Gene description for RPS4X
Gene name ribosomal protein S4, X-linked
Gene symbol RPS4X
Other names/aliases CCG2
DXS306
RPS4
S4
SCAR
SCR10
Species Homo sapiens
 Database cross references - RPS4X
ExoCarta ExoCarta_6191
Vesiclepedia VP_6191
Entrez Gene 6191
HGNC 10424
MIM 312760
UniProt P62701  
 RPS4X identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 19837982    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for RPS4X
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 HDA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 IMP
    protein binding GO:0005515 IPI
    rRNA binding GO:0019843 IEA
Biological Process
    cytoplasmic translation GO:0002181 IC
    translation GO:0006412 IBA
    translation GO:0006412 IC
    translation GO:0006412 IMP
    positive regulation of cell population proliferation GO:0008284 IMP
    ribosomal small subunit biogenesis GO:0042274 IDA
    positive regulation of translation GO:0045727 IMP
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IEA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 HDA
    ribosome GO:0005840 IDA
    focal adhesion GO:0005925 HDA
    small ribosomal subunit GO:0015935 IDA
    membrane GO:0016020 HDA
    cytosolic ribosome GO:0022626 IDA
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 NAS
    small-subunit processome GO:0032040 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
    synapse GO:0045202 IEA
    extracellular exosome GO:0070062 HDA
    ribonucleoprotein complex GO:1990904 IDA
 Experiment description of studies that identified RPS4X in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
5
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
11
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
15
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
16
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
17
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
18
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
19
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
20
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
21
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
22
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
27
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
29
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
30
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
31
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
34
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
35
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
36
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
37
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
38
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
39
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
40
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
41
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
43
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
44
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
45
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
46
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
47
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
48
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
49
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
50
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
51
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
52
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
53
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
54
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
55
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
56
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
57
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
58
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPS4X
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCL3  
Affinity Capture-MS Homo sapiens
2 RPSAP58 388524
Affinity Capture-MS Homo sapiens
3 UBL4A 8266
Affinity Capture-MS Homo sapiens
4 EIF3C 8663
Co-fractionation Homo sapiens
5 GGPS1  
Affinity Capture-MS Homo sapiens
6 RIN3  
Affinity Capture-MS Homo sapiens
7 SKI 6497
Affinity Capture-MS Homo sapiens
8 EBNA-LP  
Affinity Capture-MS
9 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
10 KIF20A 10112
Affinity Capture-MS Homo sapiens
11 SLC25A1 6576
Co-fractionation Homo sapiens
12 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
13 RPL12 6136
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 RB1CC1 9821
Affinity Capture-MS Homo sapiens
16 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
17 KIF23 9493
Affinity Capture-MS Homo sapiens
18 TRDN 10345
Affinity Capture-MS Homo sapiens
19 CALD1 800
Affinity Capture-MS Homo sapiens
20 FBL 2091
Co-fractionation Homo sapiens
21 RPS9 6203
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
22 GSPT1 2935
Affinity Capture-MS Homo sapiens
23 FAM207A  
Co-crystal Structure Homo sapiens
24 EXOSC4 54512
Affinity Capture-MS Homo sapiens
25 LRRC46  
Affinity Capture-MS Homo sapiens
26 MECP2 4204
Affinity Capture-MS Homo sapiens
27 ARIH2 10425
Affinity Capture-MS Homo sapiens
28 RPS11 6205
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
29 EEF1A1 1915
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
30 KIF14 9928
Affinity Capture-MS Homo sapiens
31 NOTCH2 4853
Affinity Capture-MS Homo sapiens
32 EMC9  
Affinity Capture-MS Homo sapiens
33 CHMP4C 92421
Affinity Capture-MS Homo sapiens
34 AURKA 6790
Affinity Capture-MS Homo sapiens
35 MEPCE 56257
Affinity Capture-MS Homo sapiens
36 RPL18A 6142
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
37 PABPC1 26986
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
38 Srp72  
Affinity Capture-MS Mus musculus
39 YAP1 10413
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TRIM21 6737
Affinity Capture-MS Homo sapiens
41 SLX4  
Affinity Capture-MS Homo sapiens
42 RPL31 6160
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
43 NOS2  
Affinity Capture-MS Homo sapiens
44 CUL2 8453
Affinity Capture-MS Homo sapiens
45 DDX39B 7919
Affinity Capture-MS Homo sapiens
46 SEC61B 10952
Affinity Capture-MS Homo sapiens
47 TRIM63  
Two-hybrid Homo sapiens
48 SLC25A5 292
Co-fractionation Homo sapiens
49 ANLN 54443
Affinity Capture-MS Homo sapiens
50 EIF3G 8666
Affinity Capture-MS Homo sapiens
51 HIST1H2AD 3013
Co-fractionation Homo sapiens
52 TMPO 7112
Affinity Capture-MS Homo sapiens
53 RBM39 9584
Affinity Capture-MS Homo sapiens
54 SIRT7  
Affinity Capture-MS Homo sapiens
55 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 WIBG 84305
Co-fractionation Homo sapiens
57 FN1 2335
Affinity Capture-MS Homo sapiens
58 PCBP1 5093
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
59 RPL17 6139
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
60 RPA3 6119
Proximity Label-MS Homo sapiens
61 RPL35A 6165
Co-fractionation Homo sapiens
62 DCC  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
63 EIF3CL 728689
Co-fractionation Homo sapiens
64 Rpl35 66489
Affinity Capture-MS Mus musculus
65 PEX3 8504
Proximity Label-MS Homo sapiens
66 GNL3 26354
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
67 RPS16 6217
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
68 ARPC3 10094
Two-hybrid Homo sapiens
69 RPS27A 6233
Co-fractionation Homo sapiens
70 PDE4B  
Protein-RNA Homo sapiens
71 WDR77 79084
Affinity Capture-MS Homo sapiens
72 RRS1 23212
Co-fractionation Homo sapiens
73 USP1 7398
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
74 RUVBL2 10856
Affinity Capture-MS Homo sapiens
75 ZEB1  
Affinity Capture-MS Homo sapiens
76 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
77 G3BP2 9908
Affinity Capture-MS Homo sapiens
78 KRR1 11103
Co-fractionation Homo sapiens
79 NEIL1  
Affinity Capture-MS Homo sapiens
80 RPS24 6229
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
81 NCL 4691
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 EIF4B 1975
Affinity Capture-MS Homo sapiens
83 ATG4B 23192
Affinity Capture-MS Homo sapiens
84 CUL7 9820
Affinity Capture-MS Homo sapiens
85 CAMK2D 817
Two-hybrid Homo sapiens
86 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
87 SPRTN  
Affinity Capture-MS Homo sapiens
88 ATG5 9474
Proximity Label-MS Homo sapiens
89 EIF2S3 1968
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
90 RPS17 6218
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
91 CTBP2 1488
Two-hybrid Homo sapiens
92 TARDBP 23435
Affinity Capture-MS Homo sapiens
93 BTF3 689
Affinity Capture-MS Homo sapiens
94 NOP56 10528
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
95 RPL14 9045
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
96 EIF4A1 1973
Affinity Capture-MS Homo sapiens
97 CAND1 55832
Affinity Capture-MS Homo sapiens
98 RPS3 6188
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
99 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
100 RPS18 6222
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
101 METAP2 10988
Affinity Capture-MS Homo sapiens
102 B3GNT2 10678
Affinity Capture-MS Homo sapiens
103 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
104 NOLC1 9221
Co-fractionation Homo sapiens
105 COPS6 10980
Affinity Capture-MS Homo sapiens
106 PRC1 9055
Affinity Capture-MS Homo sapiens
107 NPM1 4869
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
108 OBSL1 23363
Affinity Capture-MS Homo sapiens
109 U2AF1 7307
Co-fractionation Homo sapiens
110 HIST1H2AA 221613
Co-fractionation Homo sapiens
111 DDX6 1656
Affinity Capture-MS Homo sapiens
112 CUL1 8454
Affinity Capture-MS Homo sapiens
113 RBM42  
Affinity Capture-MS Homo sapiens
114 RPL10L 140801
Co-fractionation Homo sapiens
115 PFN1 5216
Affinity Capture-MS Homo sapiens
116 ACTA2 59
Co-fractionation Homo sapiens
117 EIF6 3692
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
118 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
119 CDKN1A  
Affinity Capture-MS Homo sapiens
120 RPL4 6124
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
121 DRG1 4733
Affinity Capture-MS Homo sapiens
122 VRK1 7443
Affinity Capture-MS Homo sapiens
123 TUBB2B 347733
Co-fractionation Homo sapiens
124 RLIM 51132
Affinity Capture-MS Homo sapiens
125 LUC7L2 51631
Co-fractionation Homo sapiens
126 Rrbp1  
Affinity Capture-MS Mus musculus
127 RPLP0 6175
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
128 RPLP0P6 220717
Co-fractionation Homo sapiens
129 RAD21 5885
Affinity Capture-Western Homo sapiens
130 RPL29 6159
Co-fractionation Homo sapiens
131 NEDD1 121441
Affinity Capture-MS Homo sapiens
132 FGFBP1 9982
Affinity Capture-MS Homo sapiens
133 RPL32 6161
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
134 COPS7A 50813
Cross-Linking-MS (XL-MS) Homo sapiens
135 RPN2 6185
Proximity Label-MS Homo sapiens
136 RPS2 6187
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
137 XPO1 7514
Affinity Capture-MS Homo sapiens
138 USP15 9958
Affinity Capture-MS Homo sapiens
139 RC3H2  
Affinity Capture-MS Homo sapiens
140 EGFR 1956
Negative Genetic Homo sapiens
141 RPS27 6232
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
142 CDK2 1017
Affinity Capture-MS Homo sapiens
143 CEP250 11190
Affinity Capture-MS Homo sapiens
144 MYC  
Affinity Capture-MS Homo sapiens
145 HMGB3 3149
Cross-Linking-MS (XL-MS) Homo sapiens
146 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
147 RPL23A 6147
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
148 DDRGK1 65992
Affinity Capture-MS Homo sapiens
149 RPL26 6154
Co-fractionation Homo sapiens
150 HUWE1 10075
Affinity Capture-MS Homo sapiens
151 RPL19 6143
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
152 RPL13 6137
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
153 SPOP  
Affinity Capture-MS Homo sapiens
154 ILF3 3609
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
155 TP53 7157
Affinity Capture-MS Homo sapiens
156 FOLR1 2348
Affinity Capture-MS Homo sapiens
157 SRP19 6728
Affinity Capture-MS Homo sapiens
158 RPL23 9349
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
159 RPS10P5 93144
Co-fractionation Homo sapiens
160 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
161 RPS5 6193
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
162 POU2F1 5451
Affinity Capture-MS Homo sapiens
163 ESR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
164 BAG5 9529
Affinity Capture-MS Homo sapiens
165 RPL7A 6130
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
166 SSSCA1 10534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
167 KRAS 3845
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
168 PTRF 284119
Co-fractionation Homo sapiens
169 BAG1 573
Affinity Capture-MS Homo sapiens
170 UBE2H 7328
Affinity Capture-MS Homo sapiens
171 SENP3 26168
Affinity Capture-MS Homo sapiens
172 RIOK1 83732
Co-crystal Structure Homo sapiens
173 NOP58 51602
Co-fractionation Homo sapiens
174 RPS19 6223
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
175 BYSL 705
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
176 SSB 6741
Affinity Capture-MS Homo sapiens
177 RPS6KB2  
Affinity Capture-MS Homo sapiens
178 FAU 2197
Co-fractionation Homo sapiens
179 ATG13 9776
Affinity Capture-MS Homo sapiens
180 CAPZB 832
Affinity Capture-MS Homo sapiens
181 RPL10A 4736
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
182 MCM2 4171
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
183 EIF3B 8662
Affinity Capture-MS Homo sapiens
184 SRP14 6727
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
185 ITGA4 3676
Affinity Capture-MS Homo sapiens
186 LOC101929876 101929876
Co-fractionation Homo sapiens
187 DDX18 8886
Co-fractionation Homo sapiens
188 TFCP2 7024
Affinity Capture-MS Homo sapiens
189 CYLD  
Affinity Capture-MS Homo sapiens
190 RBM8A 9939
Affinity Capture-MS Homo sapiens
191 HSPA8 3312
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
192 NUP155 9631
Proximity Label-MS Homo sapiens
193 BRIX1 55299
Co-fractionation Homo sapiens
194 RPS4Y2 140032
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
195 NF2 4771
Affinity Capture-MS Homo sapiens
196 RPL27 6155
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
197 S100A2 6273
Affinity Capture-MS Homo sapiens
198 RPL9 6133
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
199 CUL4B 8450
Affinity Capture-MS Homo sapiens
200 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
201 IGF1R 3480
Affinity Capture-MS Homo sapiens
202 RPS10 6204
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
203 XRN1 54464
Co-fractionation Homo sapiens
204 NOP2 4839
Co-fractionation Homo sapiens
205 RPL5 6125
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
206 CHMP4B 128866
Affinity Capture-MS Homo sapiens
207 NTRK1 4914
Affinity Capture-MS Homo sapiens
208 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
209 RPL36 25873
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
210 RPA4  
Proximity Label-MS Homo sapiens
211 RPLP1 6176
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
212 ENY2 56943
Affinity Capture-MS Homo sapiens
213 HDAC5 10014
Affinity Capture-MS Homo sapiens
214 HMGB2 3148
Affinity Capture-MS Homo sapiens
215 RPL11 6135
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
216 LARP1 23367
Co-fractionation Homo sapiens
217 OGT 8473
Reconstituted Complex Homo sapiens
218 EFNA4  
Affinity Capture-MS Homo sapiens
219 METTL14  
Affinity Capture-MS Homo sapiens
220 ECT2 1894
Affinity Capture-MS Homo sapiens
221 EEF2 1938
Co-fractionation Homo sapiens
222 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
223 RPL21 6144
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
224 FBXO6 26270
Affinity Capture-MS Homo sapiens
225 RIOK3 8780
Affinity Capture-MS Homo sapiens
226 NCAPH 23397
Affinity Capture-MS Homo sapiens
227 PSPC1 55269
Affinity Capture-MS Homo sapiens
228 RPS13 6207
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
229 RPS14 6208
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
230 NHP2L1 4809
Co-fractionation Homo sapiens
231 RPL22 6146
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
232 HP1BP3 50809
Cross-Linking-MS (XL-MS) Homo sapiens
233 RPL18 6141
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
234 Eif3e 16341
Affinity Capture-MS Mus musculus
235 TSR1 55720
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
236 AHSA1 10598
Affinity Capture-MS Homo sapiens
237 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
238 RPS25 6230
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
239 FBXL6  
Affinity Capture-MS Homo sapiens
240 RPL6 6128
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
241 RPS21 6227
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
242 ZZEF1 23140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
243 EFNA1  
Affinity Capture-MS Homo sapiens
244 HNRNPM 4670
Co-fractionation Homo sapiens
245 G3BP1 10146
Affinity Capture-MS Homo sapiens
246 PDZD8 118987
Affinity Capture-MS Homo sapiens
247 UFL1 23376
Affinity Capture-MS Homo sapiens
248 RPL38 6169
Co-fractionation Homo sapiens
249 API5 8539
Affinity Capture-MS Homo sapiens
250 HNRNPH3 3189
Two-hybrid Homo sapiens
251 RTCB 51493
Affinity Capture-MS Homo sapiens
252 RPS7 6201
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
253 RPL8 6132
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
254 RPS27L 51065
Co-fractionation Homo sapiens
255 AR 367
Affinity Capture-MS Homo sapiens
256 RC3H1 149041
Affinity Capture-MS Homo sapiens
257 ATXN3 4287
Affinity Capture-MS Homo sapiens
258 SSRP1 6749
Affinity Capture-MS Homo sapiens
259 EIF5 1983
Affinity Capture-MS Homo sapiens
260 UBA52 7311
Co-fractionation Homo sapiens
261 EIF3L 51386
Co-fractionation Homo sapiens
262 RPSA 3921
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
263 RPS12 6206
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
264 RIT1 6016
Negative Genetic Homo sapiens
265 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
266 DDX24 57062
Co-fractionation Homo sapiens
267 COIL  
Proximity Label-MS Homo sapiens
268 RPS3A 6189
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
269 RPL30 6156
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
270 PPIE 10450
Affinity Capture-MS Homo sapiens
271 RPL13A 23521
Co-fractionation Homo sapiens
272 RPL37A 6168
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
273 RPL10 6134
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
274 RPL15 6138
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
275 TRIM55  
Two-hybrid Homo sapiens
276 ZBTB1  
Affinity Capture-MS Homo sapiens
277 BRWD1  
Cross-Linking-MS (XL-MS) Homo sapiens
278 ILF2 3608
Co-fractionation Homo sapiens
279 USP36  
Affinity Capture-MS Homo sapiens
280 KIAA1429 25962
Affinity Capture-MS Homo sapiens
281 TOP2A 7153
Affinity Capture-MS Homo sapiens
282 RPS20 6224
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
283 TGFB1 7040
Affinity Capture-MS Homo sapiens
284 COPS5 10987
Affinity Capture-MS Homo sapiens
285 FBXW7  
Affinity Capture-MS Homo sapiens
286 RPL24 6152
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
287 PSMD9 5715
Affinity Capture-MS Homo sapiens
288 CALM3 808
Affinity Capture-MS Homo sapiens
289 ABCE1 6059
Affinity Capture-MS Homo sapiens
290 RAD51  
Affinity Capture-MS Homo sapiens
291 EED  
Affinity Capture-MS Homo sapiens
292 SRP68 6730
Affinity Capture-MS Homo sapiens
293 CEP57  
Affinity Capture-MS Homo sapiens
294 MYCN  
Affinity Capture-MS Homo sapiens
295 CTCF  
Affinity Capture-MS Homo sapiens
296 RPS26 6231
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
297 TPT1 7178
Affinity Capture-MS Homo sapiens
298 RPS23 6228
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
299 SRP9 6726
Affinity Capture-MS Homo sapiens
300 MAGEA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
301 RPS28 6234
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
302 STAU1 6780
Affinity Capture-MS Homo sapiens
303 SRSF6 6431
Affinity Capture-MS Homo sapiens
304 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
305 IFI16 3428
Affinity Capture-MS Homo sapiens
306 RPL27A 6157
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
307 CEP76  
Affinity Capture-MS Homo sapiens
308 RPS15A 6210
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
309 VCAM1 7412
Affinity Capture-MS Homo sapiens
310 GAN 8139
Affinity Capture-MS Homo sapiens
311 RPLP2 6181
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
312 FANCD2  
Affinity Capture-MS Homo sapiens
313 CDK9 1025
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
314 PANX1 24145
Proximity Label-MS Homo sapiens
315 DNAJC2 27000
Affinity Capture-MS Homo sapiens
316 RPL35 11224
Co-fractionation Homo sapiens
317 CTNNB1 1499
Affinity Capture-MS Homo sapiens
318 KCTD12 115207
Affinity Capture-MS Homo sapiens
319 RPS6 6194
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
320 RPL3 6122
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
321 NAP1L1 4673
Co-fractionation Homo sapiens
322 RPL7 6129
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
323 AURKB 9212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
324 DKC1 1736
Co-fractionation Homo sapiens
325 HIST1H2BA 255626
Cross-Linking-MS (XL-MS) Homo sapiens
326 RPS4Y1 6192
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
327 RPS29 6235
Co-fractionation Homo sapiens
328 NOL6 65083
Co-fractionation Homo sapiens
329 GNB2L1 10399
Affinity Capture-MS Homo sapiens
330 CCDC8  
Affinity Capture-MS Homo sapiens
331 EIF3D 8664
Co-fractionation Homo sapiens
332 ARRB2 409
Affinity Capture-MS Homo sapiens
333 ESR2  
Affinity Capture-MS Homo sapiens
334 GAR1 54433
Co-fractionation Homo sapiens
335 TUBG1 7283
Affinity Capture-MS Homo sapiens
336 HECTD3 79654
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
337 CUL5 8065
Affinity Capture-MS Homo sapiens
338 SMURF1 57154
Affinity Capture-MS Homo sapiens
339 RPS15 6209
Reconstituted Complex Homo sapiens
340 PRMT1 3276
Affinity Capture-MS Homo sapiens
341 SRP72 6731
Affinity Capture-MS Homo sapiens
342 RPL39 6170
Co-fractionation Homo sapiens
343 TRIP4 9325
Affinity Capture-MS Homo sapiens
344 TRIM31  
Affinity Capture-MS Homo sapiens
345 RPS8 6202
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
346 XRCC6 2547
Proximity Label-MS Homo sapiens
347 HNRNPU 3192
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
348 C9orf72  
Affinity Capture-MS Homo sapiens
349 TRIM29 23650
Affinity Capture-MS Homo sapiens
350 EXOSC1 51013
Affinity Capture-MS Homo sapiens
351 NEDD8 4738
Affinity Capture-MS Homo sapiens
352 CDC5L 988
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which RPS4X is involved
PathwayEvidenceSource
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S TAS Reactome
Axon guidance IEA Reactome
Cap-dependent Translation Initiation TAS Reactome
Cellular response to starvation IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Eukaryotic Translation Elongation IEA Reactome
Eukaryotic Translation Initiation TAS Reactome
Eukaryotic Translation Termination IEA Reactome
Formation of a pool of free 40S subunits TAS Reactome
Formation of the ternary complex, and subsequently, the 43S complex TAS Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit TAS Reactome
Infectious disease TAS Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
L13a-mediated translational silencing of Ceruloplasmin expression TAS Reactome
Major pathway of rRNA processing in the nucleolus and cytosol TAS Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Nervous system development IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) TAS Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) TAS Reactome
Nonsense-Mediated Decay (NMD) TAS Reactome
Peptide chain elongation IEA Reactome
Regulation of expression of SLITs and ROBOs IEA Reactome
Response of EIF2AK4 (GCN2) to amino acid deficiency IEA Reactome
Ribosomal scanning and start codon recognition TAS Reactome
rRNA processing TAS Reactome
rRNA processing in the nucleus and cytosol TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-1 modulates host translation machinery TAS Reactome
SARS-CoV-1-host interactions TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2 modulates host translation machinery TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
Selenoamino acid metabolism IEA Reactome
Selenocysteine synthesis IEA Reactome
Signaling by ROBO receptors IEA Reactome
SRP-dependent cotranslational protein targeting to membrane IEA Reactome
SRP-dependent cotranslational protein targeting to membrane TAS Reactome
Translation TAS Reactome
Translation IEA Reactome
Translation initiation complex formation TAS Reactome
Viral Infection Pathways TAS Reactome
Viral mRNA Translation TAS Reactome





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