Gene description for MGST1
Gene name microsomal glutathione S-transferase 1
Gene symbol MGST1
Other names/aliases GST12
MGST
MGST-I
Species Homo sapiens
 Database cross references - MGST1
ExoCarta ExoCarta_4257
Vesiclepedia VP_4257
Entrez Gene 4257
HGNC 7061
MIM 138330
UniProt P10620  
 MGST1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MGST1
Molecular Function
    glutathione transferase activity GO:0004364 IBA
    glutathione transferase activity GO:0004364 IDA
    glutathione peroxidase activity GO:0004602 IDA
    protein binding GO:0005515 IPI
Biological Process
    glutathione transport GO:0034635 IDA
    cellular response to lipid hydroperoxide GO:0071449 IDA
    cellular oxidant detoxification GO:0098869 IEA
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IEA
    peroxisomal membrane GO:0005778 ISS
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 ISS
    azurophil granule membrane GO:0035577 TAS
 Experiment description of studies that identified MGST1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MGST1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAMP3  
Affinity Capture-MS Homo sapiens
2 RAB1A 5861
Co-fractionation Homo sapiens
3 VAPA 9218
Co-fractionation Homo sapiens
4 ACOT9 23597
Co-fractionation Homo sapiens
5 DYNC1I2 1781
Proximity Label-MS Homo sapiens
6 RPA2 6118
Proximity Label-MS Homo sapiens
7 ARL8B 55207
Co-fractionation Homo sapiens
8 SLC25A24 29957
Co-fractionation Homo sapiens
9 DNAJB6 10049
Affinity Capture-MS Homo sapiens
10 KIAA1429 25962
Affinity Capture-MS Homo sapiens
11 OGDH 4967
Co-fractionation Homo sapiens
12 PRDX3 10935
Co-fractionation Homo sapiens
13 COQ9  
Affinity Capture-MS Homo sapiens
14 COX7A2L 9167
Co-fractionation Homo sapiens
15 CYB5R1 51706
Co-fractionation Homo sapiens
16 COA3 28958
Co-fractionation Homo sapiens
17 DDOST 1650
Co-fractionation Homo sapiens
18 PGRMC2 10424
Co-fractionation Homo sapiens
19 VAPB 9217
Co-fractionation Homo sapiens
20 CCDC47 57003
Affinity Capture-MS Homo sapiens
21 PHB 5245
Co-fractionation Homo sapiens
22 MECP2 4204
Affinity Capture-MS Homo sapiens
23 PCYOX1 51449
Co-fractionation Homo sapiens
24 MLEC 9761
Co-fractionation Homo sapiens
25 SLC25A3 5250
Co-fractionation Homo sapiens
26 LAMTOR1 55004
Co-fractionation Homo sapiens
27 STOML2 30968
Co-fractionation Homo sapiens
28 CYB5R3 1727
Co-fractionation Homo sapiens
29 HS2ST1 9653
Affinity Capture-MS Homo sapiens
30 ARL6IP5 10550
Co-fractionation Homo sapiens
31 LYZL6  
Affinity Capture-MS Homo sapiens
32 ESRRB  
Affinity Capture-MS Homo sapiens
33 CD300C  
Affinity Capture-MS Homo sapiens
34 USMG5 84833
Co-fractionation Homo sapiens
35 SLC25A20 788
Co-fractionation Homo sapiens
36 MOGS 7841
Co-fractionation Homo sapiens
37 OCIAD2 132299
Co-fractionation Homo sapiens
38 RPN1 6184
Co-fractionation Homo sapiens
39 AGPS 8540
Co-fractionation Homo sapiens
40 GNG5 2787
Co-fractionation Homo sapiens
41 SEC61B 10952
Co-fractionation Homo sapiens
42 ATP5B 506
Co-fractionation Homo sapiens
43 SSR4 6748
Co-fractionation Homo sapiens
44 RAB6A 5870
Co-fractionation Homo sapiens
45 HADHB 3032
Co-fractionation Homo sapiens
46 HADHA 3030
Co-fractionation Homo sapiens
47 RPN2 6185
Co-fractionation Homo sapiens
48 GNB4 59345
Co-fractionation Homo sapiens
49 UQCRC2 7385
Co-fractionation Homo sapiens
50 HMOX2 3163
Co-fractionation Homo sapiens
51 EEF2 1938
Co-fractionation Homo sapiens
52 ATP5F1 515
Co-fractionation Homo sapiens
53 EGFR 1956
Affinity Capture-MS Homo sapiens
54 MORF4L1  
Affinity Capture-MS Homo sapiens
55 ARMC6 93436
Affinity Capture-MS Homo sapiens
56 RPA3 6119
Proximity Label-MS Homo sapiens
57 GNB2 2783
Co-fractionation Homo sapiens
58 NDUFA3  
Co-fractionation Homo sapiens
59 GALNT2 2590
Co-fractionation Homo sapiens
60 NELFB 25920
Reconstituted Complex Homo sapiens
61 ATP6AP1 537
Co-fractionation Homo sapiens
62 SCCPDH 51097
Co-fractionation Homo sapiens
63 PHB2 11331
Co-fractionation Homo sapiens
64 TMED10 10972
Co-fractionation Homo sapiens
65 LTC4S  
Two-hybrid Homo sapiens
66 ATP5L 10632
Co-fractionation Homo sapiens
67 OPALIN  
Affinity Capture-MS Homo sapiens
68 BST2 684
Co-fractionation Homo sapiens
69 SQSTM1 8878
Proximity Label-MS Homo sapiens
70 PDHB 5162
Co-fractionation Homo sapiens
71 SFTPA2  
Affinity Capture-MS Homo sapiens
72 GNB1 2782
Co-fractionation Homo sapiens
73 SFXN1 94081
Co-fractionation Homo sapiens
74 ATP6V0C 527
Co-fractionation Homo sapiens
75 RAB2A 5862
Co-fractionation Homo sapiens
76 SPCS1 28972
Co-fractionation Homo sapiens
77 RAB7A 7879
Co-fractionation Homo sapiens
78 PINK1  
Affinity Capture-MS Homo sapiens
79 TMEM109 79073
Co-fractionation Homo sapiens
80 COX7A2 1347
Co-fractionation Homo sapiens
81 SERBP1 26135
Affinity Capture-MS Homo sapiens
82 RAB5C 5878
Co-fractionation Homo sapiens
83 RAC1 5879
Co-fractionation Homo sapiens
84 VDAC3 7419
Co-fractionation Homo sapiens
85 MTCH2 23788
Co-fractionation Homo sapiens
86 SLC3A2 6520
Co-fractionation Homo sapiens
View the network image/svg+xml



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