Experiment description of studies that identified MLF2 in exosomes

1 Experiment ID 1181 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 32848136     Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 1182 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 32848136     Organism Homo sapiens Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." Journal name Nat Commun Publication year 2020 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

9 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

10 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for MLF2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 STUB1 10273 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 DNAJC7 7266 Affinity Capture-MS Homo sapiens 3 BAG3 9531 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 4 FOXA1   Affinity Capture-MS Homo sapiens 5 ISG15 9636 Affinity Capture-MS Homo sapiens 6 FBXW11   Affinity Capture-MS Homo sapiens 7 NPAS1   Affinity Capture-MS Homo sapiens 8 SPRTN   Affinity Capture-MS Homo sapiens 9 HSPB2   Two-hybrid Homo sapiens 10 PARP1 142 Proximity Label-MS Homo sapiens 11 PDE2A   Affinity Capture-MS Homo sapiens 12 PSMD14 10213 Affinity Capture-MS Homo sapiens 13 ARHGEF4   Affinity Capture-MS Homo sapiens 14 AIFM1 9131 Affinity Capture-MS Homo sapiens 15 HSPA6 3310 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 16 KIF15 56992 Co-fractionation Homo sapiens 17 AMBRA1   Affinity Capture-MS Homo sapiens 18 XPO1 7514 Affinity Capture-MS Homo sapiens 19 BAG2 9532 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 20 INPPL1 3636 Co-fractionation Homo sapiens 21 BAG5 9529 Affinity Capture-MS Homo sapiens 22 POP1 10940 Co-fractionation Homo sapiens 23 DNAJA4 55466 Affinity Capture-MS Homo sapiens 24 PRKAA2 5563 Affinity Capture-MS Homo sapiens 25 FAM96A   Affinity Capture-MS Homo sapiens 26 EBNA-LP   Affinity Capture-MS 27 HYPK 25764 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 28 HSPA1L 3305 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 29 CUL3 8452 Affinity Capture-MS Homo sapiens 30 UBC 7316 Affinity Capture-MS Homo sapiens 31 DNAJB5   Affinity Capture-MS Homo sapiens 32 SRP72 6731 Co-fractionation Homo sapiens 33 RPP30 10556 Co-fractionation Homo sapiens 34 HSP90AA1 3320 Affinity Capture-MS Homo sapiens 35 ZKSCAN1   Affinity Capture-MS Homo sapiens 36 RIPK4   Affinity Capture-MS Homo sapiens 37 GPRASP2   Affinity Capture-MS Homo sapiens 38 FAM96B 51647 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 39 NXF1 10482 Affinity Capture-RNA Homo sapiens 40 DNAJB6 10049 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 41 APEX1 328 Affinity Capture-RNA Homo sapiens 42 DIABLO 56616 Proximity Label-MS Homo sapiens 43 CIAO1 9391 Affinity Capture-MS Homo sapiens 44 MAPK6   Affinity Capture-MS Homo sapiens 45 PTP4A3   Proximity Label-MS Homo sapiens 46 MOV10 4343 Affinity Capture-RNA Homo sapiens 47 BAG1 573 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 48 HSPA2 3306 Affinity Capture-MS Homo sapiens 49 ZNRF4   Affinity Capture-MS Homo sapiens 50 DNAJB4 11080 Affinity Capture-MS Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 51 GCN1L1 10985 Co-fractionation Homo sapiens 52 RGS20 8601 Affinity Capture-MS Homo sapiens 53 USP19 10869 Co-fractionation Homo sapiens 54 METTL3   Affinity Capture-MS Homo sapiens 55 L3MBTL1   Affinity Capture-MS Homo sapiens 56 MMS19 64210 Affinity Capture-MS Homo sapiens 57 HUWE1 10075 Affinity Capture-MS Homo sapiens 58 LRBA 987 Co-fractionation Homo sapiens 59 Bag2   Affinity Capture-MS Mus musculus 60 C9orf72   Affinity Capture-MS Homo sapiens 61 POU5F1   Affinity Capture-MS Homo sapiens 62 AKT1 207 Biochemical Activity Homo sapiens 63 NANOG   Affinity Capture-MS Homo sapiens 64 DNAJB1 3337 Affinity Capture-MS Homo sapiens Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 65 SLC25A12 8604 Proximity Label-MS Homo sapiens 66 ABCD3 5825 Affinity Capture-MS Homo sapiens 67 STIP1 10963 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 68 BAG4   Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens 69 CCDC8   Affinity Capture-MS Homo sapiens 70 AR 367 Affinity Capture-MS Homo sapiens 71 PMVK 10654 Affinity Capture-MS Homo sapiens View the network

Pathways in which MLF2 is involved

No pathways found