Gene description for NELFB
Gene name negative elongation factor complex member B
Gene symbol NELFB
Other names/aliases COBRA1
NELF-B
Species Homo sapiens
 Database cross references - NELFB
ExoCarta ExoCarta_25920
Entrez Gene 25920
HGNC 24324
MIM 611180
UniProt Q8WX92  
 NELFB identified in exosomes derived from the following tissue/cell type
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for NELFB
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    viral process GO:0016032 TAS
    gene expression GO:0010467 TAS
    positive regulation of viral transcription GO:0050434 TAS
    negative regulation of transcription, DNA-templated GO:0045892 IEA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
Subcellular Localization
    NELF complex GO:0032021 IDA
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified NELFB in exosomes
1
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NELFB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NELFCD 51497
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 ESR1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 JUN 3725
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 BRCA1 672
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
5 FOS 2353
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 RDBP  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 WHSC2  
Reconstituted Complex Homo sapiens
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