Gene description for PTPRG
Gene name protein tyrosine phosphatase, receptor type, G
Gene symbol PTPRG
Other names/aliases HPTPG
PTPG
R-PTP-GAMMA
RPTPG
Species Homo sapiens
 Database cross references - PTPRG
ExoCarta ExoCarta_5793
Vesiclepedia VP_5793
Entrez Gene 5793
HGNC 9671
MIM 176886
UniProt P23470  
 PTPRG identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PTPRG
Molecular Function
    protein tyrosine phosphatase activity GO:0004725 IBA
    protein tyrosine phosphatase activity GO:0004725 IDA
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    transmembrane receptor protein tyrosine phosphatase activity GO:0005001 TAS
    protein binding GO:0005515 IPI
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    identical protein binding GO:0042802 IPI
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    signal transduction GO:0007165 IBA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 TAS
    negative regulation of epithelial cell migration GO:0010633 IMP
    negative regulation of neuron projection development GO:0010977 IEA
    neuron projection development GO:0031175 IBA
    regulation of homophilic cell adhesion GO:1903385 IMP
Subcellular Localization
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PTPRG in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTPRG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FCN1 2219
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PALM 5064
Proximity Label-MS Homo sapiens
3 LGALS9 3965
Affinity Capture-MS Homo sapiens
4 RAB35 11021
Proximity Label-MS Homo sapiens
5 PSD3 23362
Proximity Label-MS Homo sapiens
6 DUS4L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 FAM129B 64855
Proximity Label-MS Homo sapiens
8 CDC42BPB 9578
Proximity Label-MS Homo sapiens
9 NCSTN 23385
Affinity Capture-MS Homo sapiens
10 OCLN 100506658
Proximity Label-MS Homo sapiens
11 CEP89 84902
Proximity Label-MS Homo sapiens
12 MARCKS 4082
Proximity Label-MS Homo sapiens
13 LAMTOR1 55004
Proximity Label-MS Homo sapiens
14 DUSP22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SDC1 6382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LGALS1 3956
Affinity Capture-MS Homo sapiens
17 GJA1 2697
Proximity Label-MS Homo sapiens
18 PARD3 56288
Proximity Label-MS Homo sapiens
19 GPRIN1 114787
Proximity Label-MS Homo sapiens
20 LAMP1 3916
Proximity Label-MS Homo sapiens
21 IER3IP1 51124
Affinity Capture-MS Homo sapiens
22 ARMC12  
Affinity Capture-MS Homo sapiens
23 DUSP26 78986
Proximity Label-MS Homo sapiens
24 LAMP2 3920
Proximity Label-MS Homo sapiens
25 PCDHGC3 5098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PPIA 5478
Cross-Linking-MS (XL-MS) Homo sapiens
27 CDK2AP1  
Affinity Capture-MS Homo sapiens
28 ARF6 382
Proximity Label-MS Homo sapiens
29 DLG4  
Two-hybrid Homo sapiens
30 RPS15 6209
Proximity Label-MS Homo sapiens
31 CSF3 1440
Affinity Capture-MS Homo sapiens
32 C11orf52 91894
Proximity Label-MS Homo sapiens
33 CLNS1A 1207
Proximity Label-MS Homo sapiens
34 LMAN1 3998
Proximity Label-MS Homo sapiens
35 LYN 4067
Proximity Label-MS Homo sapiens
36 SEC24A 10802
Proximity Label-MS Homo sapiens
37 MCAM 4162
Proximity Label-MS Homo sapiens
38 DEFB104A  
Affinity Capture-MS Homo sapiens
39 HSPA12A 259217
Proximity Label-MS Homo sapiens
40 CERS2 29956
Affinity Capture-MS Homo sapiens
41 GJB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ADD2 119
Proximity Label-MS Homo sapiens
44 CCNL2 81669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 RAB2A 5862
Proximity Label-MS Homo sapiens
46 CLCC1 23155
Affinity Capture-MS Homo sapiens
47 FCHO2 115548
Proximity Label-MS Homo sapiens
48 DIRAS3  
Proximity Label-MS Homo sapiens
49 FBXO2 26232
Affinity Capture-MS Homo sapiens
50 SLC39A10 57181
Proximity Label-MS Homo sapiens
51 SCGB2A2  
Affinity Capture-MS Homo sapiens
52 ZNF517  
Affinity Capture-MS Homo sapiens
53 PTPRG 5793
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
54 PACS1 55690
Affinity Capture-MS Homo sapiens
55 PTPRB 5787
Proximity Label-MS Homo sapiens
56 CDV3 55573
Cross-Linking-MS (XL-MS) Homo sapiens
57 LAMP3  
Proximity Label-MS Homo sapiens
58 NF2 4771
Proximity Label-MS Homo sapiens
59 ACBD7  
Affinity Capture-MS Homo sapiens
60 ISG20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 KRAS 3845
Proximity Label-MS Homo sapiens
62 RHOB 388
Proximity Label-MS Homo sapiens
63 FYTTD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 LGALS3 3958
Affinity Capture-MS Homo sapiens
65 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 NPB  
Affinity Capture-MS Homo sapiens
67 PCGF3  
Affinity Capture-MS Homo sapiens
68 SERPINA12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 CAV1 857
Proximity Label-MS Homo sapiens
70 DUSP23 54935
Affinity Capture-MS Homo sapiens
71 CASKIN2  
Proximity Label-MS Homo sapiens
72 FARP1 10160
Proximity Label-MS Homo sapiens
73 TMEM185A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PTPRG is involved
No pathways found





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