Gene description for PRKAA2
Gene name protein kinase, AMP-activated, alpha 2 catalytic subunit
Gene symbol PRKAA2
Other names/aliases AMPK
AMPK2
AMPKa2
PRKAA
Species Homo sapiens
 Database cross references - PRKAA2
ExoCarta ExoCarta_5563
Vesiclepedia VP_5563
Entrez Gene 5563
HGNC 9377
MIM 600497
UniProt P54646  
 PRKAA2 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for PRKAA2
Molecular Function
    protein serine/threonine/tyrosine kinase activity GO:0004712 IDA
    metal ion binding GO:0046872 IEA
    histone serine kinase activity GO:0035174 ISS
    chromatin binding GO:0003682 ISS
    AMP-activated protein kinase activity GO:0004679 IDA
    protein serine/threonine kinase activity GO:0004674 ISS
    [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity GO:0047322 IEA
    ATP binding GO:0005524 IEA
    [acetyl-CoA carboxylase] kinase activity GO:0050405 IEA
    protein kinase activity GO:0004672 TAS
    protein binding GO:0005515 IPI
Biological Process
    cell cycle arrest GO:0007050 TAS
    negative regulation of TOR signaling GO:0032007 ISS
    lipid biosynthetic process GO:0008610 ISS
    rhythmic process GO:0048511 IEA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    small molecule metabolic process GO:0044281 TAS
    membrane organization GO:0061024 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
    regulation of energy homeostasis GO:2000505 ISS
    fatty acid homeostasis GO:0055089 ISS
    positive regulation of glycolytic process GO:0045821 ISS
    histone-serine phosphorylation GO:0035404 IBA
    organelle organization GO:0006996 TAS
    intracellular signal transduction GO:0035556 IBA
    regulation of macroautophagy GO:0016241 ISS
    protein phosphorylation GO:0006468 TAS
    gene expression GO:0010467 TAS
    fatty acid biosynthetic process GO:0006633 IEA
    regulation of fatty acid biosynthetic process GO:0042304 TAS
    cholesterol biosynthetic process GO:0006695 IEA
    carnitine shuttle GO:0006853 TAS
    glucose homeostasis GO:0042593 ISS
    negative regulation of apoptotic process GO:0043066 ISS
    cellular response to glucose starvation GO:0042149 ISS
    cellular lipid metabolic process GO:0044255 TAS
    positive regulation of autophagy GO:0010508 ISS
    Wnt signaling pathway GO:0016055 IEA
    cellular response to nutrient levels GO:0031669 ISS
    response to stress GO:0006950 ISS
    mitochondrion organization GO:0007005 TAS
    regulation of circadian rhythm GO:0042752 ISS
    signal transduction GO:0007165 TAS
    energy reserve metabolic process GO:0006112 TAS
    autophagy GO:0006914 IEA
    insulin receptor signaling pathway GO:0008286 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    AMP-activated protein kinase complex GO:0031588 IBA
 Experiment description of studies that identified PRKAA2 in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101|CD63|CD81
EV Enriched markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for PRKAA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LEP  
Invitro Homo sapiens
View the network image/svg+xml



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