Gene description for Ptges3
Gene name prostaglandin E synthase 3 (cytosolic)
Gene symbol Ptges3
Other names/aliases 5730442A20Rik
Ptges
Tebp
cPGES
p23
sid3177
Species Mus musculus
 Database cross references - Ptges3
ExoCarta ExoCarta_56351
Vesiclepedia VP_56351
Entrez Gene 56351
UniProt Q9R0Q7  
 Ptges3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ptges3
Molecular Function
    p53 binding GO:0002039 ISO
    telomerase activity GO:0003720 IEA
    telomerase activity GO:0003720 ISO
    enzyme binding GO:0019899 ISO
    prostaglandin-E synthase activity GO:0050220 IBA
    prostaglandin-E synthase activity GO:0050220 ISO
    prostaglandin-E synthase activity GO:0050220 ISS
    unfolded protein binding GO:0051082 ISO
    unfolded protein binding GO:0051082 ISS
    protein-folding chaperone binding GO:0051087 IBA
    Hsp90 protein binding GO:0051879 IBA
    Hsp90 protein binding GO:0051879 IEA
    Hsp90 protein binding GO:0051879 ISO
    DNA polymerase binding GO:0070182 IPI
    DNA polymerase binding GO:0070182 ISO
Biological Process
    prostaglandin biosynthetic process GO:0001516 IBA
    prostaglandin biosynthetic process GO:0001516 IEA
    prostaglandin biosynthetic process GO:0001516 IMP
    prostaglandin biosynthetic process GO:0001516 ISO
    prostaglandin biosynthetic process GO:0001516 ISS
    glycogen biosynthetic process GO:0005978 IMP
    protein folding GO:0006457 IBA
    prostaglandin metabolic process GO:0006693 ISO
    telomere maintenance via telomerase GO:0007004 IBA
    telomere maintenance via telomerase GO:0007004 IDA
    telomere maintenance via telomerase GO:0007004 ISO
    telomere maintenance via telomerase GO:0007004 ISO
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of telomere maintenance via telomerase GO:0032212 IEA
    positive regulation of phosphorylation GO:0042327 ISO
    nuclear receptor-mediated glucocorticoid signaling pathway GO:0042921 IMP
    skin development GO:0043588 IMP
    fibroblast proliferation GO:0048144 IMP
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IEA
    chaperone cofactor-dependent protein refolding GO:0051085 ISO
    chaperone-mediated protein complex assembly GO:0051131 IBA
    chaperone-mediated protein complex assembly GO:0051131 IEA
    chaperone-mediated protein complex assembly GO:0051131 ISO
    neuron apoptotic process GO:0051402 ISO
    lung saccule development GO:0060430 IMP
    telomerase holoenzyme complex assembly GO:1905323 IBA
    telomerase holoenzyme complex assembly GO:1905323 IDA
    telomerase holoenzyme complex assembly GO:1905323 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    telomerase holoenzyme complex GO:0005697 IEA
    telomerase holoenzyme complex GO:0005697 ISO
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 IBA
    actin filament GO:0005884 ISO
    protein-containing complex GO:0032991 ISO
    neuronal cell body GO:0043025 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    protein folding chaperone complex GO:0101031 IEA
    protein folding chaperone complex GO:0101031 ISO
 Experiment description of studies that identified Ptges3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ptges3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rnh1 107702
Co-fractionation Mus musculus
2 Ppp5c  
Co-fractionation Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Gpalpp1  
Co-fractionation Mus musculus
5 GZMB  
Biochemical Activity Homo sapiens
6 Osbp 76303
Co-fractionation Mus musculus
7 Fkbp4 14228
Co-fractionation Mus musculus
8 Eed  
Affinity Capture-MS Mus musculus
9 Dnmt3a  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
10 Pdcl  
Co-fractionation Mus musculus
11 Chaf1a  
Affinity Capture-MS Mus musculus
12 Unk  
Affinity Capture-RNA Mus musculus
13 Aip  
Co-fractionation Mus musculus
14 Ppid 67738
Co-fractionation Mus musculus
15 Casp7  
Biochemical Activity Mus musculus
16 Bax 12028
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
17 Kat2a  
Affinity Capture-Western Mus musculus
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