|
 Gene ontology annotations for SRP72 |
|
|
| Experiment description of studies that identified SRP72 in exosomes |
| 1 |
| Experiment ID |
412 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX|ACTB
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 2 |
| Experiment ID |
414 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 3 |
| Experiment ID |
426 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 4 |
| Experiment ID |
427 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 5 |
| Experiment ID |
497 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 6 |
| Experiment ID |
498 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 7 |
| Experiment ID |
407 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 8 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 9 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 10 |
| Experiment ID |
420 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 11 |
| Experiment ID |
420 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 12 |
| Experiment ID |
405 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Foreskin fibroblasts |
| Sample name |
BJ |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 13 |
| Experiment ID |
363 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
EV Enriched markers |
|
✔
DCLK1
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M. |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 14 |
| Experiment ID |
364 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
EV Enriched markers |
|
✔
DCLK1
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M. |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 15 |
| Experiment ID |
365 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
EV Enriched markers |
|
✔
DCLK1
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M. |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 16 |
| Experiment ID |
417 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX|ACTB
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Lymphoma cells |
| Sample name |
Raji |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 17 |
| Experiment ID |
411 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Mammary cancer-associated fibroblasts |
| Sample name |
mCAF |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 18 |
| Experiment ID |
488 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Mesenchymal stem cells |
| Sample name |
UCMSC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 19 |
| Experiment ID |
418 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Monocytic leukemia cells |
| Sample name |
THP-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 20 |
| Experiment ID |
418 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Monocytic leukemia cells |
| Sample name |
THP-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 21 |
| Experiment ID |
413 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Normal mammary epithelial cells |
| Sample name |
MCF10A |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 22 |
| Experiment ID |
211 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|EpCAM|TFRC
|
EV Enriched markers |
|
✔
cytochrome c|GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
IGROV1 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 23 |
| Experiment ID |
212 |
| MISEV standards |
|
✔
CEM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|EpCAM|TFRC
|
EV Enriched markers |
|
✔
Cytochrome C|GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
OVCAR-3 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 24 |
| Experiment ID |
406 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
BxPC3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 25 |
| Experiment ID |
415 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 26 |
| Experiment ID |
434 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 27 |
| Experiment ID |
435 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 28 |
| Experiment ID |
408 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX|ACTB
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic duct epithalial cells |
| Sample name |
HPDE |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 29 |
| Experiment ID |
409 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic duct epithalial cells |
| Sample name |
HPNE |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 30 |
| Experiment ID |
416 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pluripotent stem cells |
| Sample name |
PSC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 31 |
| Experiment ID |
410 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
T lymphocytes |
| Sample name |
Jurkat |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 32 |
| Experiment ID |
217 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|CD81|CD9|CD63
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23844026
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of human thymic exosomes. |
| Authors |
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. |
| Journal name |
PLoS One
|
| Publication year |
2013 |
| Sample |
Thymus |
| Sample name |
Normal-Thymus |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| Protein-protein interactions for SRP72 |
|
|
| Pathways in which SRP72 is involved |
|
|
|
