Gene description for Pfn1
Gene name profilin 1
Gene symbol Pfn1
Other names/aliases Pfn
Species Mus musculus
 Database cross references - Pfn1
ExoCarta ExoCarta_18643
Vesiclepedia VP_18643
Entrez Gene 18643
UniProt P62962  
 Pfn1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Dendritic cells 11390481    
Mast cells 17486113    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pfn1
Molecular Function
    adenyl-nucleotide exchange factor activity GO:0000774 IEA
    adenyl-nucleotide exchange factor activity GO:0000774 ISO
    phosphotyrosine residue binding GO:0001784 IEA
    phosphotyrosine residue binding GO:0001784 ISO
    actin binding GO:0003779 IBA
    actin binding GO:0003779 ISO
    actin monomer binding GO:0003785 IEA
    actin monomer binding GO:0003785 ISO
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IEA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISO
    small GTPase binding GO:0031267 IPI
    proline-rich region binding GO:0070064 IEA
    proline-rich region binding GO:0070064 ISO
Biological Process
    neural tube closure GO:0001843 IGI
    regulation of transcription by RNA polymerase II GO:0006357 IGI
    regulation of actin polymerization or depolymerization GO:0008064 TAS
    positive regulation of epithelial cell migration GO:0010634 IEA
    positive regulation of epithelial cell migration GO:0010634 ISO
    actin cytoskeleton organization GO:0030036 IEA
    regulation of actin filament polymerization GO:0030833 IBA
    negative regulation of actin filament polymerization GO:0030837 ISO
    positive regulation of actin filament polymerization GO:0030838 IEA
    positive regulation of actin filament polymerization GO:0030838 ISO
    negative regulation of actin filament bundle assembly GO:0032232 ISO
    positive regulation of actin filament bundle assembly GO:0032233 IBA
    positive regulation of ATP-dependent activity GO:0032781 ISO
    sequestering of actin monomers GO:0042989 TAS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of viral transcription GO:0050434 ISO
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    positive regulation of DNA metabolic process GO:0051054 ISO
    positive regulation of stress fiber assembly GO:0051496 ISO
    negative regulation of stress fiber assembly GO:0051497 IEA
    negative regulation of stress fiber assembly GO:0051497 ISO
    synapse maturation GO:0060074 IDA
    synapse maturation GO:0060074 IMP
    modification of postsynaptic actin cytoskeleton GO:0098885 IDA
    modification of postsynaptic actin cytoskeleton GO:0098885 IMP
    positive regulation of ruffle assembly GO:1900029 IEA
    positive regulation of ruffle assembly GO:1900029 ISO
Subcellular Localization
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    cell cortex GO:0005938 IEA
    cell cortex GO:0005938 ISO
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    parallel fiber to Purkinje cell synapse GO:0098688 ISO
    presynapse GO:0098793 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Pfn1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pfn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3b2  
Two-hybrid Mus musculus
2 Pfn2  
Affinity Capture-Western Mus musculus
3 Tubb5 22154
Affinity Capture-MS Mus musculus
4 Glrb  
Affinity Capture-Western Rattus norvegicus
5 DMRTB1  
Two-hybrid Homo sapiens
6 Dnm1 13429
Affinity Capture-Western Mus musculus
7 DDX17 10521
Two-hybrid Homo sapiens
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Tfe3  
Affinity Capture-MS Mus musculus
10 Atg16l1  
Affinity Capture-MS Mus musculus
11 Actb 11461
Two-hybrid Mus musculus
Affinity Capture-MS Mus musculus
12 Taf15  
Two-hybrid Mus musculus
13 Dcaf13 223499
Two-hybrid Mus musculus
14 Tmem173  
Proximity Label-MS Mus musculus
15 Mypop  
Affinity Capture-Western Mus musculus
16 USO1 8615
Two-hybrid Homo sapiens
17 Hspa1b 15511
Affinity Capture-MS Mus musculus
18 CPSF7 79869
Two-hybrid Homo sapiens
19 Eed  
Affinity Capture-MS Mus musculus
20 Pclo  
Affinity Capture-Western Mus musculus
21 Cltc 67300
Affinity Capture-MS Mus musculus
22 Vcp 269523
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
23 Cpsf6  
Two-hybrid Mus musculus
24 BAG4  
Two-hybrid Homo sapiens
25 Fancd2  
Affinity Capture-MS Mus musculus
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