Gene description for PVRL2
Gene name poliovirus receptor-related 2 (herpesvirus entry mediator B)
Gene symbol PVRL2
Other names/aliases CD112
HVEB
PRR2
PVRR2
Species Homo sapiens
 Database cross references - PVRL2
ExoCarta ExoCarta_5819
Entrez Gene 5819
HGNC 9707
MIM 600798
UniProt Q92692  
 PVRL2 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
 Gene ontology annotations for PVRL2
Molecular Function
    coreceptor activity GO:0015026 TAS
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IPI
    cell adhesion molecule binding GO:0050839 IPI
    virus receptor activity GO:0001618 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    establishment of mitochondrion localization GO:0051654 IEA
    susceptibility to natural killer cell mediated cytotoxicity GO:0042271 IMP
    adherens junction organization GO:0034332 TAS
    fusion of virus membrane with host plasma membrane GO:0019064 IDA
    cell-cell junction organization GO:0045216 TAS
    positive regulation of natural killer cell mediated cytotoxicity GO:0045954 IMP
    acrosome assembly GO:0001675 IEA
    sperm mitochondrion organization GO:0030382 IEA
    fertilization GO:0009566 IEA
    regulation of viral entry into host cell GO:0046596 IDA
    signal transduction GO:0007165 TAS
    susceptibility to T cell mediated cytotoxicity GO:0060370 IDA
    spermatid development GO:0007286 ISS
    adhesion of symbiont to host GO:0044406 IDA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IDA
    cell junction assembly GO:0034329 TAS
    positive regulation of immunoglobulin mediated immune response GO:0002891 IMP
    coreceptor-mediated virion attachment to host cell GO:0046814 IDA
    cilium organization GO:0044782 IEA
    positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target GO:0002860 IMP
    cytoskeleton organization GO:0007010 IEA
    cell part morphogenesis GO:0032990 IEA
    regulation of immune response GO:0050776 TAS
    spermatid nucleus differentiation GO:0007289 IEA
    positive regulation of mast cell activation GO:0033005 IMP
Subcellular Localization
    cell-cell junction GO:0005911 IDA
    integral component of membrane GO:0016021 IDA
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 TAS
    zonula adherens GO:0005915 ISS
    cell surface GO:0009986 IDA
    focal adhesion GO:0005925 IDA
 Experiment description of studies that identified PVRL2 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for PVRL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MLLT4 4301
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
2 PVRL2 5819
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 CD226 10666
Reconstituted Complex Homo sapiens
4 MDFI  
Two-hybrid Homo sapiens
5 ATP5J2  
Two-hybrid Homo sapiens
View the network image/svg+xml



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