Gene description for PARS2
Gene name prolyl-tRNA synthetase 2, mitochondrial (putative)
Gene symbol PARS2
Other names/aliases MT-PRORS
proRS
Species Homo sapiens
 Database cross references - PARS2
ExoCarta ExoCarta_25973
Entrez Gene 25973
HGNC 30563
MIM 612036
UniProt Q7L3T8  
 PARS2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PARS2
Molecular Function
    proline-tRNA ligase activity GO:0004827 IEA
    ATP binding GO:0005524 IEA
Biological Process
    prolyl-tRNA aminoacylation GO:0006433 IEA
    gene expression GO:0010467 TAS
    tRNA aminoacylation for protein translation GO:0006418 TAS
Subcellular Localization
    mitochondrial matrix GO:0005759 IEA
 Experiment description of studies that identified PARS2 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PARS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PARS2 is involved
PathwayEvidenceSource
Mitochondrial tRNA aminoacylation TAS Reactome





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