Gene description for ADD2
Gene name adducin 2 (beta)
Gene symbol ADD2
Other names/aliases ADDB
Species Homo sapiens
 Database cross references - ADD2
ExoCarta ExoCarta_119
Vesiclepedia VP_119
Entrez Gene 119
HGNC 244
MIM 102681
UniProt P35612  
 ADD2 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ADD2
Molecular Function
    actin binding GO:0003779 IMP
    structural constituent of cytoskeleton GO:0005200 IEA
    calmodulin binding GO:0005516 IEA
    protein kinase binding GO:0019901 IPI
    spectrin binding GO:0030507 IDA
    protein homodimerization activity GO:0042803 IPI
    protein heterodimerization activity GO:0046982 IPI
    protein dimerization activity GO:0046983 IPI
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
Biological Process
    synapse assembly GO:0007416 IEA
    actin cytoskeleton organization GO:0030036 IC
    hemopoiesis GO:0030097 IEA
    positive regulation of protein binding GO:0032092 IDA
    leukocyte migration GO:0050900 IMP
    leukocyte tethering or rolling GO:0050901 IMP
    barbed-end actin filament capping GO:0051016 IBA
    barbed-end actin filament capping GO:0051016 IDA
    actin filament bundle assembly GO:0051017 IDA
    protein-containing complex assembly GO:0065003 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    plasma membrane GO:0005886 IBA
    F-actin capping protein complex GO:0008290 IDA
    postsynaptic density GO:0014069 IBA
    cytoplasmic vesicle GO:0031410 IDA
    plasma membrane raft GO:0044853 IMP
 Experiment description of studies that identified ADD2 in sEVs
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 INTS4  
Affinity Capture-MS Homo sapiens
4 ADD1 118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PTPRG 5793
Proximity Label-MS Homo sapiens
6 MECP2 4204
Affinity Capture-MS Homo sapiens
7 RAB35 11021
Proximity Label-MS Homo sapiens
8 NUDT12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 FEM1A  
Affinity Capture-MS Homo sapiens
10 ROCK1 6093
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 OCLN 100506658
Proximity Label-MS Homo sapiens
12 DEF6 50619
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ABRA  
Affinity Capture-MS Homo sapiens
14 MARCKS 4082
Proximity Label-MS Homo sapiens
15 MME 4311
Affinity Capture-MS Homo sapiens
16 LAMP1 3916
Proximity Label-MS Homo sapiens
17 SERPINB13 5275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ALDH16A1 126133
Affinity Capture-MS Homo sapiens
19 ADD3 120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ANKRD29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ROCK2 9475
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CTAG2  
Affinity Capture-MS Homo sapiens
23 CXADR 1525
Proximity Label-MS Homo sapiens
24 C11orf52 91894
Proximity Label-MS Homo sapiens
25 PNKD  
Affinity Capture-MS Homo sapiens
26 APP 351
Reconstituted Complex Homo sapiens
27 LYN 4067
Proximity Label-MS Homo sapiens
28 CTDSPL 10217
Proximity Label-MS Homo sapiens
29 TCIRG1 10312
Affinity Capture-MS Homo sapiens
30 DPPA4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 DNAJC5 80331
Proximity Label-MS Homo sapiens
32 LBH  
Affinity Capture-MS Homo sapiens
33 FYN 2534
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
34 RPA3 6119
Proximity Label-MS Homo sapiens
35 TRIM21 6737
Affinity Capture-MS Homo sapiens
36 KIF23 9493
Affinity Capture-MS Homo sapiens
37 SALL1 6299
Affinity Capture-MS Homo sapiens
38 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 EPHA2 1969
Proximity Label-MS Homo sapiens
40 ZNF641  
Affinity Capture-MS Homo sapiens
41 STX7 8417
Proximity Label-MS Homo sapiens
42 ACTB 60
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 KRAS 3845
Proximity Label-MS Homo sapiens
44 RPS14P3  
Affinity Capture-MS Homo sapiens
45 CRISP3  
Affinity Capture-MS Homo sapiens
46 CAV1 857
Proximity Label-MS Homo sapiens
47 DUSP23 54935
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PECAM1 5175
Affinity Capture-MS Homo sapiens
49 GRAMD1B  
Affinity Capture-MS Homo sapiens
50 FLOT1 10211
Proximity Label-MS Homo sapiens
51 RPH3A 22895
Reconstituted Complex Homo sapiens
52 ESCO2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ADD2 is involved
PathwayEvidenceSource
Miscellaneous transport and binding events TAS Reactome
Transport of small molecules TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here