Gene description for Fkbp4
Gene name FK506 binding protein 4
Gene symbol Fkbp4
Other names/aliases 59kDa
AL022792
AW208983
FKBP-4
FKBP-52
FKBP52
FKPB52
p59
Species Mus musculus
 Database cross references - Fkbp4
ExoCarta ExoCarta_14228
Vesiclepedia VP_14228
Entrez Gene 14228
UniProt P30416  
 Fkbp4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Fkbp4
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IBA
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 ISO
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    GTP binding GO:0005525 IDA
    heat shock protein binding GO:0031072 IDA
    heat shock protein binding GO:0031072 ISO
    copper-dependent protein binding GO:0032767 IEA
    copper-dependent protein binding GO:0032767 ISO
    nuclear glucocorticoid receptor binding GO:0035259 IDA
    tau protein binding GO:0048156 IEA
    tau protein binding GO:0048156 ISO
    phosphoprotein binding GO:0051219 IDA
    RNA polymerase II CTD heptapeptide repeat P3 isomerase activity GO:0140839 IEA
    RNA polymerase II CTD heptapeptide repeat P6 isomerase activity GO:0140840 IEA
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    steroid hormone receptor complex assembly GO:0006463 IDA
    copper ion transport GO:0006825 IEA
    copper ion transport GO:0006825 ISO
    embryo implantation GO:0007566 IMP
    negative regulation of neuron projection development GO:0010977 ISS
    androgen receptor signaling pathway GO:0030521 IMP
    prostate gland development GO:0030850 IMP
    negative regulation of microtubule polymerization or depolymerization GO:0031111 ISS
    negative regulation of microtubule polymerization GO:0031115 IEA
    negative regulation of microtubule polymerization GO:0031115 ISO
    protein-containing complex localization GO:0031503 IDA
    male sex differentiation GO:0046661 IMP
    reproductive structure development GO:0048608 IMP
    chaperone-mediated protein folding GO:0061077 IBA
    chaperone-mediated protein folding GO:0061077 ISO
    chaperone-mediated protein folding GO:0061077 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    microtubule GO:0005874 IEA
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    axonal growth cone GO:0044295 ISO
    axonal growth cone GO:0044295 ISS
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Fkbp4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Fkbp4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Stip1 20867
Co-fractionation Mus musculus
2 ACVR1 90
Affinity Capture-Luminescence Homo sapiens
3 Sugt1 67955
Co-fractionation Mus musculus
4 Hsp90aa1 15519
Two-hybrid Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
6 Ptpn2  
Co-fractionation Mus musculus
7 Ptges3 56351
Co-fractionation Mus musculus
8 SMURF2 64750
Affinity Capture-Luminescence Homo sapiens
9 Fubp3  
Two-hybrid Mus musculus
10 Tfe3  
Affinity Capture-MS Mus musculus
11 Sh3glb2  
Co-fractionation Mus musculus
12 Nr3c1  
Affinity Capture-Western Mus musculus
13 Uba5  
Co-fractionation Mus musculus
14 Twist1  
Proximity Label-MS Mus musculus
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