Gene description for Stip1
Gene name stress-induced phosphoprotein 1
Gene symbol Stip1
Other names/aliases Hop
Sti1
p60
Species Mus musculus
 Database cross references - Stip1
ExoCarta ExoCarta_20867
Entrez Gene 20867
UniProt Q60864  
 Stip1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Stip1
Molecular Function
    protein C-terminus binding GO:0008022 ISO
    Hsp70 protein binding GO:0030544 ISO
    protein binding GO:0005515 IPI
    chaperone binding GO:0051087 ISO
    poly(A) RNA binding GO:0044822 ISO
Subcellular Localization
    myelin sheath GO:0043209 IDA
    cytoplasm GO:0005737 IEA
    nucleus GO:0005634 IEA
    protein complex GO:0043234 ISO
 Experiment description of studies that identified Stip1 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Stip1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Stip1 is involved
PathwayEvidenceSource
CHL1 interactions IEA Reactome





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