Gene description for Fkbp4
Gene name FK506 binding protein 4
Gene symbol Fkbp4
Other names/aliases 59kDa
AL022792
AW208983
FKBP-4
FKBP-52
FKBP52
FKPB52
p59
Species Mus musculus
 Database cross references - Fkbp4
ExoCarta ExoCarta_14228
Vesiclepedia VP_14228
Entrez Gene 14228
UniProt P30416  
 Fkbp4 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Fkbp4
Molecular Function
    phosphoprotein binding GO:0051219 IDA
    ATP binding GO:0005524 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 ISO
    copper-dependent protein binding GO:0032767 ISO
    heat shock protein binding GO:0031072 ISO
    tau protein binding GO:0048156 ISO
    FK506 binding GO:0005528 IBA
    poly(A) RNA binding GO:0044822 ISO
    isomerase activity GO:0016853 IEA
    glucocorticoid receptor binding GO:0035259 IDA
Biological Process
    copper ion transport GO:0006825 ISO
    reproductive structure development GO:0048608 IMP
    steroid hormone receptor complex assembly GO:0006463 IDA
    protein peptidyl-prolyl isomerization GO:0000413 ISO
    protein folding GO:0006457 IEA
    embryo implantation GO:0007566 IMP
    prostate gland development GO:0030850 IMP
    protein complex localization GO:0031503 IDA
    male sex differentiation GO:0046661 IMP
    androgen receptor signaling pathway GO:0030521 IMP
    negative regulation of microtubule polymerization GO:0031115 ISO
    chaperone-mediated protein folding GO:0061077 ISO
Subcellular Localization
    neuron projection GO:0043005 ISO
    nucleus GO:0005634 IDA
    axonal growth cone GO:0044295 ISO
    cytoskeleton GO:0005856 IEA
    cytosol GO:0005829 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    protein complex GO:0043234 ISO
    cytoplasm GO:0005737 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    nucleoplasm GO:0005654 ISO
    microtubule GO:0005874 IEA
    neuronal cell body GO:0043025 ISO
    mitochondrion GO:0005739 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Fkbp4 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Fkbp4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACVR1 90
Affinity Capture-Luminescence Homo sapiens
2 SMURF2 64750
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml
 Pathways in which Fkbp4 is involved
PathwayEvidenceSource
Attenuation phase IEA Reactome





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