Gene ontology annotations for Hspa8 |
|
Experiment description of studies that identified Hspa8 in exosomes |
1 |
Experiment ID |
8 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
HSP60|HSC70
|
EV Cytosolic markers |
✔
MHCII|CD80|CD86
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
12626558
|
Organism |
Mus musculus |
Experiment description |
Mast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo. |
Authors |
Skokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S. |
Journal name |
JIMMU
|
Publication year |
2003 |
Sample |
B cells |
Sample name |
B cells |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
2 |
Experiment ID |
89 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20424270
|
Organism |
Rattus norvegicus |
Experiment description |
Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins. |
Authors |
Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M. |
Journal name |
JLR
|
Publication year |
2010 |
Sample |
Basophilic leukemia cells |
Sample name |
RBL-2H3 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Western blotting Gas chromatography Thin layer chromatography Mass spectrometry [Qstar XL] FACS |
|
|
3 |
Experiment ID |
89 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20424270
|
Organism |
Rattus norvegicus |
Experiment description |
Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins. |
Authors |
Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M. |
Journal name |
JLR
|
Publication year |
2010 |
Sample |
Basophilic leukemia cells |
Sample name |
RBL-2H3 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Western blotting Gas chromatography Thin layer chromatography Mass spectrometry [Qstar XL] FACS |
|
|
4 |
Experiment ID |
26 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|GAPDH|HSP70
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19109410
|
Organism |
Mus musculus |
Experiment description |
Proteomic and immunologic analyses of brain tumor exosomes. |
Authors |
Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD |
Journal name |
FASEB
|
Publication year |
2008 |
Sample |
Brain cancer cells |
Sample name |
SMA560vIII |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.13-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF/TOF] Western blotting |
|
|
5 |
Experiment ID |
3 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
HSP84|HSC73
|
EV Cytosolic markers |
✔
MHCII|CD9|mac-1
|
EV Membrane markers |
✔
CANX|HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry Western blotting
|
PubMed ID |
10545503
|
Organism |
Mus musculus |
Experiment description |
Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73. |
Authors |
Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S. |
Journal name |
JCB
|
Publication year |
1999 |
Sample |
Dendritic cells |
Sample name |
D1 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.14-1.20 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF] Western blotting |
|
|
6 |
Experiment ID |
73 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
18494037
|
Organism |
Mus musculus |
Experiment description |
Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes. |
Authors |
Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ |
Journal name |
ELEC
|
Publication year |
2008 |
Sample |
Embryonic fibroblasts |
Sample name |
NIH3T3 |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LCQ DECA] Western blotting |
|
|
7 |
Experiment ID |
210 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD81|FLOT1
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23260141
|
Organism |
Mus musculus |
Experiment description |
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. |
Authors |
Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL. |
Journal name |
Cell
|
Publication year |
2012 |
Sample |
Fibroblasts |
Sample name |
Normal-Fibroblasts (L cells) |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
8 |
Experiment ID |
9 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
HSP60|HSC70
|
EV Cytosolic markers |
✔
MHCII|CD80|CD87
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
12626558
|
Organism |
Mus musculus |
Experiment description |
Mast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo. |
Authors |
Skokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S. |
Journal name |
JIMMU
|
Publication year |
2003 |
Sample |
Macrophages |
Sample name |
Macrophages |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
9 |
Experiment ID |
214 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
Leishmania-infected-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
10 |
Experiment ID |
215 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
LPS-treated-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
11 |
Experiment ID |
216 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
Normal-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
12 |
Experiment ID |
7 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
HSP60|HSC70
|
EV Cytosolic markers |
✔
MHCII|CD80|CD86
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
12626558
|
Organism |
Mus musculus |
Experiment description |
Mast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo. |
Authors |
Skokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S. |
Journal name |
JIMMU
|
Publication year |
2003 |
Sample |
Mast cells |
Sample name |
Bone marrow-derived mast cells |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
13 |
Experiment ID |
15 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
17486113
|
Organism |
Mus musculus Homo sapiens |
Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
Authors |
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO |
Journal name |
NCB
|
Publication year |
2007 |
Sample |
Mast cells |
Sample name |
MC9 Bone marrow-derived mast cells HMC-1 |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.11-1.21 g/mL
|
Molecules identified in the study |
Protein mRNA miRNA |
Methods used in the study |
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array |
|
|
14 |
Experiment ID |
14 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
RAB7|RAB11
|
EV Cytosolic markers |
✔
CD9|CD63|LAMP1|LAMP2
|
EV Membrane markers |
✔
DNM
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
16081791
|
Organism |
Mus musculus |
Experiment description |
Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. |
Authors |
Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L |
Journal name |
JIMMU
|
Publication year |
2005 |
Sample |
Microglia |
Sample name |
N9 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LCQ DECA XP] Western blotting |
|
|
15 |
Experiment ID |
39 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
TSG101|HSC70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry Western blotting
|
PubMed ID |
15210972
|
Organism |
Mus musculus |
Experiment description |
Cells release prions in association with exosomes. |
Authors |
Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G |
Journal name |
PNAS
|
Publication year |
2004 |
Sample |
Mov neuroglial cells |
Sample name |
Mov neuroglial cell |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.14 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy |
|
|
16 |
Experiment ID |
33 |
ISEV standards |
✔
EM|IEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21136642
|
Organism |
Mus musculus |
Experiment description |
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? |
Authors |
Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J |
Journal name |
PROTEOMICS_CL
|
Publication year |
2007 |
Sample |
Oligodendrocytes |
Sample name |
Oligodendrocytes Oli-neu |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.10-1.14 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy |
|
|
17 |
Experiment ID |
188 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
GAPDH|UCHL1|HSP90
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19351151
|
Organism |
Mus musculus |
Experiment description |
Characterization of vesicles secreted from insulinoma NIT-1 cells. |
Authors |
Lee HS, Jeong J, Lee KJ. |
Journal name |
J Proteome Res
|
Publication year |
2009 |
Sample |
Pancreatic cells |
Sample name |
Pancreatic beta cell (NIT-1) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
Protein-protein interactions for Hspa8 |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
No interactions are found.
|
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Pathways in which Hspa8 is involved |
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