Gene description for Hspa1b
Gene name heat shock protein 1B
Gene symbol Hspa1b
Other names/aliases Hsp70
Hsp70-1
Hsp70.1
hsp68
Species Mus musculus
 Database cross references - Hspa1b
ExoCarta ExoCarta_15511
Entrez Gene 15511
UniProt P17879  
 Hspa1b identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Neural stem cells 25242146    
Oligodendrocytes 21136642    
 Gene ontology annotations for Hspa1b
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of apoptotic process GO:0043066 IDA
    response to heat GO:0009408 IDA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IDA
    binding of sperm to zona pellucida GO:0007339 IDA
Subcellular Localization
    intracellular GO:0005622 IDA
    mitochondrial matrix GO:0005759 ISO
    mitochondrion GO:0005739 ISS
    cell body GO:0044297 IDA
    zona pellucida receptor complex GO:0002199 IDA
 Experiment description of studies that identified Hspa1b in exosomes
1
Experiment ID 73
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 262
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|HSP90
EV Cytosolic markers
✔
CD63|CD9|
EV Membrane markers
✘ EV Negative markers
✔
Y
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
3
Experiment ID 33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Hspa1b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTB 60
Reconstituted Complex Homo sapiens
2 Hsp90ab1 15516
Reconstituted Complex Mus musculus
View the network image/svg+xml



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