Gene description for CD226
Gene name CD226 molecule
Gene symbol CD226
Other names/aliases DNAM-1
DNAM1
PTA1
TLiSA1
Species Homo sapiens
 Database cross references - CD226
ExoCarta ExoCarta_10666
Vesiclepedia VP_10666
Entrez Gene 10666
HGNC 16961
MIM 605397
UniProt Q15762  
 CD226 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for CD226
Molecular Function
    protein binding GO:0005515 IPI
    cell adhesion molecule binding GO:0050839 IPI
    integrin binding GO:0005178 IPI
    protein kinase binding GO:0019901 IPI
Biological Process
    cell adhesion GO:0007155 IEA
    positive regulation of mast cell activation GO:0033005 IDA
    signal transduction GO:0007165 TAS
    cell recognition GO:0008037 TAS
    positive regulation of Fc receptor mediated stimulatory signaling pathway GO:0060369 IDA
    positive regulation of natural killer cell mediated cytotoxicity GO:0045954 IMP
    regulation of immune response GO:0050776 TAS
    positive regulation of immunoglobulin mediated immune response GO:0002891 IDA
    cytokine production GO:0001816 IEA
    positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target GO:0002860 IMP
Subcellular Localization
    membrane raft GO:0045121 TAS
    integral component of plasma membrane GO:0005887 TAS
    cell surface GO:0009986 IDA
    external side of plasma membrane GO:0009897 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified CD226 in exosomes
1
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CD226
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB2 3689
Invivo Homo sapiens
2 FYN 2534
Invivo Homo sapiens
3 PVRL2 5819
Reconstituted Complex Homo sapiens
4 PVR 5817
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CD226 is involved
PathwayEvidenceSource
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell TAS Reactome





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