Gene description for THADA
Gene name thyroid adenoma associated
Gene symbol THADA
Other names/aliases GITA
Species Homo sapiens
 Database cross references - THADA
ExoCarta ExoCarta_63892
Vesiclepedia VP_63892
Entrez Gene 63892
HGNC 19217
MIM 611800
UniProt Q6YHU6  
 THADA identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for THADA
Molecular Function
    protein binding GO:0005515 IPI
    enzyme regulator activity GO:0030234 IGI
Biological Process
    tRNA nucleoside ribose methylation GO:0002128 IGI
    tRNA methylation GO:0030488 IBA
    tRNA methylation GO:0030488 IGI
    negative regulation of endoplasmic reticulum calcium ion concentration GO:0032471 IMP
    lipid homeostasis GO:0055088 IGI
    adaptive thermogenesis GO:1990845 ISS
Subcellular Localization
    cytoplasmic side of endoplasmic reticulum membrane GO:0098554 ISS
 Experiment description of studies that identified THADA in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for THADA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYPD3 27076
Affinity Capture-MS Homo sapiens
2 HCST  
Affinity Capture-MS Homo sapiens
3 TRIM66  
Affinity Capture-MS Homo sapiens
4 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 FPR1  
Affinity Capture-MS Homo sapiens
6 TYROBP  
Affinity Capture-MS Homo sapiens
7 EDNRB 1910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 C5AR1  
Affinity Capture-MS Homo sapiens
9 APEX1 328
Affinity Capture-RNA Homo sapiens
10 GPR17 2840
Affinity Capture-MS Homo sapiens
11 C11orf87  
Affinity Capture-MS Homo sapiens
12 FAM174A 345757
Affinity Capture-MS Homo sapiens
13 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MLST8 64223
Affinity Capture-MS Homo sapiens
15 USHBP1  
Two-hybrid Homo sapiens
16 TACSTD2 4070
Affinity Capture-MS Homo sapiens
17 ITFG3 83986
Affinity Capture-MS Homo sapiens
18 F2RL1  
Affinity Capture-MS Homo sapiens
19 SEC23A 10484
Co-fractionation Homo sapiens
20 CA14 23632
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TES 26136
Co-fractionation Homo sapiens
22 AHCYL1 10768
Co-fractionation Homo sapiens
23 PROKR1  
Affinity Capture-MS Homo sapiens
24 PFN1 5216
Proximity Label-MS Homo sapiens
25 VSIG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 KRT8 3856
Proximity Label-MS Homo sapiens
27 CD70 970
Affinity Capture-MS Homo sapiens
28 C19orf38  
Affinity Capture-MS Homo sapiens
29 P2RY8  
Affinity Capture-MS Homo sapiens
30 ILVBL 10994
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 COMTD1 118881
Affinity Capture-MS Homo sapiens
32 POU6F2  
Two-hybrid Homo sapiens
33 S1PR1 1901
Affinity Capture-MS Homo sapiens
34 GPR182  
Affinity Capture-MS Homo sapiens
35 DNAJA1 3301
Proximity Label-MS Homo sapiens
36 DNM1L 10059
Proximity Label-MS Homo sapiens
37 MLNR  
Affinity Capture-MS Homo sapiens
38 IGBP1 3476
Co-fractionation Homo sapiens
39 CD40 958
Affinity Capture-MS Homo sapiens
40 P2RY2 5029
Affinity Capture-MS Homo sapiens
41 NTRK1 4914
Affinity Capture-MS Homo sapiens
42 VIPR2  
Affinity Capture-MS Homo sapiens
43 CYB5B 80777
Affinity Capture-MS Homo sapiens
44 EFNA4  
Affinity Capture-MS Homo sapiens
45 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 BTNL9  
Affinity Capture-MS Homo sapiens
48 NUBP2 10101
Co-fractionation Homo sapiens
49 CD83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 SRXN1 140809
Co-fractionation Homo sapiens
51 FZD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 LRRC25  
Affinity Capture-MS Homo sapiens
53 NPTN 27020
Affinity Capture-MS Homo sapiens
54 CD274 29126
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CLEC4A  
Affinity Capture-MS Homo sapiens
58 EGFR 1956
Affinity Capture-MS Homo sapiens
59 AQP3  
Affinity Capture-MS Homo sapiens
60 OPRL1 4987
Affinity Capture-MS Homo sapiens
61 SEC24A 10802
Co-fractionation Homo sapiens
62 XPO6 23214
Affinity Capture-MS Homo sapiens
63 CD226 10666
Affinity Capture-MS Homo sapiens
64 XPO4 64328
Affinity Capture-MS Homo sapiens
65 UBA2 10054
Co-fractionation Homo sapiens
66 C3orf18  
Affinity Capture-MS Homo sapiens
67 GPR45  
Affinity Capture-MS Homo sapiens
68 MTOR 2475
Affinity Capture-MS Homo sapiens
69 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 P2RY10  
Affinity Capture-MS Homo sapiens
71 OCIAD1 54940
Affinity Capture-MS Homo sapiens
72 GPR84  
Affinity Capture-MS Homo sapiens
73 NAA10 8260
Affinity Capture-MS Homo sapiens
74 AVPR2  
Affinity Capture-MS Homo sapiens
75 GYPA  
Affinity Capture-MS Homo sapiens
76 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
77 ALDH3A2 224
Affinity Capture-MS Homo sapiens
78 CCKBR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 VASN 114990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 SIN3A  
Co-fractionation Homo sapiens
81 PSAT1 29968
Co-fractionation Homo sapiens
82 OPALIN  
Affinity Capture-MS Homo sapiens
83 MFSD4  
Affinity Capture-MS Homo sapiens
84 SULT1A1 6817
Co-fractionation Homo sapiens
85 CHRM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 EFNB1 1947
Affinity Capture-MS Homo sapiens
87 MGARP  
Affinity Capture-MS Homo sapiens
88 CD80 941
Affinity Capture-MS Homo sapiens
89 SPN 6693
Affinity Capture-MS Homo sapiens
90 SSSCA1 10534
Co-fractionation Homo sapiens
91 TPST2 8459
Affinity Capture-MS Homo sapiens
92 VIPR1  
Affinity Capture-MS Homo sapiens
93 FXYD3 5349
Affinity Capture-MS Homo sapiens
94 STAM 8027
Co-fractionation Homo sapiens
95 S1PR4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which THADA is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA modification in the nucleus and cytosol TAS Reactome
tRNA processing TAS Reactome





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