Gene description for CD274
Gene name CD274 molecule
Gene symbol CD274
Other names/aliases B7-H
B7H1
PD-L1
PDCD1L1
PDCD1LG1
PDL1
Species Homo sapiens
 Database cross references - CD274
ExoCarta ExoCarta_29126
Vesiclepedia VP_29126
Entrez Gene 29126
HGNC 17635
MIM 605402
UniProt Q9NZQ7  
 CD274 identified in exosomes derived from the following tissue/cell type
Breast cancer cells 30089911    
Breast cancer cells 30089911    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 30089911    
Lung cancer cells 30089911    
Lung cancer cells 30089911    
Lung cancer cells 30089911    
Lung cancer cells 30089911    
Lung cancer cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Melanoma cells 30089911    
Plasma 17076679    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Plasma 30089911    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for CD274
Molecular Function
    transcription coactivator activity GO:0003713 IDA
    protein binding GO:0005515 IPI
    receptor ligand activity GO:0048018 IDA
Biological Process
    adaptive immune response GO:0002250 IEA
    negative regulation of T cell mediated immune response to tumor cell GO:0002841 IDA
    negative regulation of T cell mediated immune response to tumor cell GO:0002841 IMP
    immune response GO:0006955 IBA
    immune response GO:0006955 IDA
    signal transduction GO:0007165 IDA
    cell surface receptor signaling pathway GO:0007166 IBA
    cell surface receptor signaling pathway GO:0007166 IDA
    positive regulation of cell migration GO:0030335 IEA
    T cell costimulation GO:0031295 IBA
    T cell costimulation GO:0031295 IDA
    negative regulation of type II interferon production GO:0032689 IMP
    negative regulation of interleukin-10 production GO:0032693 IMP
    positive regulation of interleukin-10 production GO:0032733 IDA
    response to cytokine GO:0034097 IDA
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 IDA
    positive regulation of T cell proliferation GO:0042102 IBA
    positive regulation of T cell proliferation GO:0042102 TAS
    negative regulation of T cell proliferation GO:0042130 IBA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    regulation of activated T cell proliferation GO:0046006 IMP
    negative regulation of activated T cell proliferation GO:0046007 IMP
    regulation of T cell apoptotic process GO:0070232 IMP
    cellular response to lipopolysaccharide GO:0071222 IBA
    negative regulation of tumor necrosis factor superfamily cytokine production GO:1903556 IMP
    regulation of activated CD4-positive, alpha-beta T cell apoptotic process GO:1905399 IDA
    positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process GO:1905404 IDA
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 IDA
    negative regulation of CD8-positive, alpha-beta T cell activation GO:2001186 IDA
    negative regulation of CD8-positive, alpha-beta T cell activation GO:2001186 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    actin cytoskeleton GO:0015629 IDA
    early endosome membrane GO:0031901 IDA
    recycling endosome membrane GO:0055038 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CD274 in exosomes
1
Experiment ID 989
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 990
MISEV standards
✘ Biophysical techniques
✔
CD63|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 542
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299-fraction 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 543
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299-fraction 4
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 544
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299-fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 546
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|GAPDH
Enriched markers
✔
GOLGA2
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
7
Experiment ID 547
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 548
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 549
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 550
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 551
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 554
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
13
Experiment ID 555
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
14
Experiment ID 556
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
15
Experiment ID 557
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975-fraction 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
16
Experiment ID 558
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975-fraction 4
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
17
Experiment ID 559
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975-fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
18
Experiment ID 560
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
19
Experiment ID 561
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
20
Experiment ID 562
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 563
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 564
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
23
Experiment ID 565
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
24
Experiment ID 566
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
25
Experiment ID 567
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
26
Experiment ID 568
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
27
Experiment ID 986
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
28
Experiment ID 987
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Lung cancer cells
Sample name H358
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
29
Experiment ID 988
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Lung cancer cells
Sample name H1264
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
30
Experiment ID 991
MISEV standards
✘ Biophysical techniques
✔
CD63|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
31
Experiment ID 992
MISEV standards
✘ Biophysical techniques
✔
CD63|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Lung cancer cells
Sample name H358
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
32
Experiment ID 993
MISEV standards
✘ Biophysical techniques
✔
CD63|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Lung cancer cells
Sample name H1264
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
33
Experiment ID 955
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
Reverse phase protein array
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name A375
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Reverse phase protein array
34
Experiment ID 956
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
Reverse phase protein array
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name UACC-903
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Reverse phase protein array
35
Experiment ID 957
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Reverse phase protein array
Immuno-EM
ELISA
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM9
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Reverse phase protein array
Immuno-EM
ELISA
36
Experiment ID 958
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
Reverse phase protein array
ELISA
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM164
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Reverse phase protein array
ELISA
37
Experiment ID 959
MISEV standards
✘ Biophysical techniques
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Reverse phase protein array
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM1552C
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Reverse phase protein array
38
Experiment ID 960
MISEV standards
✘ Biophysical techniques
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Reverse phase protein array
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM35
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Reverse phase protein array
39
Experiment ID 961
MISEV standards
✘ Biophysical techniques
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Reverse phase protein array
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM793
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Reverse phase protein array
40
Experiment ID 962
MISEV standards
✘ Biophysical techniques
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Reverse phase protein array
PubMed ID 30089911    
Organism Homo sapiens
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name WM902B
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Reverse phase protein array
41
Experiment ID 963
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101