Gene description for IKBKG
Gene name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Gene symbol IKBKG
Other names/aliases AMCBX1
FIP-3
FIP3
Fip3p
IKK-gamma
IMD33
IP
IP1
IP2
IPD2
NEMO
ZC2HC9
Species Homo sapiens
 Database cross references - IKBKG
ExoCarta ExoCarta_8517
Vesiclepedia VP_8517
Entrez Gene 8517
HGNC 5961
MIM 300248
UniProt Q9Y6K9  
 IKBKG identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for IKBKG
Molecular Function
    peroxisome proliferator activated receptor binding GO:0042975 IEA
    protein heterodimerization activity GO:0046982 IDA
    linear polyubiquitin binding GO:1990450 IDA
    protein homodimerization activity GO:0042803 IDA
    protein binding GO:0005515 IPI
    K63-linked polyubiquitin binding GO:0070530 IEA
    signal transducer activity GO:0004871 TAS
    ubiquitin protein ligase binding GO:0031625 IPI
    protein domain specific binding GO:0019904 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 TAS
    I-kappaB kinase/NF-kappaB signaling GO:0007249 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    activation of MAPK activity GO:0000187 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    cellular response to DNA damage stimulus GO:0006974 IDA
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    transcription, DNA-templated GO:0006351 IEA
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    positive regulation of type I interferon production GO:0032481 TAS
    immune response GO:0006955 TAS
    negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902236 IEA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    activation of NF-kappaB-inducing kinase activity GO:0007250 IEA
    JNK cascade GO:0007254 TAS
    nucleotide-binding oligomerization domain containing signaling pathway GO:0070423 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    apoptotic process GO:0006915 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    response to virus GO:0009615 TAS
    establishment of vesicle localization GO:0051650 IMP
    innate immune response GO:0045087 TAS
    B cell homeostasis GO:0001782 IEA
    inflammatory response GO:0006954 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    viral process GO:0016032 IEA
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    negative regulation of neuron death GO:1901215 TAS
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
Subcellular Localization
    mitotic spindle GO:0072686 IDA
    ubiquitin ligase complex GO:0000151 IPI
    spindle pole GO:0000922 IDA
    IkappaB kinase complex GO:0008385 TAS
    intracellular GO:0005622 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified IKBKG in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IKBKG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TNFAIP3 7128
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
2 ZFAND5 7763
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 MAP3K14  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 TANK 10010
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
5 TRAF3IP2  
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
6 RNF11 26994
Affinity Capture-MS Homo sapiens
7 HSPA1A  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
8 PPM1B 5495
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 RIPK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
10 CDC37 11140
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 IKBKG 8517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
14 RIPK1 8737
Affinity Capture-Western Homo sapiens
15 TBK1 29110
Affinity Capture-MS Homo sapiens
16 UBB 7314
Affinity Capture-MS Homo sapiens
17 APG16L  
Affinity Capture-MS Homo sapiens
18 RAB11B 9230
Invivo Homo sapiens
Invitro Homo sapiens
19 HSP90AA2P 3324
Affinity Capture-MS Homo sapiens
20 CARD8  
Invivo Homo sapiens
21 NFKB1 4790
Affinity Capture-MS Homo sapiens
22 TAB3 257397
Affinity Capture-MS Homo sapiens
23 COPS3 8533
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
24 NFKB2 4791
Affinity Capture-MS Homo sapiens
25 IKBKB 3551
Invitro Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 CHUK 1147
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
27 TRPC4AP  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
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