Gene description for PDHX
Gene name pyruvate dehydrogenase complex, component X
Gene symbol PDHX
Other names/aliases DLDBP
E3BP
OPDX
PDX1
proX
Species Homo sapiens
 Database cross references - PDHX
ExoCarta ExoCarta_8050
Vesiclepedia VP_8050
Entrez Gene 8050
HGNC 21350
MIM 608769
UniProt O00330  
 PDHX identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PDHX
Molecular Function
    protein binding GO:0005515 IPI
    acyltransferase activity GO:0016746 IEA
    pyruvate dehydrogenase (NAD+) activity GO:0034604 IDA
Biological Process
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 IC
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 IDA
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 ISS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 NAS
    mitochondrial matrix GO:0005759 TAS
    pyruvate dehydrogenase complex GO:0045254 IBA
    pyruvate dehydrogenase complex GO:0045254 IDA
    pyruvate dehydrogenase complex GO:0045254 IPI
    pyruvate dehydrogenase complex GO:0045254 ISS
 Experiment description of studies that identified PDHX in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PDHX
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 MTG2  
Proximity Label-MS Homo sapiens
3 STAT5B 6777
Affinity Capture-MS Homo sapiens
4 FOXD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CS 1431
Co-fractionation Homo sapiens
6 ACTN1 87
Two-hybrid Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 PMPCB 9512
Proximity Label-MS Homo sapiens
9 TACO1  
Proximity Label-MS Homo sapiens
10 TSFM 10102
Proximity Label-MS Homo sapiens
11 LGR4 55366
Affinity Capture-MS Homo sapiens
12 MRRF  
Proximity Label-MS Homo sapiens
13 ILF3 3609
Co-fractionation Homo sapiens
14 SLC25A3 5250
Co-fractionation Homo sapiens
15 PDK3 5165
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MTIF2 4528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 EIF3C 8663
Co-fractionation Homo sapiens
18 MTRF1L  
Proximity Label-MS Homo sapiens
19 STAT5A 6776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 MDH2 4191
Proximity Label-MS Homo sapiens
21 CLPP 8192
Proximity Label-MS Homo sapiens
22 DYNC1I2 1781
Proximity Label-MS Homo sapiens
23 PDK1 5163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 NF2 4771
Affinity Capture-MS Homo sapiens
25 LGALS3 3958
Affinity Capture-MS Homo sapiens
26 DLD 1738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
27 PDHA1 5160
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
28 C6orf203  
Proximity Label-MS Homo sapiens
29 ALDH16A1 126133
Co-fractionation Homo sapiens
30 NR3C1 2908
Affinity Capture-MS Homo sapiens
31 PARS2 25973
Co-fractionation Homo sapiens
32 BRD2  
Affinity Capture-MS Homo sapiens
33 PRMT1 3276
Affinity Capture-MS Homo sapiens
34 PDHB 5162
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
35 HSD17B10 3028
Two-hybrid Homo sapiens
36 CDKN1A  
Affinity Capture-MS Homo sapiens
37 EPRS 2058
Co-fractionation Homo sapiens
38 Hdac6  
Affinity Capture-MS Mus musculus
39 MARC1  
Co-fractionation Homo sapiens
40 MTRF1  
Proximity Label-MS Homo sapiens
41 LARS 51520
Co-fractionation Homo sapiens
42 NXF1 10482
Affinity Capture-RNA Homo sapiens
43 TUFM 7284
Proximity Label-MS Homo sapiens
44 LRPPRC 10128
Co-fractionation Homo sapiens
45 AGTRAP 57085
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 XRCC6 2547
Co-fractionation Homo sapiens
47 MIF 4282
Affinity Capture-MS Homo sapiens
48 HSCB 150274
Proximity Label-MS Homo sapiens
49 PREPL 9581
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CIDEB  
Two-hybrid Homo sapiens
51 GFM1 85476
Proximity Label-MS Homo sapiens
52 OGDH 4967
Co-fractionation Homo sapiens
53 HDAC6 10013
Affinity Capture-MS Homo sapiens
54 HECTD1 25831
Affinity Capture-MS Homo sapiens
55 PMPCA 23203
Proximity Label-MS Homo sapiens
56 ABCC1 4363
Co-fractionation Homo sapiens
57 CCDC109B 55013
Proximity Label-MS Homo sapiens
58 ADSL 158
Co-fractionation Homo sapiens
59 KARS 3735
Co-fractionation Homo sapiens
60 TBRG4 9238
Proximity Label-MS Homo sapiens
61 ACAD9 28976
Proximity Label-MS Homo sapiens
62 SERBP1 26135
Affinity Capture-MS Homo sapiens
63 HINT2 84681
Proximity Label-MS Homo sapiens
64 NEUROD1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
65 HSD17B4 3295
Co-fractionation Homo sapiens
66 RARS 5917
Co-fractionation Homo sapiens
67 C21orf33  
Proximity Label-MS Homo sapiens
68 DHX9 1660
Co-fractionation Homo sapiens
69 TRUB2  
Proximity Label-MS Homo sapiens
70 C17orf80 55028
Proximity Label-MS Homo sapiens
71 METTL17  
Proximity Label-MS Homo sapiens
72 MRPL11 65003
Proximity Label-MS Homo sapiens
73 EWSR1 2130
Two-hybrid Homo sapiens
74 DLAT 1737
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
75 MRPS26 64949
Proximity Label-MS Homo sapiens
76 CAPN2 824
Cross-Linking-MS (XL-MS) Homo sapiens
77 PDK2 5164
Reconstituted Complex Homo sapiens
78 EP300 2033
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
79 CORO1C 23603
Co-fractionation Homo sapiens
80 OGT 8473
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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