Gene description for MLLT4
Gene name myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4
Gene symbol MLLT4
Other names/aliases AF6
Species Homo sapiens
 Database cross references - MLLT4
ExoCarta ExoCarta_4301
Entrez Gene 4301
HGNC 7137
MIM 159559
UniProt P55196  
 MLLT4 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for MLLT4
Molecular Function
    protein C-terminus binding GO:0008022 TAS
    protein binding GO:0005515 IPI
    cell adhesion molecule binding GO:0050839 IEA
    Ras GTPase binding GO:0017016 IDA
Biological Process
    signal transduction GO:0007165 IEA
    cell adhesion GO:0007155 IEA
    cell-cell junction organization GO:0045216 TAS
    adherens junction organization GO:0034332 TAS
    cell-cell signaling GO:0007267 TAS
    establishment of endothelial intestinal barrier GO:0090557 IMP
    positive regulation of GTPase activity GO:0043547 IMP
    cell junction assembly GO:0034329 TAS
Subcellular Localization
    plasma membrane GO:0005886 IDA
    cell-cell junction GO:0005911 TAS
    cytosol GO:0005829 TAS
    cell-cell adherens junction GO:0005913 IEA
    cytoplasm GO:0005737 IDA
    cell junction GO:0030054 IDA
    nucleoplasm GO:0005654 IDA
    apical part of cell GO:0045177 IEA
 Experiment description of studies that identified MLLT4 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MLLT4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EPHB6  
Two-hybrid Homo sapiens
2 F11R 50848
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 RAP1GAP 5909
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
4 YWHAG 7532
Affinity Capture-MS Homo sapiens
5 PVRL2 5819
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
6 SORBS1 10580
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 NRXN3 9369
Two-hybrid Homo sapiens
8 YWHAB 7529
Affinity Capture-MS Homo sapiens
9 PFN1 5216
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
10 RIT1  
Two-hybrid Homo sapiens
11 EPHB2 2048
Two-hybrid Homo sapiens
12 EPHA7  
Two-hybrid Homo sapiens
13 TJP1 7082
Invitro Homo sapiens
Invivo Homo sapiens
14 PVRL1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
15 HRAS 3265
Two-hybrid Homo sapiens
16 USP9X 8239
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
17 LOC398149  
Reconstituted Complex 8355
18 RIN1  
Two-hybrid Homo sapiens
19 PVRL3  
Invitro Homo sapiens
Invitro Homo sapiens
20 RRAS2 22800
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 SORBS2  
Invitro Homo sapiens
22 LMO2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 Mras  
Affinity Capture-Western Mus musculus
24 NRXN1  
Two-hybrid Homo sapiens
25 RRAS 6237
Invitro Homo sapiens
Two-hybrid Homo sapiens
26 NRAS 4893
Affinity Capture-Western Homo sapiens
27 JAG1 182
Two-hybrid Homo sapiens
28 RAP1A 5906
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
29 YWHAQ 10971
Affinity Capture-MS Homo sapiens
30 CTNNA1 1495
Reconstituted Complex Homo sapiens
31 NRXN2  
Two-hybrid Homo sapiens
32 EPHB3 2049
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
33 SSX2IP  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
34 BCR 613
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
35 RAP2A 5911
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MLLT4 is involved
No pathways found





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