Gene ontology annotations for MLLT4
Experiment description of studies that identified MLLT4 in exosomes
1
Experiment ID
235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
2
Experiment ID
236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
3
Experiment ID
235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
4
Experiment ID
224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
5
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
63
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
Protein-protein interactions for MLLT4
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
EPHB6
Two-hybrid
Homo sapiens
2
F11R
50848
Invivo
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
3
RAP1GAP
5909
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
4
YWHAG
7532
Affinity Capture-MS
Homo sapiens
5
PVRL2
5819
Affinity Capture-Western
Homo sapiens
Invivo
Homo sapiens
Invitro
Homo sapiens
6
SORBS1
10580
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
7
NRXN3
9369
Two-hybrid
Homo sapiens
8
YWHAB
7529
Affinity Capture-MS
Homo sapiens
9
PFN1
5216
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
10
RIT1
Two-hybrid
Homo sapiens
11
EPHB2
2048
Two-hybrid
Homo sapiens
12
EPHA7
Two-hybrid
Homo sapiens
13
TJP1
7082
Invitro
Homo sapiens
Invivo
Homo sapiens
14
PVRL1
Invivo
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
15
HRAS
3265
Two-hybrid
Homo sapiens
16
USP9X
8239
Invivo
Homo sapiens
Invitro
Homo sapiens
Reconstituted Complex
Homo sapiens
17
LOC398149
Reconstituted Complex
8355
18
RIN1
Two-hybrid
Homo sapiens
19
PVRL3
Invitro
Homo sapiens
Invitro
Homo sapiens
20
RRAS2
22800
Invitro
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
21
SORBS2
Invitro
Homo sapiens
22
LMO2
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
23
Mras
Affinity Capture-Western
Mus musculus
24
NRXN1
Two-hybrid
Homo sapiens
25
RRAS
6237
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
26
NRAS
4893
Affinity Capture-Western
Homo sapiens
27
JAG1
182
Two-hybrid
Homo sapiens
28
RAP1A
5906
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Invivo
Homo sapiens
Invitro
Homo sapiens
29
YWHAQ
10971
Affinity Capture-MS
Homo sapiens
30
CTNNA1
1495
Reconstituted Complex
Homo sapiens
31
NRXN2
Two-hybrid
Homo sapiens
32
EPHB3
2049
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
33
SSX2IP
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
34
BCR
613
Biochemical Activity
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
35
RAP2A
5911
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which MLLT4 is involved
No pathways found