Gene description for SLC25A20
Gene name solute carrier family 25 (carnitine/acylcarnitine translocase), member 20
Gene symbol SLC25A20
Other names/aliases CAC
CACT
Species Homo sapiens
 Database cross references - SLC25A20
ExoCarta ExoCarta_788
Vesiclepedia VP_788
Entrez Gene 788
HGNC 1421
MIM 613698
UniProt O43772  
 SLC25A20 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SLC25A20
Molecular Function
    protein binding GO:0005515 IPI
    acyl carnitine transmembrane transporter activity GO:0015227 EXP
    acyl carnitine transmembrane transporter activity GO:0015227 IBA
    acyl carnitine transmembrane transporter activity GO:0015227 ISS
Biological Process
    in utero embryonic development GO:0001701 IEA
    mitochondrial transport GO:0006839 IBA
    carnitine shuttle GO:0006853 TAS
    carnitine transmembrane transport GO:1902603 IBA
    carnitine transmembrane transport GO:1902603 ISS
    acyl carnitine transmembrane transport GO:1902616 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial envelope GO:0005740 IBA
    mitochondrial inner membrane GO:0005743 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified SLC25A20 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SLC25A20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TOMM40 10452
Co-fractionation Homo sapiens
2 RAMP3  
Affinity Capture-MS Homo sapiens
3 HNRNPD 3184
Affinity Capture-MS Homo sapiens
4 KLK15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PREB 10113
Co-fractionation Homo sapiens
6 SLC25A6 293
Co-fractionation Homo sapiens
7 HADHA 3030
Co-fractionation Homo sapiens
8 RAB1A 5861
Co-fractionation Homo sapiens
9 SLC25A3 5250
Co-fractionation Homo sapiens
10 CD3D 915
Affinity Capture-MS Homo sapiens
11 ATP5L 10632
Co-fractionation Homo sapiens
12 HSPA8 3312
Co-fractionation Homo sapiens
13 ACOT9 23597
Co-fractionation Homo sapiens
14 LRRC25  
Affinity Capture-MS Homo sapiens
15 ARL15 54622
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TMEM205 374882
Co-fractionation Homo sapiens
17 MMP26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ALDH2 217
Co-fractionation Homo sapiens
19 MGARP  
Affinity Capture-MS Homo sapiens
20 CRAT 1384
Co-fractionation Homo sapiens
21 SLC25A1 6576
Co-fractionation Homo sapiens
22 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
23 SFXN1 94081
Co-fractionation Homo sapiens
24 Wash  
Affinity Capture-MS Mus musculus
25 CPQ 10404
Affinity Capture-MS Homo sapiens
26 POLR3E 55718
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PDE2A  
Affinity Capture-MS Homo sapiens
28 ESR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 KLK1 3816
Affinity Capture-MS Homo sapiens
30 Tbc1d7  
Affinity Capture-MS Mus musculus
31 LETM1 3954
Co-fractionation Homo sapiens
32 SGTB  
Affinity Capture-MS Homo sapiens
33 ATP5J2 9551
Co-fractionation Homo sapiens
34 CTDSP2 10106
Affinity Capture-MS Homo sapiens
35 FIS1 51024
Affinity Capture-MS Homo sapiens
36 KDM1A 23028
Affinity Capture-Western Homo sapiens
37 USMG5 84833
Co-fractionation Homo sapiens
38 OGDH 4967
Co-fractionation Homo sapiens
39 ACOX1 51
Co-fractionation Homo sapiens
40 HSPA2 3306
Co-fractionation Homo sapiens
41 TOMM5  
Co-fractionation Homo sapiens
42 RAB7A 7879
Co-fractionation Homo sapiens
43 CYB5R1 51706
Co-fractionation Homo sapiens
44 CYB5R3 1727
Co-fractionation Homo sapiens
45 NIT1 4817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ACTB 60
Co-fractionation Homo sapiens
47 SERBP1 26135
Affinity Capture-MS Homo sapiens
48 OCIAD2 132299
Co-fractionation Homo sapiens
49 CLEC2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 GLMP 112770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 VDAC1 7416
Co-fractionation Homo sapiens
52 ISLR 3671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 MGST1 4257
Co-fractionation Homo sapiens
54 PRDX2 7001
Co-fractionation Homo sapiens
55 MRAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 GNG5 2787
Co-fractionation Homo sapiens
57 VDAC2 7417
Co-fractionation Homo sapiens
58 MGST3 4259
Co-fractionation Homo sapiens
59 SCCPDH 51097
Co-fractionation Homo sapiens
60 VDAC3 7419
Co-fractionation Homo sapiens
61 SLC25A5 292
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which SLC25A20 is involved
PathwayEvidenceSource
Carnitine shuttle TAS Reactome
Fatty acid metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome





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