Gene description for EIF4E
Gene name eukaryotic translation initiation factor 4E
Gene symbol EIF4E
Other names/aliases AUTS19
CBP
EIF4E1
EIF4EL1
EIF4F
Species Homo sapiens
 Database cross references - EIF4E
ExoCarta ExoCarta_1977
Entrez Gene 1977
HGNC 3287
MIM 133440
UniProt P06730  
 EIF4E identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for EIF4E
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    protein binding GO:0005515 IPI
    translation initiation factor activity GO:0003743 ISS
    RNA cap binding GO:0000339 TAS
    eukaryotic initiation factor 4G binding GO:0031370 IEA
Biological Process
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    positive regulation of mitotic cell cycle GO:0045931 IMP
    lung development GO:0030324 IEA
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 TAS
    cellular protein metabolic process GO:0044267 TAS
    regulation of translation GO:0006417 IDA
    translation GO:0006412 TAS
    viral process GO:0016032 IEA
    G1/S transition of mitotic cell cycle GO:0000082 IMP
    gene expression GO:0010467 TAS
    translational initiation GO:0006413 TAS
    mRNA export from nucleus GO:0006406 TAS
    insulin receptor signaling pathway GO:0008286 TAS
Subcellular Localization
    eukaryotic translation initiation factor 4F complex GO:0016281 IDA
    cytosol GO:0005829 TAS
    cytoplasmic mRNA processing body GO:0000932 IDA
    mRNA cap binding complex GO:0005845 IDA
    chromatoid body GO:0033391 IEA
    extracellular exosome GO:0070062 IDA
    RISC complex GO:0016442 IDA
    cytoplasmic stress granule GO:0010494 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified EIF4E in exosomes
1
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF4E
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF4A2 1974
Affinity Capture-MS Homo sapiens
2 EIF3B 8662
Affinity Capture-Western Homo sapiens
3 EIF4G3 8672
Affinity Capture-MS Homo sapiens
4 EIF4EBP3  
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
5 EIF4EBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 VCL 7414
Affinity Capture-MS Homo sapiens
7 EIF4A1 1973
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 PML  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
9 EIF4ENIF1 56478
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
10 EIF4E 1977
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 EIF4EBP2 1979
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 EIF4G2  
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
13 MKNK1  
Affinity Capture-Western Homo sapiens
14 EIF4G1 1981
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 EIF4B 1975
Invitro Homo sapiens
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