Gene description for SLC25A1
Gene name solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
Gene symbol SLC25A1
Other names/aliases CTP
D2L2AD
SEA
SLC20A3
Species Homo sapiens
 Database cross references - SLC25A1
ExoCarta ExoCarta_6576
Vesiclepedia VP_6576
Entrez Gene 6576
HGNC 10979
MIM 190315
UniProt P53007  
 SLC25A1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Urine 19056867    
 Gene ontology annotations for SLC25A1
Molecular Function
    citrate transmembrane transporter activity GO:0015137 TAS
    tricarboxylic acid transmembrane transporter activity GO:0015142 IDA
    tricarboxylic acid transmembrane transporter activity GO:0015142 TAS
    antiporter activity GO:0015297 IEA
    citrate secondary active transmembrane transporter activity GO:0071913 IBA
    citrate secondary active transmembrane transporter activity GO:0071913 IDA
Biological Process
    monoatomic ion transport GO:0006811 TAS
    mitochondrial citrate transmembrane transport GO:0006843 IBA
    mitochondrial citrate transmembrane transport GO:0006843 IDA
Subcellular Localization
    nucleus GO:0005634 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial inner membrane GO:0005743 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SLC25A1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SLC25A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SPRTN  
Affinity Capture-MS Homo sapiens
3 MCM2 4171
Affinity Capture-MS Homo sapiens
4 COIL  
Proximity Label-MS Homo sapiens
5 VAPA 9218
Affinity Capture-MS Homo sapiens
6 TUBB3 10381
Affinity Capture-MS Homo sapiens
7 ACOT9 23597
Co-fractionation Homo sapiens
8 TARDBP 23435
Affinity Capture-MS Homo sapiens
9 RPL9 6133
Co-fractionation Homo sapiens
10 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
11 CAND1 55832
Affinity Capture-MS Homo sapiens
12 NDUFA4 4697
Affinity Capture-MS Homo sapiens
13 ASXL1  
Affinity Capture-MS Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 KIAA1429 25962
Affinity Capture-MS Homo sapiens
16 CAPZB 832
Affinity Capture-MS Homo sapiens
17 RUVBL1 8607
Affinity Capture-MS Homo sapiens
18 B3GNT2 10678
Affinity Capture-MS Homo sapiens
19 DYRK1A 1859
Affinity Capture-MS Homo sapiens
20 COQ9  
Affinity Capture-MS Homo sapiens
21 PTPMT1 114971
Affinity Capture-MS Homo sapiens
22 FRA10AC1 118924
Cross-Linking-MS (XL-MS) Homo sapiens
23 FBXW7  
Affinity Capture-MS Homo sapiens
24 VKORC1 79001
Two-hybrid Homo sapiens
25 CHCHD10  
Affinity Capture-MS Homo sapiens
26 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 LOC101929876 101929876
Co-fractionation Homo sapiens
28 ATG16L1 55054
Affinity Capture-MS Homo sapiens
29 MECP2 4204
Affinity Capture-MS Homo sapiens
30 HSPA8 3312
Affinity Capture-MS Homo sapiens
31 ADSL 158
Affinity Capture-MS Homo sapiens
32 OTUD1 220213
Affinity Capture-MS Homo sapiens
33 NF2 4771
Affinity Capture-MS Homo sapiens
34 PHF20L1  
Cross-Linking-MS (XL-MS) Homo sapiens
35 EMD 2010
Proximity Label-MS Homo sapiens
36 ARAF 369
Affinity Capture-MS Homo sapiens
37 PELO 53918
Affinity Capture-MS Homo sapiens
38 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 FAM162A 26355
Co-fractionation Homo sapiens
40 C12orf49  
Negative Genetic Homo sapiens
41 ESRRB  
Affinity Capture-MS Homo sapiens
42 CHMP4B 128866
Affinity Capture-MS Homo sapiens
43 SREBF2 6721
Negative Genetic Homo sapiens
44 SLC25A20 788
Co-fractionation Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 CTNNB1 1499
Affinity Capture-MS Homo sapiens
47 ASB10  
Affinity Capture-MS Homo sapiens
48 ENO1 2023
Affinity Capture-MS Homo sapiens
49 PCSK9 255738
Affinity Capture-MS Homo sapiens
50 GMPS 8833
Affinity Capture-MS Homo sapiens
51 HDAC5 10014
Affinity Capture-MS Homo sapiens
52 KRBOX4  
Affinity Capture-MS Homo sapiens
53 CISD3 284106
Affinity Capture-MS Homo sapiens
54 IMMT 10989
Co-fractionation Homo sapiens
55 C15orf48  
Affinity Capture-MS Homo sapiens
56 TMPO 7112
Affinity Capture-MS Homo sapiens
57 TUBB 203068
Affinity Capture-MS Homo sapiens
58 ASS1 445
Affinity Capture-MS Homo sapiens
59 AMBRA1  
Affinity Capture-MS Homo sapiens
60 RLIM 51132
Affinity Capture-MS Homo sapiens
61 SREBF1 6720
Positive Genetic Homo sapiens
62 CD274 29126
Affinity Capture-MS Homo sapiens
63 CUL3 8452
Affinity Capture-MS Homo sapiens
64 FBXO6 26270
Affinity Capture-MS Homo sapiens
65 RIPK4  
Affinity Capture-MS Homo sapiens
66 FN1 2335
Affinity Capture-MS Homo sapiens
67 OCIAD1 54940
Affinity Capture-MS Homo sapiens
68 AURKB 9212
Affinity Capture-MS Homo sapiens
69 AGO4  
Affinity Capture-MS Homo sapiens
70 ATP5J2 9551
Co-fractionation Homo sapiens
71 PCBP1 5093
Affinity Capture-MS Homo sapiens
72 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 RPA3 6119
Proximity Label-MS Homo sapiens
74 YWHAQ 10971
Affinity Capture-MS Homo sapiens
75 DDRGK1 65992
Affinity Capture-MS Homo sapiens
76 RHOB 388
Proximity Label-MS Homo sapiens
77 ILK 3611
Affinity Capture-MS Homo sapiens
78 SIK2  
Affinity Capture-MS Homo sapiens
79 LDLR 3949
Negative Genetic Homo sapiens
80 PEX3 8504
Proximity Label-MS Homo sapiens
81 CBX4  
Affinity Capture-MS Homo sapiens
82 PPT1 5538
Affinity Capture-MS Homo sapiens
83 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
84 AATK  
Affinity Capture-MS Homo sapiens
85 SPTAN1 6709
Cross-Linking-MS (XL-MS) Homo sapiens
86 Tubg1 103733
Affinity Capture-MS Mus musculus
87 PARP1 142
Proximity Label-MS Homo sapiens
88 BKRF1  
Affinity Capture-MS
89 ICT1 3396
Affinity Capture-MS Homo sapiens
90 ZNF746  
Affinity Capture-MS Homo sapiens
91 Itgb1 16412
Affinity Capture-MS Mus musculus
92 ASB17  
Affinity Capture-MS Homo sapiens
93 RPS4X 6191
Co-fractionation Homo sapiens
94 SQSTM1 8878
Proximity Label-MS Homo sapiens
95 ERLIN2 11160
Affinity Capture-MS Homo sapiens
96 BMPR1A 657
Affinity Capture-MS Homo sapiens
97 MLH1 4292
Affinity Capture-MS Homo sapiens
98 OTUB1 55611
Affinity Capture-MS Homo sapiens
99 RPS5 6193
Co-fractionation Homo sapiens
100 RPS26 6231
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
101 UFL1 23376
Affinity Capture-MS Homo sapiens
102 MAGED1 9500
Affinity Capture-MS Homo sapiens
103 NCL 4691
Affinity Capture-MS Homo sapiens
104 CDK4 1019
Affinity Capture-MS Homo sapiens
105 CDK9 1025
Affinity Capture-MS Homo sapiens
106 ATP1A1 476
Affinity Capture-MS Homo sapiens
107 SERBP1 26135
Affinity Capture-MS Homo sapiens
108 C9orf72  
Affinity Capture-MS Homo sapiens
109 RC3H1 149041
Affinity Capture-MS Homo sapiens
110 LIMK2 3985
Affinity Capture-MS Homo sapiens
111 RBCK1  
Affinity Capture-MS Homo sapiens
112 ASB14  
Affinity Capture-MS Homo sapiens
113 MGST3 4259
Co-fractionation Homo sapiens
114 RANBP2 5903
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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