Gene description for Bag5
Gene name BCL2-associated athanogene 5
Gene symbol Bag5
Other names/aliases 1600025G07Rik
4930405J06Rik
Species Mus musculus
 Database cross references - Bag5
ExoCarta ExoCarta_70369
Vesiclepedia VP_70369
Entrez Gene 70369
UniProt Q8CI32  
 Bag5 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Bag5
Molecular Function
    protein kinase binding GO:0019901 ISO
    chaperone binding GO:0051087 ISS
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 ISO
Biological Process
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 ISO
    negative regulation of protein refolding GO:0061084 ISS
    neuron death GO:0070997 ISS
    regulation of inclusion body assembly GO:0090083 ISS
    negative regulation of ubiquitin-protein transferase activity GO:0051444 ISS
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISO
    negative regulation of protein ubiquitination GO:0031397 ISS
Subcellular Localization
    membrane GO:0016020 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    inclusion body GO:0016234 ISS
    nucleus GO:0005634 ISO
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Bag5 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Bag5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Bag5 is involved
PathwayEvidenceSource
Regulation of HSF1-mediated heat shock response IEA Reactome





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