Experiment description of studies that identified TGFB1 in exosomes |
1 |
Experiment ID |
489 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
EV Enriched markers |
✔
Canx
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 6 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
2 |
Experiment ID |
490 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
EV Enriched markers |
✔
Canx
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
3 |
Experiment ID |
491 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
EV Enriched markers |
✔
Canx
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 8 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
4 |
Experiment ID |
492 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
EV Enriched markers |
✔
Canx
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 9 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
5 |
Experiment ID |
126 |
MISEV standards |
✘
|
EV Biophysical techniques |
✔
GAPDH
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
PubMed ID |
Unpublished / Not applicable
|
Organism |
Homo sapiens |
Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
Authors |
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim |
Journal name |
Mesenchymal Stem Cell Therapy
|
Publication year |
2011 |
Sample |
Mesenchymal stem cells |
Sample name |
huES9.E1 |
Isolation/purification methods |
HPLC |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Antobody array Mass spectrometry |
|
|
6 |
Experiment ID |
231 |
MISEV standards |
✘
|
EV Biophysical techniques |
✔
Alix|CD63|CD9
|
EV Enriched markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25332113
|
Organism |
Homo sapiens |
Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
Journal name |
Transfusion
|
Publication year |
2015 |
Sample |
Platelets |
Sample name |
PL-Exs - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient |
Flotation density |
1.12-1.15 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
7 |
Experiment ID |
232 |
MISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25332113
|
Organism |
Homo sapiens |
Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
Journal name |
Transfusion
|
Publication year |
2015 |
Sample |
Platelets |
Sample name |
PL-Exs - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient |
Flotation density |
1.12-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
8 |
Experiment ID |
233 |
MISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25332113
|
Organism |
Homo sapiens |
Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
Journal name |
Transfusion
|
Publication year |
2015 |
Sample |
Platelets |
Sample name |
PL-Exs - Rep 3 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient |
Flotation density |
1.12-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
9 |
Experiment ID |
250 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
GAPDH|TSG101|CD63|CD81
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25857301
|
Organism |
Homo sapiens |
Experiment description |
Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles. |
Authors |
Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D |
Journal name |
Oncotarget
|
Publication year |
2015 |
Sample |
Prostate cancer cells |
Sample name |
DU145 - expressing DIAPH3 hRNA |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation OptiPrep density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
10 |
Experiment ID |
250 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
GAPDH|TSG101|CD63|CD81
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25857301
|
Organism |
Homo sapiens |
Experiment description |
Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles. |
Authors |
Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D |
Journal name |
Oncotarget
|
Publication year |
2015 |
Sample |
Prostate cancer cells |
Sample name |
DU145 - expressing DIAPH3 hRNA |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation OptiPrep density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
11 |
Experiment ID |
29 |
MISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
19109410
|
Organism |
Homo sapiens |
Experiment description |
Proteomic and immunologic analyses of brain tumor exosomes. |
Authors |
Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD |
Journal name |
FASEB
|
Publication year |
2008 |
Sample |
Serum |
Sample name |
Serum - Brain tumor |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|