Gene description for CEP89
Gene name centrosomal protein 89kDa
Gene symbol CEP89
Other names/aliases CCDC123
CEP123
Species Homo sapiens
 Database cross references - CEP89
ExoCarta ExoCarta_84902
Vesiclepedia VP_84902
Entrez Gene 84902
HGNC 25907
MIM 615470
UniProt Q96ST8  
 CEP89 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for CEP89
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitochondrion organization GO:0007005 IBA
    chemical synaptic transmission GO:0007268 IEA
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IMP
    cilium assembly GO:0060271 IMP
    non-motile cilium assembly GO:1905515 IMP
Subcellular Localization
    spindle pole GO:0000922 IDA
    mitochondrial intermembrane space GO:0005758 IDA
    centrosome GO:0005813 IDA
    centriole GO:0005814 IBA
    centriole GO:0005814 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    motile cilium GO:0031514 IEA
    synapse GO:0045202 IEA
    ciliary transition fiber GO:0097539 IBA
    ciliary transition fiber GO:0097539 IDA
    non-motile cilium GO:0097730 IDA
 Experiment description of studies that identified CEP89 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CEP89
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 PTPRG 5793
Proximity Label-MS Homo sapiens
3 LYN 4067
Proximity Label-MS Homo sapiens
4 CALCOCO2  
Affinity Capture-MS Homo sapiens
5 PICK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RAP1GDS1 5910
Affinity Capture-MS Homo sapiens
7 WHAMMP3  
Affinity Capture-MS Homo sapiens
8 OCLN 100506658
Proximity Label-MS Homo sapiens
9 GAR1 54433
Affinity Capture-MS Homo sapiens
10 GJA1 2697
Proximity Label-MS Homo sapiens
11 KRT8 3856
Proximity Label-MS Homo sapiens
12 EPB41L4A 64097
Proximity Label-MS Homo sapiens
13 RAB35 11021
Proximity Label-MS Homo sapiens
14 HSPA12B 116835
Proximity Label-MS Homo sapiens
15 LCK 3932
Proximity Label-MS Homo sapiens
16 YWHAB 7529
Affinity Capture-MS Homo sapiens
17 TOP1MT  
Affinity Capture-MS Homo sapiens
18 CXADR 1525
Proximity Label-MS Homo sapiens
19 MAGEA9  
Affinity Capture-MS Homo sapiens
20 C11orf52 91894
Proximity Label-MS Homo sapiens
21 PMF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 BRK1 55845
Affinity Capture-MS Homo sapiens
23 PRKACA 5566
Proximity Label-MS Homo sapiens
24 MLLT3 4300
Affinity Capture-MS Homo sapiens
25 MLLT4 4301
Proximity Label-MS Homo sapiens
26 PARD3 56288
Proximity Label-MS Homo sapiens
27 DIRAS3  
Proximity Label-MS Homo sapiens
28 MLLT1 4298
Affinity Capture-MS Homo sapiens
29 FLOT1 10211
Proximity Label-MS Homo sapiens
30 C3orf14  
Affinity Capture-MS Homo sapiens
31 ZNF263  
Affinity Capture-MS Homo sapiens
32 NDC80 10403
Proximity Label-MS Homo sapiens
33 EPHA2 1969
Proximity Label-MS Homo sapiens
34 NCS1 23413
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 YWHAQ 10971
Affinity Capture-MS Homo sapiens
36 STX7 8417
Proximity Label-MS Homo sapiens
37 KRAS 3845
Proximity Label-MS Homo sapiens
38 RHOB 388
Proximity Label-MS Homo sapiens
39 PCM1 5108
Proximity Label-MS Homo sapiens
40 YWHAG 7532
Affinity Capture-MS Homo sapiens
41 KCTD18  
Affinity Capture-MS Homo sapiens
42 LATS2 26524
Proximity Label-MS Homo sapiens
43 CTDSPL 10217
Proximity Label-MS Homo sapiens
44 MYH9 4627
Affinity Capture-MS Homo sapiens
45 C17orf59 54785
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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