Gene description for DDIT3
Gene name DNA-damage-inducible transcript 3
Gene symbol DDIT3
Other names/aliases CEBPZ
CHOP
CHOP-10
CHOP10
GADD153
Species Homo sapiens
 Database cross references - DDIT3
ExoCarta ExoCarta_1649
Entrez Gene 1649
HGNC 2726
MIM 126337
UniProt P35638  
 DDIT3 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for DDIT3
Molecular Function
    protein homodimerization activity GO:0042803 ISS
    transcription regulatory region sequence-specific DNA binding GO:0000976 IDA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    leucine zipper domain binding GO:0043522 IDA
    protein binding GO:0005515 IPI
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 IDA
    transcription corepressor activity GO:0003714 TAS
    transcription regulatory region DNA binding GO:0044212 ISS
    transcription factor binding GO:0008134 IPI
    protein heterodimerization activity GO:0046982 TAS
    DNA binding GO:0003677 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 IGI
    cAMP response element binding protein binding GO:0008140 IPI
Biological Process
    ER overload response GO:0006983 IEA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 IDA
    Wnt signaling pathway GO:0016055 IEA
    cellular protein metabolic process GO:0044267 TAS
    regulation of transcription, DNA-templated GO:0006355 IMP
    mRNA transcription from RNA polymerase II promoter GO:0042789 IDA
    response to starvation GO:0042594 IEA
    response to endoplasmic reticulum stress GO:0034976 IDA
    cell cycle arrest GO:0007050 IDA
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    regulation of transcription involved in anterior/posterior axis specification GO:0044324 ISS
    positive regulation of interleukin-8 production GO:0032757 IMP
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 IC
    release of sequestered calcium ion into cytosol GO:0051209 IEA
    negative regulation of myoblast differentiation GO:0045662 IEA
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    cellular response to DNA damage stimulus GO:0006974 TAS
    negative regulation of determination of dorsal identity GO:2000016 IDA
    positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress GO:1990440 ISS
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    ATF6-mediated unfolded protein response GO:0036500 TAS
    response to unfolded protein GO:0006986 IDA
    negative regulation of protein kinase B signaling GO:0051898 IMP
    negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding GO:1903026 IDA
    negative regulation of canonical Wnt signaling pathway GO:0090090 ISS
    blood vessel maturation GO:0001955 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    positive regulation of neuron apoptotic process GO:0043525 IC
    PERK-mediated unfolded protein response GO:0036499 TAS
    negative regulation of CREB transcription factor activity GO:0032792 IDA
    regulation of DNA-templated transcription in response to stress GO:0043620 TAS
    cell redox homeostasis GO:0045454 IDA
Subcellular Localization
    CHOP-ATF3 complex GO:1990622 IDA
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
    protein-DNA complex GO:0032993 IDA
    AP1 complex GO:0035976 ISS
    CHOP-C/EBP complex GO:0036488 TAS
    late endosome GO:0005770 IEA
    CHOP-ATF4 complex GO:1990617 IPI
 Experiment description of studies that identified DDIT3 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DDIT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SPOP  
Two-hybrid Homo sapiens
2 RPS3A 6189
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 DHRS10  
Two-hybrid Homo sapiens
4 FOSL1  
Affinity Capture-MS Homo sapiens
5 JUND 3727
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 BATF  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 CSNK2A1 1457
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
8 CEBPG  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 MAFG  
Affinity Capture-MS Homo sapiens
10 NFIL3 4783
Affinity Capture-MS Homo sapiens
11 FOSL2  
Affinity Capture-MS Homo sapiens
12 ATF4  
Affinity Capture-MS Homo sapiens
13 JUN 3725
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 DDIT3 1649
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 FOS 2353
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 CREB3  
Affinity Capture-MS Homo sapiens
17 MCMBP 79892
Two-hybrid Homo sapiens
18 CEBPB  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 MAPK14 1432
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
20 HLF 3131
Affinity Capture-MS Homo sapiens
21 PICALM 8301
Affinity Capture-Western Homo sapiens
22 ATF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 TEF  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DDIT3 is involved
PathwayEvidenceSource
ATF4 activates genes TAS Reactome
ATF6-alpha activates chaperone genes TAS Reactome





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