Gene description for ATG16L1
Gene name autophagy related 16-like 1 (S. cerevisiae)
Gene symbol ATG16L1
Other names/aliases APG16L
ATG16A
ATG16L
IBD10
WDR30
Species Homo sapiens
 Database cross references - ATG16L1
ExoCarta ExoCarta_55054
Vesiclepedia VP_55054
Entrez Gene 55054
HGNC 21498
MIM 610767
UniProt Q676U5  
 ATG16L1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ATG16L1
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin-like protein transferase activity GO:0019787 TAS
    identical protein binding GO:0042802 IPI
    protein-membrane adaptor activity GO:0043495 IBA
    protein-membrane adaptor activity GO:0043495 IMP
    GTPase binding GO:0051020 IPI
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 IMP
    autophagosome assembly GO:0000045 ISO
    autophagosome assembly GO:0000045 NAS
    C-terminal protein lipidation GO:0006501 IDA
    positive regulation of autophagy GO:0010508 IEA
    protein transport GO:0015031 IEA
    macroautophagy GO:0016236 IDA
    macroautophagy GO:0016236 ISO
    macroautophagy GO:0016236 TAS
    microautophagy GO:0016237 IDA
    hippocampus development GO:0021766 IEA
    corpus callosum development GO:0022038 IEA
    protein localization to phagophore assembly site GO:0034497 IEA
    negative stranded viral RNA replication GO:0039689 IEA
    defense response to virus GO:0051607 IEA
    xenophagy GO:0098792 IEA
    axonal transport GO:0098930 IEA
Subcellular Localization
    autophagosome membrane GO:0000421 IBA
    autophagosome membrane GO:0000421 IDA
    autophagosome GO:0005776 ISS
    cytosol GO:0005829 TAS
    axoneme GO:0005930 ISS
    axon GO:0030424 IEA
    phagophore assembly site membrane GO:0034045 IBA
    phagophore assembly site membrane GO:0034045 ISO
    Atg12-Atg5-Atg16 complex GO:0034274 IBA
    Atg12-Atg5-Atg16 complex GO:0034274 IDA
    Atg12-Atg5-Atg16 complex GO:0034274 IPI
    endolysosome membrane GO:0036020 IDA
    sperm midpiece GO:0097225 ISS
    glutamatergic synapse GO:0098978 IEA
    vacuole-isolation membrane contact site GO:0120095 IDA
 Experiment description of studies that identified ATG16L1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ATG16L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CFAP20 29105
Affinity Capture-MS Homo sapiens
2 REV1  
Affinity Capture-MS Homo sapiens
3 EIF3A 8661
Affinity Capture-MS Homo sapiens
4 SLC25A13 10165
Affinity Capture-MS Homo sapiens
5 KRT37 8688
Affinity Capture-MS Homo sapiens
6 CAAP1  
Affinity Capture-MS Homo sapiens
7 ARHGEF2 9181
Affinity Capture-MS Homo sapiens
8 PPP1CB 5500
Affinity Capture-MS Homo sapiens
9 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
10 HDAC2 3066
Affinity Capture-MS Homo sapiens
11 MED17  
Affinity Capture-MS Homo sapiens
12 ZNF346  
Affinity Capture-MS Homo sapiens
13 EIF3C 8663
Affinity Capture-MS Homo sapiens
14 ATG9A 79065
Co-fractionation Homo sapiens
15 PKM 5315
Affinity Capture-MS Homo sapiens
16 SRPK2 6733
Affinity Capture-MS Homo sapiens
17 HACD3 51495
Affinity Capture-MS Homo sapiens
18 RTCA 8634
Affinity Capture-MS Homo sapiens
19 KLC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 EIF2B2 8892
Affinity Capture-MS Homo sapiens
21 UBA1 7317
Affinity Capture-MS Homo sapiens
22 MAP4 4134
Affinity Capture-MS Homo sapiens
23 CLASRP  
Affinity Capture-MS Homo sapiens
24 CALCOCO1 57658
Affinity Capture-MS Homo sapiens
25 UBC 7316
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
26 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
27 FAM32A  
Affinity Capture-MS Homo sapiens
28 MRRF  
Affinity Capture-MS Homo sapiens
29 WDR26 80232
Affinity Capture-MS Homo sapiens
30 GCN1L1 10985
Affinity Capture-MS Homo sapiens
31 VASP 7408
Affinity Capture-MS Homo sapiens
32 SBDS 51119
Affinity Capture-MS Homo sapiens
33 PMF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MAP1LC3B 81631
Affinity Capture-MS Homo sapiens
35 CCNK  
Affinity Capture-MS Homo sapiens
36 CPSF2 53981
Affinity Capture-MS Homo sapiens
37 STRN 6801
Affinity Capture-MS Homo sapiens
38 NOD1 10392
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
39 ABLIM1 3983
Affinity Capture-MS Homo sapiens
40 GPATCH8  
Affinity Capture-MS Homo sapiens
41 HDLBP 3069
Affinity Capture-MS Homo sapiens
42 TRIOBP 11078
Affinity Capture-MS Homo sapiens
43 PPP1R18 170954
Affinity Capture-MS Homo sapiens
44 METAP2 10988
Affinity Capture-MS Homo sapiens
45 ERC1 23085
Affinity Capture-MS Homo sapiens
46 PIP4K2B 8396
Affinity Capture-MS Homo sapiens
47 OIP5  
Affinity Capture-MS Homo sapiens
48 ABI1 10006
Affinity Capture-MS Homo sapiens
49 SMC2 10592
Affinity Capture-MS Homo sapiens
50 RHOA 387
Affinity Capture-MS Homo sapiens
51 DNAJB1 3337
Affinity Capture-MS Homo sapiens
52 EPS15L1 58513
Affinity Capture-MS Homo sapiens
53 EIF3E 3646
Affinity Capture-MS Homo sapiens
54 DYNLL1 8655
Affinity Capture-MS Homo sapiens
55 EXOSC4 54512
Affinity Capture-MS Homo sapiens
56 CENPQ  
Affinity Capture-MS Homo sapiens
57 Inpp5e  
Affinity Capture-Western Mus musculus
58 SEH1L 81929
Affinity Capture-MS Homo sapiens
59 DEF6 50619
Affinity Capture-MS Homo sapiens
60 POLR2C 5432
Affinity Capture-MS Homo sapiens
61 RIPK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 EIF2A 83939
Affinity Capture-MS Homo sapiens
63 PABPN1 8106
Affinity Capture-MS Homo sapiens
64 MSN 4478
Affinity Capture-MS Homo sapiens
65 SLC25A3 5250
Affinity Capture-MS Homo sapiens
66 CLASP2 23122
Affinity Capture-MS Homo sapiens
67 RBMX 27316
Affinity Capture-MS Homo sapiens
68 HEXIM1 10614
Affinity Capture-MS Homo sapiens
69 FARSA 2193
Affinity Capture-MS Homo sapiens
70 KIFC1 3833
Affinity Capture-MS Homo sapiens
71 TCP1 6950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 VAV1 7409
Affinity Capture-MS Homo sapiens
73 MED7  
Affinity Capture-MS Homo sapiens
74 CNOT11 55571
Affinity Capture-MS Homo sapiens
75 RFC5 5985
Affinity Capture-MS Homo sapiens
76 PSMA4 5685
Affinity Capture-MS Homo sapiens
77 MEPCE 56257
Affinity Capture-MS Homo sapiens
78 RABL6 55684
Affinity Capture-MS Homo sapiens
79 RRP9 9136
Affinity Capture-MS Homo sapiens
80 CSTF2 1478
Affinity Capture-MS Homo sapiens
81 TNFAIP8 25816
Affinity Capture-MS Homo sapiens
82 NOD2 64127
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
83 TUFM 7284
Affinity Capture-MS Homo sapiens
84 SUPT16H 11198
Affinity Capture-MS Homo sapiens
85 PABPC1 26986
Affinity Capture-MS Homo sapiens
86 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
87 ACTN4 81
Affinity Capture-MS Homo sapiens
88 OGT 8473
Affinity Capture-MS Homo sapiens
89 CBX5 23468
Affinity Capture-MS Homo sapiens
90 FKBP15 23307
Affinity Capture-MS Homo sapiens
91 BANF1 8815
Affinity Capture-MS Homo sapiens
92 CYC1 1537
Affinity Capture-MS Homo sapiens
93 ENO1 2023
Affinity Capture-MS Homo sapiens
94 SCOC 60592
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 DDX39B 7919
Affinity Capture-MS Homo sapiens
96 ANKRD44 91526
Affinity Capture-MS Homo sapiens
97 PICALM 8301
Affinity Capture-MS Homo sapiens
98 VDAC2 7417
Affinity Capture-MS Homo sapiens
99 SEC61B 10952
Affinity Capture-MS Homo sapiens
100 PDCD7  
Affinity Capture-MS Homo sapiens
101 CACYBP 27101
Affinity Capture-MS Homo sapiens
102 MAP2K7 5609
Affinity Capture-MS Homo sapiens
103 YWHAE 7531
Affinity Capture-MS Homo sapiens
104 ANLN 54443
Affinity Capture-MS Homo sapiens
105 MRPL14 64928
Affinity Capture-MS Homo sapiens
106 RPF2 84154
Affinity Capture-MS Homo sapiens
107 CSTF1 1477
Affinity Capture-MS Homo sapiens
108 CD22 933
Affinity Capture-MS Homo sapiens
109 LMNB1 4001
Affinity Capture-MS Homo sapiens
110 CD79B 974
Affinity Capture-MS Homo sapiens
111 HSPE1 3336
Affinity Capture-MS Homo sapiens
112 TXLNB  
Affinity Capture-MS Homo sapiens
113 NPM3 10360
Affinity Capture-MS Homo sapiens
114 SLC25A1 6576
Affinity Capture-MS Homo sapiens
115 YWHAZ 7534
Affinity Capture-MS Homo sapiens
116 GTPBP1 9567
Affinity Capture-MS Homo sapiens
117 ATRIP  
Affinity Capture-MS Homo sapiens
118 WDR37 22884
Affinity Capture-MS Homo sapiens
119 ZC3H11A  
Affinity Capture-MS Homo sapiens
120 RBBP4 5928
Affinity Capture-MS Homo sapiens
121 SASS6 163786
Affinity Capture-MS Homo sapiens
122 KRT27 342574
Affinity Capture-MS Homo sapiens
123 HEXA 3073
Co-fractionation Homo sapiens
124 YTHDC1  
Affinity Capture-MS Homo sapiens
125 TUBA4A 7277
Affinity Capture-MS Homo sapiens
126 PRMT5 10419
Affinity Capture-MS Homo sapiens
127 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
128 STAT1 6772
Affinity Capture-MS Homo sapiens
129 LTV1  
Affinity Capture-MS Homo sapiens
130 EIF2D 1939
Affinity Capture-MS Homo sapiens
131 SF3B6 51639
Affinity Capture-MS Homo sapiens
132 MED29  
Affinity Capture-MS Homo sapiens
133 HSPA4 3308
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 KDM1A 23028
Affinity Capture-MS Homo sapiens
135 COLEC12 81035
Affinity Capture-MS Homo sapiens
136 EIF2B1 1967
Affinity Capture-MS Homo sapiens
137 INPP5D 3635
Affinity Capture-MS Homo sapiens
138 SPECC1 92521
Affinity Capture-MS Homo sapiens
139 KRT222 125113
Affinity Capture-MS Homo sapiens
140 TPM4 7171
Affinity Capture-MS Homo sapiens
141 DDX55  
Affinity Capture-MS Homo sapiens
142 WDR77 79084
Affinity Capture-MS Homo sapiens
143 NOSIP 51070
Affinity Capture-MS Homo sapiens
144 YBX1 4904
Affinity Capture-MS Homo sapiens
145 GRK5 2869
Affinity Capture-MS Homo sapiens
146 DECR1 1666
Affinity Capture-MS Homo sapiens
147 DOCK2 1794
Affinity Capture-MS Homo sapiens
148 GRB2 2885
Affinity Capture-MS Homo sapiens
149 MRPL22  
Affinity Capture-MS Homo sapiens
150 CHTOP  
Affinity Capture-MS Homo sapiens
151 PLEC 5339
Affinity Capture-MS Homo sapiens
152 ZNF593  
Affinity Capture-MS Homo sapiens
153 THOC3  
Affinity Capture-MS Homo sapiens
154 EIF3I 8668
Affinity Capture-MS Homo sapiens
155 RUVBL2 10856
Affinity Capture-MS Homo sapiens
156 FCGRT 2217
Affinity Capture-MS Homo sapiens
157 LSM14A 26065
Affinity Capture-MS Homo sapiens
158 RRP1 8568
Affinity Capture-MS Homo sapiens
159 CCDC43 124808
Affinity Capture-MS Homo sapiens
160 PNISR  
Affinity Capture-MS Homo sapiens
161 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
162 ITPR3 3710
Affinity Capture-MS Homo sapiens
163 HSP90AA1 3320
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
164 TRMT112 51504
Affinity Capture-MS Homo sapiens
165 TCERG1 10915
Affinity Capture-MS Homo sapiens
166 TRIP11 9321
Affinity Capture-MS Homo sapiens
167 RAB11FIP1 80223
Affinity Capture-MS Homo sapiens
168 SSBP1 6742
Affinity Capture-MS Homo sapiens
169 PSMA1 5682
Affinity Capture-MS Homo sapiens
170 PWP1 11137
Affinity Capture-MS Homo sapiens
171 MED16 10025
Affinity Capture-MS Homo sapiens
172 TBK1 29110
Affinity Capture-MS Homo sapiens
173 PIN1 5300
Affinity Capture-MS Homo sapiens
174 NCL 4691
Affinity Capture-MS Homo sapiens
175 ALYREF 10189
Affinity Capture-MS Homo sapiens
176 EIF4B 1975
Affinity Capture-MS Homo sapiens
177 CLTA 1211
Affinity Capture-MS Homo sapiens
178 KXD1 79036
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
179 ITPR1 3708
Affinity Capture-MS Homo sapiens
180 PPAN 56342
Affinity Capture-MS Homo sapiens
181 CAMK2D 817
Affinity Capture-MS Homo sapiens
182 ADRM1 11047
Affinity Capture-MS Homo sapiens
183 CWC22 57703
Affinity Capture-MS Homo sapiens
184 DPM1 8813
Affinity Capture-MS Homo sapiens
185 PURA 5813
Affinity Capture-MS Homo sapiens
186 BRI3BP 140707
Affinity Capture-MS Homo sapiens
187 POLD2 5425
Affinity Capture-MS Homo sapiens
188 GNAI3 2773
Affinity Capture-MS Homo sapiens
189 PSMA6 5687
Affinity Capture-MS Homo sapiens
190 DENR 8562
Affinity Capture-MS Homo sapiens
191 TRAF3IP3  
Affinity Capture-Western Homo sapiens
192 MCM3 4172
Affinity Capture-MS Homo sapiens
193 SMC3 9126
Affinity Capture-MS Homo sapiens
194 EXOSC7 23016
Affinity Capture-MS Homo sapiens
195 NCBP1 4686
Affinity Capture-MS Homo sapiens
196 KPNA3 3839
Affinity Capture-MS Homo sapiens
197 BCKDK 10295
Affinity Capture-MS Homo sapiens
198 ATG5 9474
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-crystal Structure Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
PCA Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
199 KCTD10 83892
Affinity Capture-MS Homo sapiens
200 RBM15B  
Affinity Capture-MS Homo sapiens
201 TARDBP 23435
Affinity Capture-MS Homo sapiens
202 FMR1 2332
Affinity Capture-MS Homo sapiens
203 RPA2 6118
Affinity Capture-MS Homo sapiens
204 MTPAP 55149
Affinity Capture-MS Homo sapiens
205 ALB 213
Affinity Capture-MS Homo sapiens
206 ALDH2 217
Affinity Capture-MS Homo sapiens
207 THOC7 80145
Affinity Capture-MS Homo sapiens
208 ARL6IP4  
Affinity Capture-MS Homo sapiens
209 TLR2 7097
Affinity Capture-Western Homo sapiens
210 CORO7 79585
Affinity Capture-MS Homo sapiens
211 HSP90AB1 3326
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
212 CCT6B 10693
Affinity Capture-MS Homo sapiens
213 TRAF7  
Affinity Capture-MS Homo sapiens
214 COPA 1314
Affinity Capture-MS Homo sapiens
215 SNRPF 6636
Affinity Capture-MS Homo sapiens
216 CPT1A 1374
Affinity Capture-MS Homo sapiens
217 MEFV  
Affinity Capture-Western Homo sapiens
218 CWF19L2  
Affinity Capture-MS Homo sapiens
219 SAFB 6294
Affinity Capture-MS Homo sapiens
220 WTAP 9589
Affinity Capture-MS Homo sapiens
221 FERMT3 83706
Affinity Capture-MS Homo sapiens
222 RASA2 5922
Affinity Capture-MS Homo sapiens
223 SGPL1 8879
Affinity Capture-MS Homo sapiens
224 G3BP2 9908
Affinity Capture-MS Homo sapiens