Experiment description of studies that identified Pfn1 in exosomes |
1 |
Experiment ID |
10 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix
|
EV Cytosolic markers |
✔
CD9|MHCII|MHCI|CD86|LAMP2
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [MALDI TOF]
|
PubMed ID |
11390481
|
Organism |
Mus musculus |
Experiment description |
Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles. |
Authors |
Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S. |
Journal name |
JIMMU
|
Publication year |
2001 |
Sample |
Dendritic cells |
Sample name |
D1 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.15-1.18 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] Western blotting |
|
|
2 |
Experiment ID |
15 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
17486113
|
Organism |
Mus musculus Homo sapiens |
Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
Authors |
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO |
Journal name |
NCB
|
Publication year |
2007 |
Sample |
Mast cells |
Sample name |
MC9 Bone marrow-derived mast cells HMC-1 |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.11-1.21 g/mL
|
Molecules identified in the study |
Protein mRNA miRNA |
Methods used in the study |
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array |
|
|
3 |
Experiment ID |
39 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
TSG101|HSC70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
15210972
|
Organism |
Mus musculus |
Experiment description |
Cells release prions in association with exosomes. |
Authors |
Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G |
Journal name |
PNAS
|
Publication year |
2004 |
Sample |
Mov neuroglial cells |
Sample name |
Mov neuroglial cell |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.14 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy |
|
|