Experiment description of studies that identified MLLT4 in exosomes

1 Experiment ID 235 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Cytosolic markers ✘ EV Membrane markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 236 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Cytosolic markers ✘ EV Membrane markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 235 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Cytosolic markers ✘ EV Membrane markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

4 Experiment ID 224 ISEV standards ✔ EM|AFM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ CD63|CD81 EV Membrane markers ✔ GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

5 Experiment ID 211 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ cytochrome c|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 212 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ Cytochrome C|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 63 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ AQP2 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for MLLT4

Protein Interactor ExoCarta ID Identification method PubMed Species 1 EPHB6   Two-hybrid Homo sapiens 2 F11R 50848 Invivo Homo sapiens Invitro Homo sapiens Two-hybrid Homo sapiens 3 RAP1GAP 5909 Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 4 YWHAG 7532 Affinity Capture-MS Homo sapiens 5 PVRL2 5819 Affinity Capture-Western Homo sapiens Invivo Homo sapiens Invitro Homo sapiens 6 SORBS1 10580 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 7 NRXN3 9369 Two-hybrid Homo sapiens 8 YWHAB 7529 Affinity Capture-MS Homo sapiens 9 PFN1 5216 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 10 RIT1   Two-hybrid Homo sapiens 11 EPHB2 2048 Two-hybrid Homo sapiens 12 EPHA7   Two-hybrid Homo sapiens 13 TJP1 7082 Invitro Homo sapiens Invivo Homo sapiens 14 PVRL1   Invivo Homo sapiens Invitro Homo sapiens Two-hybrid Homo sapiens 15 HRAS 3265 Two-hybrid Homo sapiens 16 USP9X 8239 Invivo Homo sapiens Invitro Homo sapiens Reconstituted Complex Homo sapiens 17 LOC398149   Reconstituted Complex 8355 18 RIN1   Two-hybrid Homo sapiens 19 PVRL3   Invitro Homo sapiens Invitro Homo sapiens 20 RRAS2 22800 Invitro Homo sapiens Invitro Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 21 SORBS2   Invitro Homo sapiens 22 LMO2   Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 23 Mras   Affinity Capture-Western Mus musculus 24 NRXN1   Two-hybrid Homo sapiens 25 RRAS 6237 Invitro Homo sapiens Two-hybrid Homo sapiens 26 NRAS 4893 Affinity Capture-Western Homo sapiens 27 JAG1 182 Two-hybrid Homo sapiens 28 RAP1A 5906 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens Invivo Homo sapiens Invitro Homo sapiens 29 YWHAQ 10971 Affinity Capture-MS Homo sapiens 30 CTNNA1 1495 Reconstituted Complex Homo sapiens 31 NRXN2   Two-hybrid Homo sapiens 32 EPHB3 2049 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 33 SSX2IP   Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens 34 BCR 613 Biochemical Activity Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 35 RAP2A 5911 Invitro Homo sapiens Two-hybrid Homo sapiens View the network

Pathways in which MLLT4 is involved

No pathways found