Gene description for Vcp
Gene name valosin containing protein
Gene symbol Vcp
Other names/aliases 3110001E05
CDC48
p97
p97/VCP
Species Mus musculus
 Database cross references - Vcp
ExoCarta ExoCarta_269523
Entrez Gene 269523
UniProt Q01853  
 Vcp identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Oligodendrocytes 21136642    
 Gene ontology annotations for Vcp
Molecular Function
    ATPase activity GO:0016887 ISO
    identical protein binding GO:0042802 ISO
    poly(A) RNA binding GO:0044822 ISO
    protein domain specific binding GO:0019904 ISO
    deubiquitinase activator activity GO:0035800 ISO
    receptor binding GO:0005102 ISO
    ubiquitin-specific protease binding GO:1990381 ISO
    polyubiquitin binding GO:0031593 ISO
    ubiquitin-like protein ligase binding GO:0044389 ISO
    hydrolase activity GO:0016787 IEA
    ADP binding GO:0043531 ISO
    lipid binding GO:0008289 IEA
    protein binding GO:0005515 IPI
    protein phosphatase binding GO:0019903 ISO
    protein complex binding GO:0032403 ISO
    ATP binding GO:0005524 ISO
    nucleotide binding GO:0000166 IEA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IGI
    aggresome assembly GO:0070842 IGI
    positive regulation of protein K63-linked deubiquitination GO:1903006 ISO
    ER-associated misfolded protein catabolic process GO:0071712 ISO
    protein N-linked glycosylation via asparagine GO:0018279 ISO
    DNA repair GO:0006281 IEA
    positive regulation of protein complex assembly GO:0031334 ISO
    cellular response to DNA damage stimulus GO:0006974 ISO
    translesion synthesis GO:0019985 ISO
    transport GO:0006810 IEA
    double-strand break repair GO:0006302 ISO
    viral genome replication GO:0019079 ISO
    ER-associated ubiquitin-dependent protein catabolic process GO:0030433 ISO
    protein hexamerization GO:0034214 ISO
    protein ubiquitination GO:0016567 ISO
    positive regulation of protein catabolic process GO:0045732 ISO
    positive regulation of Lys63-specific deubiquitinase activity GO:1903007 ISO
    protein homooligomerization GO:0051260 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    ER to Golgi vesicle-mediated transport GO:0006888 ISO
    retrograde protein transport, ER to cytosol GO:0030970 ISO
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 IDA
Subcellular Localization
    VCP-NPL4-UFD1 AAA ATPase complex GO:0034098 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    lipid particle GO:0005811 ISO
    myelin sheath GO:0043209 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    proteasome complex GO:0000502 ISO
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
    site of double-strand break GO:0035861 ISO
    protein complex GO:0043234 IPI
    Hrd1p ubiquitin ligase complex GO:0000836 ISO
    endoplasmic reticulum GO:0005783 ISO
    VCP-NSFL1C complex GO:1990730 ISO
    nucleoplasm GO:0005654 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Vcp in exosomes
1
Experiment ID 26
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|GAPDH|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Vcp
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Vcp is involved
PathwayEvidenceSource
Hedgehog ligand biogenesis IEA Reactome
HSF1 activation IEA Reactome
Translesion Synthesis by POLH IEA Reactome





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