Gene description for CD274
Gene name CD274 molecule
Gene symbol CD274
Other names/aliases B7-H
B7H1
PD-L1
PDCD1L1
PDCD1LG1
PDL1
Species Homo sapiens
 Database cross references - CD274
ExoCarta ExoCarta_29126
Entrez Gene 29126
HGNC 17635
MIM 605402
UniProt Q9NZQ7  
 CD274 identified in exosomes derived from the following tissue/cell type
Plasma 17076679    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for CD274
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    toxin transport GO:1901998 IEA
    T cell costimulation GO:0031295 TAS
    negative regulation of interleukin-10 production GO:0032693 IMP
    regulation of activated T cell proliferation GO:0046006 IMP
    negative regulation of activated T cell proliferation GO:0046007 IMP
    positive regulation of T cell proliferation GO:0042102 TAS
    negative regulation of tumor necrosis factor superfamily cytokine production GO:1903556 IMP
    immune response GO:0006955 IDA
    regulation of T cell apoptotic process GO:0070232 IMP
    cell surface receptor signaling pathway GO:0007166 IDA
    positive regulation of interleukin-10 secretion GO:2001181 IDA
    signal transduction GO:0007165 IDA
    negative regulation of interferon-gamma production GO:0032689 IMP
Subcellular Localization
    endomembrane system GO:0012505 IEA
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 IEA
    extracellular exosome GO:0070062 IDA
    external side of plasma membrane GO:0009897 IEA
 Experiment description of studies that identified CD274 in exosomes
1
Experiment ID 43
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD83
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17076679    
Organism Homo sapiens
Experiment description Specific isolation of placenta-derived exosomes from the circulation of pregnant women and their immunoregulatory consequences.
Authors Sabapatha A, Gercel-Taylor C, Taylor DD
Journal name AJRI
Publication year 2006
Sample Plasma
Sample name Plasma - Pregnant and not-pregnant women
Isolation/purification methods Size exclusion
Ultracentrifugation
Immunobeads (CD3, CD19, CD56, CD83, PLAP)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 193
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
PHB
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 194
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 195
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CD274
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which CD274 is involved
PathwayEvidenceSource
PD-1 signaling TAS Reactome





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