Gene description for EIF4H
Gene name eukaryotic translation initiation factor 4H
Gene symbol EIF4H
Other names/aliases WBSCR1
WSCR1
eIF-4H
Species Homo sapiens
 Database cross references - EIF4H
ExoCarta ExoCarta_7458
Entrez Gene 7458
HGNC 12741
MIM 603431
UniProt Q15056  
 EIF4H identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Urine 15326289    
 Gene ontology annotations for EIF4H
Molecular Function
    RNA binding GO:0003723 TAS
    protein binding GO:0005515 IPI
    translation factor activity, RNA binding GO:0008135 TAS
    nucleotide binding GO:0000166 IEA
    translation initiation factor activity GO:0003743 IEA
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    regulation of translational initiation GO:0006446 TAS
    gene expression GO:0010467 TAS
    translation GO:0006412 TAS
    viral process GO:0016032 IEA
    sexual reproduction GO:0019953 IEA
    developmental growth GO:0048589 IEA
    translational initiation GO:0006413 TAS
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IEA
    membrane GO:0016020 IDA
    eukaryotic translation initiation factor 4F complex GO:0016281 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified EIF4H in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 274
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
3
Experiment ID 13
ISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
CD9|AQP2|AQP1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for EIF4H
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Bles03  
Two-hybrid Homo sapiens
View the network image/svg+xml



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