Gene description for ACTB
Gene name actin, beta
Gene symbol ACTB
Other names/aliases BRWS1
PS1TP5BP1
Species Homo sapiens
 Database cross references - ACTB
ExoCarta ExoCarta_60
Vesiclepedia VP_60
Entrez Gene 60
HGNC 132
MIM 102630
UniProt P60709  
 ACTB identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 16302729    
Breast cancer cells 19415654    
Breast cancer cells 33738083    
Breast cancer cells 33738083    
Breast cancer cells 34108659    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast milk 17641064    
Breast milk 17641064    
Cervical cancer cells 33767144    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Colorectal cancer cells 22740476    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25907253    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Faeces 33431899    
Faeces 33431899    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 36581340    
Gastric cancer cells 36581340    
Gastric cancer cells 36581340    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Intestinal epithelial cells 11487543    
Keratinocytes 19530224    
Keratinocytes 19530224    
Lung cancer cells 32132711    
Lung cancer cells 33589572    
Lung cancer cells 33589572    
Lymphoma cells 34108659    
Malignant pleural effusions 14975938    
Malignant pleural effusions 14975938    
Melanoma cells 15478216    
Melanoma cells 22635005    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 34957415    
Melanoma cells 34957415    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Mesothelioma cells 15111327    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic duct epithalial cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 18520029    
Saliva 19199708    
Tracheobronchial cells 19190083    
Urine 15326289    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ACTB
Molecular Function
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IDA
    kinesin binding GO:0019894 IPI
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IPI
    Tat protein binding GO:0030957 IPI
    nucleosomal DNA binding GO:0031492 HDA
    identical protein binding GO:0042802 IPI
    tau protein binding GO:0048156 NAS
    nitric-oxide synthase binding GO:0050998 IPI
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 EXP
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 IBA
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 IDA
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 IMP
Biological Process
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 ISS
    morphogenesis of a polarized epithelium GO:0001738 IMP
    chromatin remodeling GO:0006338 HDA
    chromatin remodeling GO:0006338 NAS
    regulation of transcription by RNA polymerase II GO:0006357 NAS
    establishment or maintenance of cell polarity GO:0007163 IMP
    axonogenesis GO:0007409 IBA
    positive regulation of cell population proliferation GO:0008284 NAS
    substantia nigra development GO:0021762 HEP
    regulation of transmembrane transporter activity GO:0022898 IGI
    regulation of transmembrane transporter activity GO:0022898 ISS
    regulation of mitotic metaphase/anaphase transition GO:0030071 NAS
    adherens junction assembly GO:0034333 IMP
    maintenance of blood-brain barrier GO:0035633 NAS
    regulation of apoptotic process GO:0042981 NAS
    apical protein localization GO:0045176 IMP
    positive regulation of T cell differentiation GO:0045582 NAS
    negative regulation of cell differentiation GO:0045596 NAS
    positive regulation of cell differentiation GO:0045597 NAS
    positive regulation of myoblast differentiation GO:0045663 NAS
    positive regulation of DNA-templated transcription GO:0045893 NAS
    cell motility GO:0048870 IBA
    cell motility GO:0048870 IMP
    regulation of norepinephrine uptake GO:0051621 IGI
    regulation of norepinephrine uptake GO:0051621 ISS
    positive regulation of norepinephrine uptake GO:0051623 TAS
    regulation of cell cycle GO:0051726 IMP
    regulation of G0 to G1 transition GO:0070316 NAS
    platelet aggregation GO:0070527 HMP
    protein localization to adherens junction GO:0071896 IMP
    cellular response to cytochalasin B GO:0072749 IMP
    postsynaptic actin cytoskeleton organization GO:0098974 IEA
    regulation of transepithelial transport GO:0150111 IMP
    regulation of synaptic vesicle endocytosis GO:1900242 IEA
    positive regulation of stem cell population maintenance GO:1902459 NAS
    regulation of protein localization to plasma membrane GO:1903076 IMP
    positive regulation of double-strand break repair via homologous recombination GO:1905168 IDA
    regulation of G1/S transition of mitotic cell cycle GO:2000045 NAS
    regulation of double-strand break repair GO:2000779 NAS
    positive regulation of double-strand break repair GO:2000781 NAS
    regulation of nucleotide-excision repair GO:2000819 NAS
Subcellular Localization
    kinetochore GO:0000776 NAS
    chromatin GO:0000785 HDA
    chromatin GO:0000785 NAS
    nucleosome GO:0000786 IDA
    extracellular space GO:0005615 HDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IDA
    cytoskeleton GO:0005856 ISS
    cytoskeleton GO:0005856 TAS
    actin filament GO:0005884 IBA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    brush border GO:0005903 IEA
    cell-cell junction GO:0005911 IMP
    adherens junction GO:0005912 IDA
    focal adhesion GO:0005925 HDA
    actin cytoskeleton GO:0015629 IBA
    actin cytoskeleton GO:0015629 IDA
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    nuclear matrix GO:0016363 NAS
    SWI/SNF complex GO:0016514 NAS
    RSC-type complex GO:0016586 NAS
    lamellipodium GO:0030027 IDA
    axon GO:0030424 IBA
    cortical cytoskeleton GO:0030863 IEA
    vesicle GO:0031982 HDA
    protein-containing complex GO:0032991 HDA
    protein-containing complex GO:0032991 IDA
    brahma complex GO:0035060 NAS
    NuA4 histone acetyltransferase complex GO:0035267 IBA
    NuA4 histone acetyltransferase complex GO:0035267 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
    apical junction complex GO:0043296 IDA
    calyx of Held GO:0044305 IEA
    synapse GO:0045202 IBA
    extracellular exosome GO:0070062 HDA
    tight junction GO:0070160 IDA
    npBAF complex GO:0071564 NAS
    nBAF complex GO:0071565 NAS
    blood microparticle GO:0072562 HDA
    dense body GO:0097433 ISS
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    presynapse GO:0098793 TAS
    postsynaptic actin cytoskeleton GO:0098871 IDA
    postsynaptic actin cytoskeleton GO:0098871 IMP
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    bBAF complex GO:0140092 NAS
    GBAF complex GO:0140288 NAS
    ribonucleoprotein complex GO:1990904 IDA
 Experiment description of studies that identified ACTB in exosomes
1
Experiment ID 79
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 76
MISEV standards
EM
Biophysical techniques
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
5
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 19
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16302729    
Organism Homo sapiens
Experiment description "Purification, characterization and biological significance of tumor-derived exosomes."
Authors "Koga K, Matsumoto K, Akiyoshi T, Kubo M, Yamanaka N, Tasaki A, Nakashima H, Nakamura M, Kuroki S, Tanaka M, Katano M"
Journal name ACR
Publication year 2005
Sample Breast cancer cells
Sample name BT-474
MDA-MB-231
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Filtration
Immunobeads(HER2)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
10
Experiment ID 65
MISEV standards
Biophysical techniques
HSP60|HSP27|GAPDH|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 19415654    
Organism Homo sapiens
Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7.
Authors "Staubach S, Razawi H, Hanisch FG."
Journal name PROTEOMICS
Publication year 2009
Sample Breast cancer cells
Sample name MCF-7
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
11
Experiment ID 397
MISEV standards
EM
Biophysical techniques
CD9|TSG101|CD63
Enriched markers
GOLGA2|CANX
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33738083    
Organism Homo sapiens
Experiment description Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases
Authors "Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH"
Journal name J Extracell Vesicles
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunofluorescence
Flow cytomtery
12
Experiment ID 398
MISEV standards
EM
Biophysical techniques
CD9|TSG101|CD63
Enriched markers
GOLGA2|CANX
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33738083    
Organism Homo sapiens
Experiment description Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases
Authors "Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH"
Journal name J Extracell Vesicles
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunofluorescence
Flow cytomtery
13
Experiment ID 412
MISEV standards
EM
Biophysical techniques
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
CANX|ACTB
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
14
Experiment ID 1181
MISEV standards
EM
Biophysical techniques
TSG101|CD81|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 1182
MISEV standards
EM
Biophysical techniques
TSG101|CD81|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 1183
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 1
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density 1.05 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
17
Experiment ID 1184
MISEV standards
Biophysical techniques
TSG101|FLOT1|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 5
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
18
Experiment ID 1185
MISEV standards
Biophysical techniques
TSG101|FLOT1|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
19
Experiment ID 1186
MISEV standards
Biophysical techniques
TSG101|FLOT1|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 7
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
20
Experiment ID 1187
MISEV standards
Biophysical techniques
FLOT1|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 8
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 1188
MISEV standards
Biophysical techniques
FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 9
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 1192
MISEV standards
Biophysical techniques
FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 5
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
23
Experiment ID 1193
MISEV standards
Biophysical techniques
TSG101|FLOT1|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
24
Experiment ID 1194
MISEV standards
Biophysical techniques
FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 7
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
25
Experiment ID 1195
MISEV standards
Biophysical techniques
FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 8
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
26
Experiment ID 1196
MISEV standards
Biophysical techniques
FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 9
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
27
Experiment ID 1197
MISEV standards
Biophysical techniques
TSG101|CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
28
Experiment ID 1198
MISEV standards
Biophysical techniques
TSG101|CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
29
Experiment ID 46
MISEV standards
EM|IEM
Biophysical techniques
HSC70|CD63|MHCII|CD81|CD86
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
30
Experiment ID 48
MISEV standards
EM|IEM
Biophysical techniques
HSC70|CD63|CD86|MHCII
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
31
Experiment ID 388
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33767144    
Organism Homo sapiens
Experiment description Quantitative characterization of extracellular vesicle uptake and content delivery within mammalian cells
Authors "Bonsergent E, Grisard E, Buchrieser J, Schwartz O, Théry C, Lavieu G."
Journal name Nat Commun
Publication year 2021
Sample Cervical cancer cells
Sample name HeLa
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
32
Experiment ID 494
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
NTA
Particle analysis