Gene description for Pfn1
Gene name profilin 1
Gene symbol Pfn1
Other names/aliases Pfn
Species Mus musculus
 Database cross references - Pfn1
ExoCarta ExoCarta_18643
Entrez Gene 18643
UniProt P62962  
 Pfn1 identified in exosomes derived from the following tissue/cell type
Dendritic cells 11390481    
Mast cells 17486113    
Mov neuroglial cells 15210972    
 Gene ontology annotations for Pfn1
Molecular Function
    receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    actin monomer binding GO:0003785 ISO
    poly(A) RNA binding GO:0044822 ISO
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISO
    Rho GTPase binding GO:0017048 IPI
    adenyl-nucleotide exchange factor activity GO:0000774 ISO
    proline-rich region binding GO:0070064 ISO
    actin binding GO:0003779 ISO
Biological Process
    sequestering of actin monomers GO:0042989 TAS
    positive regulation of stress fiber assembly GO:0051496 ISO
    protein stabilization GO:0050821 ISO
    positive regulation of actin filament polymerization GO:0030838 ISO
    negative regulation of actin filament polymerization GO:0030837 ISO
    negative regulation of stress fiber assembly GO:0051497 ISO
    positive regulation of DNA metabolic process GO:0051054 ISO
    negative regulation of actin filament bundle assembly GO:0032232 ISO
    positive regulation of viral transcription GO:0050434 ISO
    positive regulation of ATPase activity GO:0032781 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    positive regulation of ruffle assembly GO:1900029 ISO
    regulation of transcription from RNA polymerase II promoter GO:0006357 IGI
    regulation of actin polymerization or depolymerization GO:0008064 TAS
    positive regulation of epithelial cell migration GO:0010634 ISO
    neural tube closure GO:0001843 IGI
    actin cytoskeleton organization GO:0030036 IEA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    neuron projection GO:0043005 ISO
    nucleus GO:0005634 ISO
    blood microparticle GO:0072562 ISO
    membrane GO:0016020 ISO
    synapse GO:0045202 ISO
    focal adhesion GO:0005925 ISO
    cytoskeleton GO:0005856 IEA
    cytoplasm GO:0005737 ISO
    cell GO:0005623 TAS
    cell cortex GO:0005938 ISO
 Experiment description of studies that identified Pfn1 in exosomes
1
Experiment ID 10
ISEV standards
EM
EV Biophysical techniques
Alix
EV Cytosolic markers
CD9|MHCII|MHCI|CD86|LAMP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S.
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 39
ISEV standards
IEM
EV Biophysical techniques
TSG101|HSC70
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Pfn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pfn1 is involved
PathwayEvidenceSource
PCP/CE pathway IEA Reactome
Platelet degranulation IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Signaling by Robo receptor IEA Reactome





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