Gene description for PRKCQ
Gene name protein kinase C, theta
Gene symbol PRKCQ
Other names/aliases PRKCT
nPKC-theta
Species Homo sapiens
 Database cross references - PRKCQ
ExoCarta ExoCarta_5588
Entrez Gene 5588
HGNC 9410
MIM 600448
UniProt Q04759  
 PRKCQ identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PRKCQ
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 TAS
    protein kinase C activity GO:0004697 IEA
    protein serine/threonine kinase activity GO:0004674 TAS
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    membrane protein ectodomain proteolysis GO:0006509 ISS
    positive regulation of T-helper 17 type immune response GO:2000318 ISS
    negative regulation of T cell apoptotic process GO:0070233 IMP
    positive regulation of interleukin-17 production GO:0032740 ISS
    blood coagulation GO:0007596 TAS
    phototransduction, visible light GO:0007603 TAS
    axon guidance GO:0007411 TAS
    platelet activation GO:0030168 TAS
    rhodopsin mediated signaling pathway GO:0016056 TAS
    innate immune response GO:0045087 TAS
    inflammatory response GO:0006954 IEA
    positive regulation of T cell proliferation GO:0042102 IEA
    apoptotic process GO:0006915 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    programmed cell death GO:0012501 TAS
    protein phosphorylation GO:0006468 IEA
    T cell receptor signaling pathway GO:0050852 TAS
    positive regulation of T cell activation GO:0050870 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    regulation of platelet aggregation GO:0090330 ISS
    positive regulation of interleukin-4 production GO:0032753 ISS
    positive regulation of T-helper 2 cell activation GO:2000570 ISS
    intracellular signal transduction GO:0035556 NAS
    negative regulation of insulin receptor signaling pathway GO:0046627 IMP
    regulation of rhodopsin mediated signaling pathway GO:0022400 TAS
    protein ubiquitination GO:0016567 TAS
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    regulation of transcription, DNA-templated GO:0006355 ISS
    positive regulation of interleukin-2 biosynthetic process GO:0045086 IEA
    regulation of cell growth GO:0001558 NAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    immunological synapse GO:0001772 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PRKCQ in exosomes
1
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRKCQ
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAG 7532
Affinity Capture-Western Homo sapiens
2 TAL1  
Two-hybrid Homo sapiens
3 GLRX3 10539
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 LCK 3932
Two-hybrid Homo sapiens
5 VAV1 7409
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 IKBKB 3551
Affinity Capture-Western Homo sapiens
7 AKT1 207
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 FYN 2534
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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