Gene description for ELAVL1
Gene name ELAV like RNA binding protein 1
Gene symbol ELAVL1
Other names/aliases ELAV1
HUR
Hua
MelG
Species Homo sapiens
 Database cross references - ELAVL1
ExoCarta ExoCarta_1994
Entrez Gene 1994
HGNC 3312
MIM 603466
UniProt Q15717  
 ELAVL1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for ELAVL1
Molecular Function
    nucleotide binding GO:0000166 IEA
    RNA binding GO:0003723 IDA
    AU-rich element binding GO:0017091 IDA
    mRNA 3'-UTR binding GO:0003730 ISS
    double-stranded RNA binding GO:0003725 IDA
    poly(A) RNA binding GO:0044822 IDA
    mRNA 3'-UTR AU-rich region binding GO:0035925 IDA
    mRNA binding GO:0003729 TAS
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
Biological Process
    3'-UTR-mediated mRNA stabilization GO:0070935 IMP
    multicellular organismal development GO:0007275 TAS
    regulation of stem cell maintenance GO:2000036 ISS
    positive regulation of translation GO:0045727 IDA
    gene expression GO:0010467 TAS
    mRNA stabilization GO:0048255 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    membrane GO:0016020 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified ELAVL1 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ELAVL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XPO1 7514
Affinity Capture-Western Homo sapiens
2 ELAVL1 1994
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ELAVL1 is involved
PathwayEvidenceSource
HuR stabilizes mRNA TAS Reactome





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