Gene description for JUND
Gene name jun D proto-oncogene
Gene symbol JUND
Other names/aliases AP-1
Species Homo sapiens
 Database cross references - JUND
ExoCarta ExoCarta_3727
Vesiclepedia VP_3727
Entrez Gene 3727
HGNC 6206
MIM 165162
UniProt P17535  
 JUND identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocytes 26054723    
 Gene ontology annotations for JUND
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    transcription coregulator binding GO:0001221 IEA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IEA
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    osteoblast development GO:0002076 IEA
    regulation of transcription by RNA polymerase II GO:0006357 NAS
    gene expression GO:0010467 IEA
    regulation of cell population proliferation GO:0042127 IBA
    positive regulation of osteoblast differentiation GO:0045669 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    response to steroid hormone GO:0048545 IBA
    regulation of cell cycle GO:0051726 IBA
    cellular response to calcium ion GO:0071277 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IBA
    transcription repressor complex GO:0017053 IPI
    transcription factor AP-1 complex GO:0035976 IDA
    RNA polymerase II transcription regulator complex GO:0090575 IPI
 Experiment description of studies that identified JUND in sEVs
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for JUND
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MLLT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RALBP1 10928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 Batf3  
Affinity Capture-MS Mus musculus
4 ARHGAP22 58504
Affinity Capture-MS Homo sapiens
5 ASF1A 25842
Co-localization Homo sapiens
6 SLC25A12 8604
Reconstituted Complex Homo sapiens
7 POT1  
Co-fractionation Homo sapiens
8 RFWD2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 MAPK1 5594
Affinity Capture-Western Homo sapiens
10 PPARG 5468
Reconstituted Complex Homo sapiens
11 FOSL1  
Affinity Capture-MS Homo sapiens
12 NFATC1 4772
Affinity Capture-MS Homo sapiens
13 MEN1 4221
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
14 BRCA1 672
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
15 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
16 BATF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ACTR3 10096
Reconstituted Complex Homo sapiens
18 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
19 CEBPG  
Affinity Capture-MS Homo sapiens
20 YY1 7528
Affinity Capture-Western Homo sapiens
21 CYTH1 9267
Affinity Capture-MS Homo sapiens
22 NUP35 129401
Proximity Label-MS Homo sapiens
23 CREB1  
Affinity Capture-MS Homo sapiens
24 SIRT1  
Affinity Capture-MS Homo sapiens
25 JDP2  
Affinity Capture-Western Homo sapiens
26 FOSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CREB5 9586
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 ATF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 JUN 3725
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
30 CAPZB 832
Affinity Capture-MS Homo sapiens
31 PRKCQ 5588
Affinity Capture-Western Homo sapiens
32 DDIT3 1649
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
33 TPM1 7168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 JUNB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
35 FOS 2353
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 MAPK9 5601
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
37 UBE2O 63893
Affinity Capture-MS Homo sapiens
38 EGR1  
Affinity Capture-MS Homo sapiens
39 SCGN  
Affinity Capture-MS Homo sapiens
40 SMAD3 4088
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
41 PLCH1  
Co-fractionation Homo sapiens
42 COPS5 10987
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
43 MEF2A  
Affinity Capture-MS Homo sapiens
44 CENPQ  
Affinity Capture-MS Homo sapiens
45 HIRA  
Co-localization Homo sapiens
46 BCL6  
Reconstituted Complex Homo sapiens
47 ARIH1 25820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FOSB  
Affinity Capture-MS Homo sapiens
49 UBN1  
Co-localization Homo sapiens
50 LINC00461  
Reconstituted Complex Homo sapiens
51 MAPK8 5599
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
52 NFATC2  
Affinity Capture-MS Homo sapiens
53 ATF3  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
54 CEBPA  
Proximity Label-MS Homo sapiens
55 MDM2  
Reconstituted Complex Homo sapiens
56 HINFP  
Two-hybrid Homo sapiens
57 EP300 2033
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
58 HBZ  
Affinity Capture-Western Homo sapiens
59 TPM3 7170
Affinity Capture-MS Homo sapiens
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