Experiment description of studies that identified RAB11FIP1 in exosomes

1 Experiment ID 234 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for RAB11FIP1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PCBP2 5094 Cross-Linking-MS (XL-MS) Homo sapiens 2 ATG16L1 55054 Affinity Capture-MS Homo sapiens 3 RPA2 6118 Co-fractionation Homo sapiens 4 TRIM21 6737 Biochemical Activity Homo sapiens 5 RAB9A 9367 Proximity Label-MS Homo sapiens 6 RPL34 6164 Affinity Capture-MS Homo sapiens 7 SCHIP1   Affinity Capture-MS Homo sapiens 8 RAB35 11021 Proximity Label-MS Homo sapiens 9 RASSF8 11228 Affinity Capture-MS Homo sapiens 10 Klc3   Affinity Capture-MS Mus musculus 11 EBAG9 9166 Proximity Label-MS Homo sapiens 12 FANCD2   Affinity Capture-MS Homo sapiens 13 RAB11FIP5 26056 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 14 LAMTOR1 55004 Proximity Label-MS Homo sapiens 15 MARCKS 4082 Proximity Label-MS Homo sapiens 16 LAMP3   Proximity Label-MS Homo sapiens 17 XPO1 7514 Affinity Capture-MS Homo sapiens 18 YWHAB 7529 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 CTBP2 1488 Affinity Capture-MS Homo sapiens 20 NOTCH2 4853 Affinity Capture-Western Homo sapiens 21 PSMC6 5706 Cross-Linking-MS (XL-MS) Homo sapiens 22 MCM5 4174 Co-fractionation Homo sapiens 23 YWHAE 7531 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 HNRNPK 3190 Cross-Linking-MS (XL-MS) Homo sapiens 25 RAB25 57111 Two-hybrid Homo sapiens 26 PDGFRA 5156 Affinity Capture-MS Homo sapiens 27 PTPRH 5794 Proximity Label-MS Homo sapiens 28 LCK 3932 Proximity Label-MS Homo sapiens 29 YWHAZ 7534 Affinity Capture-MS Homo sapiens 30 ATG9A 79065 Proximity Label-MS Homo sapiens 31 LAMP2 3920 Proximity Label-MS Homo sapiens 32 RAB14 51552 Far Western Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 33 KLC1 3831 Affinity Capture-MS Homo sapiens 34 CXADR 1525 Proximity Label-MS Homo sapiens 35 PIGR 5284 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 36 S100P 6286 Affinity Capture-MS Homo sapiens 37 OCLN 100506658 Proximity Label-MS Homo sapiens 38 RAB11FIP1 80223 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 39 LYN 4067 Proximity Label-MS Homo sapiens 40 DNAJC5 80331 Proximity Label-MS Homo sapiens 41 RAB4A 5867 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens Proximity Label-MS Homo sapiens 42 PPP1CC 5501 Co-fractionation Homo sapiens 43 RAB5A 5868 Proximity Label-MS Homo sapiens 44 PIP4K2A 5305 Affinity Capture-MS Homo sapiens 45 Rab11a 53869 Affinity Capture-MS Mus musculus 46 DDX5 1655 Co-fractionation Homo sapiens 47 MMAB 326625 Affinity Capture-MS Homo sapiens 48 RNF128   Affinity Capture-MS Homo sapiens 49 SLC3A2 6520 Affinity Capture-Western Homo sapiens 50 RELT   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 51 NOLC1 9221 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 UBA6 55236 Co-fractionation Homo sapiens 53 IARS 3376 Cross-Linking-MS (XL-MS) Homo sapiens 54 RAB11B 9230 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 55 RAB11FIP2   Affinity Capture-Western Homo sapiens 56 YWHAH 7533 Affinity Capture-MS Homo sapiens 57 EPHA2 1969 Proximity Label-MS Homo sapiens 58 PTPRJ 5795 Proximity Label-MS Homo sapiens 59 S100B 6285 Affinity Capture-MS Homo sapiens 60 AIMP1 9255 Affinity Capture-Western Homo sapiens 61 KRAS 3845 Proximity Label-MS Homo sapiens 62 RHOB 388 Proximity Label-MS Homo sapiens 63 AR 367 Affinity Capture-MS Homo sapiens 64 RPA1 6117 Co-fractionation Homo sapiens 65 YWHAG 7532 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 66 RNF26   Affinity Capture-MS Homo sapiens 67 ASAP1 50807 Affinity Capture-Western Homo sapiens 68 FAM217B   Affinity Capture-MS Homo sapiens 69 TOM1 10043 Co-fractionation Homo sapiens 70 RPA3 6119 Proximity Label-MS Homo sapiens 71 CAV1 857 Proximity Label-MS Homo sapiens 72 BRD3 8019 Affinity Capture-MS Homo sapiens 73 BAIAP2 10458 Affinity Capture-MS Homo sapiens 74 CBY1   Affinity Capture-MS Homo sapiens 75 FLOT1 10211 Proximity Label-MS Homo sapiens 76 NAA10 8260 Co-fractionation Homo sapiens 77 STX6 10228 Proximity Label-MS Homo sapiens 78 IKBKG 8517 Affinity Capture-MS Homo sapiens 79 RAB11A 8766 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Far Western Homo sapiens View the network

Pathways in which RAB11FIP1 is involved

No pathways found