
74 SUZ12  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
75 THRA  
Affinity Capture-Western Homo sapiens
76 SMAD1 4086
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
77 FASN 2194
Positive Genetic Homo sapiens
78 MED10  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 TADA2A  
Affinity Capture-MS Homo sapiens
80 MYC  
Reconstituted Complex Homo sapiens
81 LDLR 3949
Positive Genetic Homo sapiens
82 TOP1 7150
Protein-peptide Homo sapiens
83 EHMT2 10919
Affinity Capture-Western Homo sapiens
84 NIPBL 25836
Co-purification Homo sapiens
85 MED23 9439
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 SMARCA2 6595
Co-fractionation Homo sapiens
87 SMC1A 8243
Co-purification Homo sapiens
88 MED14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 HIF1A 3091
Affinity Capture-Western Homo sapiens
90 MED11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 TBX3 6926
Affinity Capture-MS Homo sapiens
92 DENND4C 55667
Affinity Capture-MS Homo sapiens
93 MED19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 ESR2  
Reconstituted Complex Homo sapiens
95 LZIC 84328
Affinity Capture-MS Homo sapiens
96 RBM45  
Affinity Capture-MS Homo sapiens
97 PARP1 142
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
98 WDR77 79084
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
99 MTBP  
Affinity Capture-MS Homo sapiens
100 TP53 7157
Reconstituted Complex Homo sapiens
101 CDK8 1024
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
102 STK38 11329
Affinity Capture-Western Homo sapiens
103 SMARCC1 6599
Co-fractionation Homo sapiens
104 KAT2B  
Affinity Capture-Western Homo sapiens
105 MED8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 MED6  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 THRAP3 9967
Affinity Capture-MS Homo sapiens
108 CREBBP  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
109 PRMT5 10419
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
110 ESR1  
Reconstituted Complex Homo sapiens
111 MED30  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 MED16 10025
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 MED18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 MED21  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 MED12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 HNRNPF 3185
Affinity Capture-MS Homo sapiens
117 CEBPB  
Affinity Capture-Western Homo sapiens
118 POLR1D 51082
Affinity Capture-MS Homo sapiens
119 AR 367
Proximity Label-MS Homo sapiens
120 MED28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens