Gene description for MGST1
Gene name microsomal glutathione S-transferase 1
Gene symbol MGST1
Other names/aliases GST12
MGST
MGST-I
Species Homo sapiens
 Database cross references - MGST1
ExoCarta ExoCarta_4257
Entrez Gene 4257
HGNC 7061
MIM 138330
UniProt P10620  
 MGST1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MGST1
Molecular Function
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IEA
    glutathione peroxidase activity GO:0004602 IDA
    glutathione binding GO:0043295 IEA
    glutathione transferase activity GO:0004364 IDA
Biological Process
    cellular response to lipid hydroperoxide GO:0071449 IDA
    protein homotrimerization GO:0070207 ISS
    response to lipopolysaccharide GO:0032496 IEA
    response to organonitrogen compound GO:0010243 IEA
    response to drug GO:0042493 IEA
    small molecule metabolic process GO:0044281 TAS
    xenobiotic metabolic process GO:0006805 TAS
    oxidation-reduction process GO:0055114 IDA
    glutathione metabolic process GO:0006749 IEA
    glutathione derivative biosynthetic process GO:1901687 TAS
    Leydig cell differentiation GO:0033327 IEA
Subcellular Localization
    mitochondrial outer membrane GO:0005741 IEA
    endoplasmic reticulum GO:0005783 ISS
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IEA
    peroxisomal membrane GO:0005778 ISS
    apical part of cell GO:0045177 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
    integral component of membrane GO:0016021 ISS
    nucleus GO:0005634 IEA
 Experiment description of studies that identified MGST1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MGST1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MGST1 is involved
PathwayEvidenceSource
Aflatoxin activation and detoxification TAS Reactome
Glutathione conjugation TAS Reactome





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