Gene description for POLR1D
Gene name polymerase (RNA) I polypeptide D, 16kDa
Gene symbol POLR1D
Other names/aliases AC19
POLR1C
RPA16
RPA9
RPAC2
RPC16
RPO1-3
TCS2
Species Homo sapiens
 Database cross references - POLR1D
ExoCarta ExoCarta_51082
Entrez Gene 51082
HGNC 20422
MIM 613715
UniProt Q9Y2S0  
 POLR1D identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for POLR1D
Molecular Function
    protein binding GO:0005515 IPI
    protein dimerization activity GO:0046983 IEA
    RNA polymerase III activity GO:0001056 IBA
    RNA polymerase I activity GO:0001054 IBA
    DNA binding GO:0003677 IEA
Biological Process
    transcription from RNA polymerase III promoter GO:0006383 TAS
    termination of RNA polymerase III transcription GO:0006386 TAS
    transcription initiation from RNA polymerase I promoter GO:0006361 TAS
    positive regulation of type I interferon production GO:0032481 TAS
    transcription elongation from RNA polymerase I promoter GO:0006362 TAS
    transcription from RNA polymerase I promoter GO:0006360 TAS
    negative regulation of gene expression, epigenetic GO:0045814 TAS
    regulation of gene expression, epigenetic GO:0040029 TAS
    gene expression GO:0010467 TAS
    innate immune response GO:0045087 TAS
    termination of RNA polymerase I transcription GO:0006363 TAS
    transcription elongation from RNA polymerase III promoter GO:0006385 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    DNA-directed RNA polymerase III complex GO:0005666 IBA
    DNA-directed RNA polymerase I complex GO:0005736 IBA
 Experiment description of studies that identified POLR1D in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLR1D
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Far Western Homo sapiens
2 POLR1C 9533
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 MAGEA11  
Two-hybrid Homo sapiens
View the network image/svg+xml



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